Phenotypes associated with this allele

• Allele Symbol: Grk2^tm1Mca
• Allele Name: targeted mutation 1, Marc Caron
• Allele ID: MGI:2180127
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Grk2^tm1Mca/Grk2^tm1Mca	involves: 129	MGI:2654809
cn2	Grk2^tm1Gwd/Grk2^tm1Mca Tg(Cd4-cre)1Cwi/0	involves: 129 * C57BL/6 * DBA/2	MGI:5295054

Genotype
MGI:2654809

hm1

• Allelic Composition: Grk2^tm1Mca/Grk2^tm1Mca
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Cardiac abnormalities in Grk2^tm1Mca/Grk2^tm1Mca mouse embryos

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:36567 )

• no homozygotes survive beyond E15.5 as a result of heart failure

embryonic lethality during organogenesis, incomplete penetrance ( J:36567 )

• starting at E9, homozygotes exhibit a progressive decline in embryonic survival up to E15.5

cardiovascular system

abnormal myocardial trabeculae morphology ( J:36567 )

• at E12.5-E15.5, homozygotes display abnormally thinned trabeculae in the ventricles and atria

thin myocardium compact layer ( J:36567 )

• at E12.5-E15.5, homozygotes display an abnormally thin compact myocardial layer

disorganized myocardium ( J:36567 )

• at E12.5-E15.5, homozygotes exhibit absence of organization or differentiation of the ventricular myocardium

thin myocardium ( J:36567 )

heart atrium hypoplasia ( J:36567 )

• at E12.5-E15.5, homozygotes display hypoplasia of the right and left atria

dilated heart right atrium ( J:36567 )

• at E13, homozygotes display a dilated right atrium

abnormal interventricular septum morphology ( J:36567 )

• at E12.5-E15.5, homozygotes display hypoplasia and dysplasia of the interventricular septum, with persistence of an aberrantly wide interventricular foramen

heart hypoplasia ( J:36567 )

• at E12.5-E15.5, homozygotes display hypoplasia and dysplasia of the myocardium

• mutant ventricular and atrial cavities (lumina) appear unusually large as a result of hypoplasia

ventricular hypoplasia ( J:36567 )

• at E12.5-E15.5, homozygotes display hypoplasia of both the right and left ventricles

trabecula carnea hypoplasia ( J:36567 )

• at E12.5-E15.5, homozygotes show disorganized trabeculae in the ventricular wall

thin ventricular wall ( J:36567 )

• in some homozygotes, the nontrabeculated ventricular myocardium is composed of only a single cell layer; in contrast, the endocardial and epicardial layers appear normal

decreased heart left ventricle muscle contractility ( J:36567 )

• at E12.5-E13.5, homozygotes display a >70% decrease in left ventricular ejection fraction, indicating ventricular chamber dysfunction

growth/size/body

decreased embryo size ( J:36567 )

fetal growth retardation ( J:36567 )

• at E15.5, homozygotes display an overall developmental delay of ~24-36 hr relative to wild-type embryos

muscle

abnormal myocardial trabeculae morphology ( J:36567 )

• at E12.5-E15.5, homozygotes display abnormally thinned trabeculae in the ventricles and atria

trabecula carnea hypoplasia ( J:36567 )

• at E12.5-E15.5, homozygotes show disorganized trabeculae in the ventricular wall

thin myocardium compact layer ( J:36567 )

• at E12.5-E15.5, homozygotes display an abnormally thin compact myocardial layer

disorganized myocardium ( J:36567 )

• at E12.5-E15.5, homozygotes exhibit absence of organization or differentiation of the ventricular myocardium

thin myocardium ( J:36567 )

decreased heart left ventricle muscle contractility ( J:36567 )

• at E12.5-E13.5, homozygotes display a >70% decrease in left ventricular ejection fraction, indicating ventricular chamber dysfunction

embryo

decreased embryo size ( J:36567 )

integument

pallor ( J:36567 )

• at E15.5, homozygotes exhibit a normal liver but are generally paler than wild-type or heterozygous embryos, suggesting inadequate vascular irrigation

Genotype
MGI:5295054

cn2

• Allelic Composition: Grk2^tm1Gwd/Grk2^tm1Mca; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: 129 * C57BL/6 * DBA/2

immune system

impaired leukocyte tethering or rolling ( J:176118 )

• lymph node high endothelial venules exhibit a 3- to 4-fold reduction in T cells undergo the rolling-to-sticking transition compared with controls

• T cells exhibit decreased rolling velocity compared with control cells

increased B cell number ( J:176118 )

• in the spleen

decreased T cell number ( J:176118 )

• in the lymph nodes and blood but not spleen

decreased CD4-positive, alpha-beta T cell number ( J:176118 )

• in the blood and lymph nodes

decreased CD8-positive, alpha-beta T cell number ( J:176118 )

• in the blood and lymph nodes

abnormal T cell physiology ( J:176118 )

• T cells exhibit elevated chemotactic response to S1P and decreased response to CCL21 compared with control cells

• T cells exhibit defective entry into the lymph node compared with control cells

• lymph node high endothelial venules exhibit a 3- to 4-fold reduction in T cells undergo the rolling-to-sticking transition compared with controls

• T cells exhibit decreased rolling velocity compared with control cells

hematopoietic system

impaired leukocyte tethering or rolling ( J:176118 )

• lymph node high endothelial venules exhibit a 3- to 4-fold reduction in T cells undergo the rolling-to-sticking transition compared with controls

• T cells exhibit decreased rolling velocity compared with control cells

increased B cell number ( J:176118 )

• in the spleen

decreased T cell number ( J:176118 )

• in the lymph nodes and blood but not spleen

decreased CD4-positive, alpha-beta T cell number ( J:176118 )

• in the blood and lymph nodes

decreased CD8-positive, alpha-beta T cell number ( J:176118 )

• in the blood and lymph nodes

abnormal T cell physiology ( J:176118 )

• T cells exhibit elevated chemotactic response to S1P and decreased response to CCL21 compared with control cells

• T cells exhibit defective entry into the lymph node compared with control cells

• lymph node high endothelial venules exhibit a 3- to 4-fold reduction in T cells undergo the rolling-to-sticking transition compared with controls

• T cells exhibit decreased rolling velocity compared with control cells

cellular

impaired leukocyte tethering or rolling ( J:176118 )

• lymph node high endothelial venules exhibit a 3- to 4-fold reduction in T cells undergo the rolling-to-sticking transition compared with controls

• T cells exhibit decreased rolling velocity compared with control cells