Phenotypes associated with this allele

• Allele Symbol: Adora1^tm1Bbf
• Allele Name: targeted mutation 1, Bertil B Fredholm
• Allele ID: MGI:2180136
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Adora1^tm1Bbf/Adora1^tm1Bbf	B6.129P2-Adora1^tm1Bbf	MGI:5007487
hm2	Adora1^tm1Bbf/Adora1^tm1Bbf	involves: 129P2/OlaHsd * C57BL	MGI:3613555
hm3	Adora1^tm1Bbf/Adora1^tm1Bbf	involves: 129P2/OlaHsd * C57BL/6	MGI:3622796
cn4	Adk^tm2Bois/Adk^tm2Bois Adora1^tm1Bbf/Adora1^tm1Bbf Tg(Nes-cre)1Kln/0	involves: 129P2/OlaHsd * C57BL/6 * SJL	MGI:5816716

Genotype
MGI:5007487

hm1

• Allelic Composition: Adora1^tm1Bbf/Adora1^tm1Bbf
• Genetic Background: B6.129P2-Adora1^tm1Bbf

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

allodynia ( J:149872 )

• in the complete Freund's adjuvant model of chronic inflammatory pain or in the spared nerve injury model of neuropathic pain, mice injected with human ACPP exhibit increased mechanical allodynia compared with wild-type mice

decreased thermal nociceptive threshold ( J:149872 )

• in mice injected with human ACPP

• in mice injected with human ACPP in the complete Freund's adjuvant model of chronic inflammatory pain or in the spared nerve injury model of neuropathic pain

Genotype
MGI:3613555

hm2

• Allelic Composition: Adora1^tm1Bbf/Adora1^tm1Bbf
• Genetic Background: involves: 129P2/OlaHsd * C57BL

homeostasis/metabolism

abnormal body temperature homeostasis ( J:70664 )

• homozygotes exhibit normal body temperature; however, the hypothermia induced by i.p. administration of A₁R agonist N⁶-cyclohexyladenosine (0.1 mg/kg) in wild-type controls (-3.4 +/- 0.16 degrees C) is abolished in mutant mice (-0.08 +/- 0.39 degrees C)

behavior/neurological

increased anxiety-related response ( J:70664 )

• homozygotes display increased anxiety in the dark-light box test, with a significantly reduced number of entries into as well as less total time spent in the lit compartment

• however, visual placing reflex, equilibrium, prehensility or total activity over a 24-h period are normal

hyperalgesia ( J:70664 )

• homozygotes exhibit thermal (but not mechanical) hyperalgesia relative to wild-type or heterozygous mice

decreased thermal nociceptive threshold ( J:70664 )

• homozygotes react faster to thermal pain, as shown by significanlty reduced latencies under basal conditions or after intrathecal administration of adenosine analogue R-PIA in the tail-flick test

• i.p. administration of morphine (5 mg/kg) has no effect on thermal nociception

cardiovascular system

cardiovascular system phenotype ( J:70664 )

N • homozygotes show no significant differences in heart rate or blood pressure relative to wild-type mice

nervous system

abnormal CNS synaptic transmission ( J:70664 )

• homozygotes display complete loss of both adenosine-mediated inhibition and theophylline-mediated augmentation of excitatory glutamatergic neurotransmission

abnormal excitatory postsynaptic currents ( J:70664 )

• electrophysiological recordings from hippocampal slices indicate that homozygotes fail to exhibit adenosine-mediated inhibition of EPSCs

abnormal excitatory postsynaptic potential ( J:70664 )

• electrophysiological recordings from hippocampal slices indicate that homozygotes fail to exhibit adenosine-mediated inhibition of fEPSPs

• in response to hypoxia/anoxia, homozygotes show a significantly delayed and attenuated inhibition of fEPSP responses in hippocampal slices, with no recovery after 60 min of incubation in hypoxic buffer

decreased synaptic depression ( J:70664 )

• homozygotes exhibit a reduced response to hypoxia/anoxia, with mutant hippocampal slices exhibiting a delayed and attenuated decrease in synaptic transmission relative to wild-type slices

respiratory system

abnormal respiratory mechanics ( J:70664 )

• in reponse to hypoxia, brainstem spinal cord preparations from wild-type mice show a decrease in respiratory output within 3 min after the onset of hypoxia, with full recovery of the respiratory rhythm and brainstem neuronal activity upon reintroduction of oxygenated medium; in mutant preparations, the depression in respiratory output is significantly delayed and full recovery is not attained

Genotype
MGI:3622796

hm3

• Allelic Composition: Adora1^tm1Bbf/Adora1^tm1Bbf
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:99612 )

N • response to caffeine induced wakefulness is similar to wild-type mice

Genotype
MGI:5816716

cn4

• Allelic Composition: Adk^tm2Bois/Adk^tm2Bois; Adora1^tm1Bbf/Adora1^tm1Bbf; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * SJL

behavior/neurological

environmentally induced seizures ( J:237189 )

♂ • mice exhibit increased susceptibility to stress-induced (placement in novel environment) seizures

mortality/aging

premature death ( J:237189 )

♂ • increase in mortality

nervous system

environmentally induced seizures ( J:237189 )

♂ • mice exhibit increased susceptibility to stress-induced (placement in novel environment) seizures