Phenotypes associated with this allele

• Allele Symbol: Npas2^tm1Slm
• Allele Name: targeted mutation 1, Steven L McKnight
• Allele ID: MGI:2180392
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Npas2^tm1Slm/Npas2^tm1Slm	B6.129S6-Npas2^tm1Slm	MGI:4362038
hm2	Npas2^tm1Slm/Npas2^tm1Slm	involves: 129S6/SvEvTac	MGI:4822131
hm3	Npas2^tm1Slm/Npas2^tm1Slm	involves: 129S6/SvEvTac * C57BL/6J	MGI:3639439

Genotype
MGI:4362038

hm1

• Allelic Composition: Npas2^tm1Slm/Npas2^tm1Slm
• Genetic Background: B6.129S6-Npas2^tm1Slm

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

decreased heart rate ( J:125921 )

• particularly during the light dark transition

• however, diurnal rhythmicity is maintained

decreased mean systemic arterial blood pressure ( J:125921 )

• particularly during the light dark transition

• diurnal rhythmicity is maintained; however, the acrophase (time of peak) is delayed

hypotension ( J:125921 )

homeostasis/metabolism

decreased circulating adrenaline level ( J:125921 )

• at Zeitgeber time (ZT) 2

decreased circulating noradrenaline level ( J:125921 )

• at ZT2

Genotype
MGI:4822131

hm2

• Allelic Composition: Npas2^tm1Slm/Npas2^tm1Slm
• Genetic Background: involves: 129S6/SvEvTac

cardiovascular system

abnormal blood flow velocity ( J:155089 )

• basaline blood flow is decreased compared to in wild-type mice

homeostasis/metabolism

abnormal circulating protein level ( J:155089 )

• mice exhibit decreased plasminogen activator inhibitor-1 (PAI-1) levels compared with wild-type mice

• at ZT2 and ZT8, mice exhibit decreased total plasma tissue plasminogen activator (tPA) levels compared with wild-type mice

abnormal response to injury ( J:155089 )

• time to thrombotic vascular occlusion (TTVO) subsequent to photochemical injury is faster at zeitgeber time (ZT) 20 than in similarly treated wild-type mice

• TTVO subsequent to photochemical injury is increased at ZT 2, 8, and 14 compared with similarly treated wild-type mice

• however, diurnal variation in TTVO is normal

Genotype
MGI:3639439

hm3

• Allelic Composition: Npas2^tm1Slm/Npas2^tm1Slm
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

behavior/neurological

abnormal learning/memory/conditioning ( J:63035 )

• in an adapted CCF assay, mutants show decreased freezing behavior in an environment where smell and appearance of the cage is novel, but the texture of the cage flooring is identical to that used in training; the cued stimulus used in this 'novel' environment facilitates recall in both mutant and wild-type mice (freezing behavior 45% vs 62% in wild-type)

abnormal contextual conditioning behavior ( J:63035 )

• mutants freeze less often than wild-type in the 24-hour contextual arm of a CCF assay (35% vs 50%), but no differences are seen in the 0.5 hour arm of the assay

abnormal cued conditioning behavior ( J:63035 )

• in the 24-hour cued arm of the CCF assay, mutants exhibit a significant deficit relative to wild-type littermates (40% vs 50%); freezing behavior before the auditory cue is similar between mutant and wild-type

abnormal sleep duration ( J:109589 )

• sleep time is reduced in mutants; initial sustained period of wakefulness in null mice is 7 hours vs 5.9 hours for wild-type

• mice have fewer waking bouts (2.8) than wild-type (3.6) and the average length per waking bout is increased by ~2 hours (237 minutes vs 127 minutes)

• null mice lose ~41 minutes of non REM sleep each day compared to wild-type

abnormal sleep pattern ( J:109589 )

• mice have fewer waking bouts (2.8) than wild-type (3.6) and the average length per waking bout is increased by ~2 hours (237 minutes vs 127 minutes)

• null mice lose ~41 minutes of non REM sleep each day compared to wild-type

• mutant males lag behind female mutants and wild-type mice in recovery of sleep time

• changes in delta frequency range show a 2-fold increase over baseline and was lower in mutants in the lower delta range; ratio of EEG power in fast and slow delta power is generally smaller in mutants and the ratio transiently increased during non REM sleep after prolonged periods of wakefulness more in mutants

abnormal frequency of paradoxical sleep ( J:109589 )

• female mutants exhibit a higher number of transitions from non REM sleep to REM sleep compared to males

• rhythmic EEG activity is altered in mutants

nervous system

abnormal brain wave pattern ( J:109589 )

• during sleep, changes in delta frequency range show a 2-fold increase over baseline and was lower in mutants in the lower delta range; ratio of EEG power in fast and slow delta power is generally smaller in mutants and the ratio transiently increased during non REM sleep after prolonged periods of wakefulness more in mutants