Phenotypes associated with this allele

• Allele Symbol: Cd247^tm1Mal
• Allele Name: targeted mutation 1, Bernard Malissen
• Allele ID: MGI:2180466
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd247^tm1Mal/Cd247^tm1Mal	either: C57BL/6-Cd247^tm1Mal or (involves: BALB/c * C57BL/6)	MGI:4356540
hm2	Cd247^tm1Mal/Cd247^tm1Mal	involves: C57BL/6	MGI:4357682
cx3	Cd247^tm1Mal/Cd247^tm1Mal Lck^tm1Mak/Lck^tm1Mak	involves: 129S2/SvPas * C57BL/6	MGI:4357681

Genotype
MGI:4356540

hm1

• Allelic Composition: Cd247^tm1Mal/Cd247^tm1Mal
• Genetic Background: either: C57BL/6-Cd247^tm1Mal or (involves: BALB/c * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased thymocyte number ( J:15272 )

• thymocyte number is variably decreased by 2- to 30- fold less than controls

• thymocytes express very low levels of TCR

abnormal T cell differentiation ( J:15272 )

• alpha-beta T cell development is partially blocked at the double-negative to the double-positive transition

increased double-negative T cell number ( J:15272 )

• the relative number of double negative thymocytes is increased compared to controls

• a double negative population is found in the lymph nodes

decreased double-positive T cell number ( J:15272 )

• relative and total number of double positive thymocytes is decreased in these mice

decreased CD4-positive, alpha-beta T cell number ( J:15272 )

• CD4 T cell numbers are decreased in the lymph node

decreased CD8-positive, alpha-beta T cell number ( J:15272 )

• CD8 T cell numbers are decreased in the lymph node

decreased single-positive T cell number ( J:15272 )

• single-positive thymocyte numbers are dramatically reduced

abnormal intraepithelial T cell morphology ( J:15272 )

• IEL numbers in the gut are decreased

• these IELs consistently express lower levels of TCR and CD3epsilon

hematopoietic system

decreased thymocyte number ( J:15272 )

• thymocyte number is variably decreased by 2- to 30- fold less than controls

• thymocytes express very low levels of TCR

abnormal T cell differentiation ( J:15272 )

• alpha-beta T cell development is partially blocked at the double-negative to the double-positive transition

increased double-negative T cell number ( J:15272 )

• the relative number of double negative thymocytes is increased compared to controls

• a double negative population is found in the lymph nodes

decreased double-positive T cell number ( J:15272 )

• relative and total number of double positive thymocytes is decreased in these mice

decreased CD4-positive, alpha-beta T cell number ( J:15272 )

• CD4 T cell numbers are decreased in the lymph node

decreased CD8-positive, alpha-beta T cell number ( J:15272 )

• CD8 T cell numbers are decreased in the lymph node

decreased single-positive T cell number ( J:15272 )

• single-positive thymocyte numbers are dramatically reduced

abnormal intraepithelial T cell morphology ( J:15272 )

• IEL numbers in the gut are decreased

• these IELs consistently express lower levels of TCR and CD3epsilon

endocrine/exocrine glands

decreased thymocyte number ( J:15272 )

• thymocyte number is variably decreased by 2- to 30- fold less than controls

• thymocytes express very low levels of TCR

Genotype
MGI:4357682

hm2

• Allelic Composition: Cd247^tm1Mal/Cd247^tm1Mal
• Genetic Background: involves: C57BL/6

immune system

abnormal thymocyte activation ( J:110847 )

• double positive thymocytes do not efficiently enter into the cell cycle during the double positive stage

abnormal T cell morphology ( J:110847 )

• double-positive thymocytes have a slightly higher expression of alpha-beta TCR

abnormal T cell differentiation ( J:112990 )

• fetal thymocyte number fails to increase exponentially in response

• the CD44^-HSA⁺CD25^- DN thymocyte development stage is sometime skipped over

abnormal double-negative T cell morphology ( J:110847 , J:112990 )

• there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes (J:110847)

• the number of CD44^-HSA⁺CD25^- DN thymyoctes is 2% of controls (J:112990)

• less proportion of these cells are actively cycling compared to controls (J:112990)

decreased double-positive T cell number ( J:110847 )

• only 3% of thymocytes are double-positive compared to 97% in controls

hematopoietic system

abnormal thymocyte activation ( J:110847 )

• double positive thymocytes do not efficiently enter into the cell cycle during the double positive stage

abnormal T cell morphology ( J:110847 )

• double-positive thymocytes have a slightly higher expression of alpha-beta TCR

abnormal T cell differentiation ( J:112990 )

• fetal thymocyte number fails to increase exponentially in response

• the CD44^-HSA⁺CD25^- DN thymocyte development stage is sometime skipped over

abnormal double-negative T cell morphology ( J:110847 , J:112990 )

• there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes (J:110847)

• the number of CD44^-HSA⁺CD25^- DN thymyoctes is 2% of controls (J:112990)

• less proportion of these cells are actively cycling compared to controls (J:112990)

decreased double-positive T cell number ( J:110847 )

• only 3% of thymocytes are double-positive compared to 97% in controls

endocrine/exocrine glands

abnormal thymocyte activation ( J:110847 )

• double positive thymocytes do not efficiently enter into the cell cycle during the double positive stage

Genotype
MGI:4357681

cx3

• Allelic Composition: Cd247^tm1Mal/Cd247^tm1Mal; Lck^tm1Mak/Lck^tm1Mak
• Genetic Background: involves: 129S2/SvPas * C57BL/6

immune system

abnormal thymocyte activation ( J:110847 )

• minimal numbers of double positive thymocytes enter into the cell cycle during the double positive stage

abnormal T cell morphology ( J:110847 )

• thymocytes fail to express an alphabeta TCR

abnormal T cell differentiation ( J:110847 )

• there is a partial block in development in the DN3 stage that is more severe in the double homozygote null mice than in the singly- homozygote null mice

abnormal double-negative T cell morphology ( J:110847 )

• there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes

decreased double-positive T cell number ( J:110847 )

• double-positive thymocytes are almost absent from the thymus in 6 week old mice

• the double-positive thymocyte population is slightly larger by nine weeks of age

hematopoietic system

abnormal thymocyte activation ( J:110847 )

• minimal numbers of double positive thymocytes enter into the cell cycle during the double positive stage

abnormal T cell morphology ( J:110847 )

• thymocytes fail to express an alphabeta TCR

abnormal T cell differentiation ( J:110847 )

• there is a partial block in development in the DN3 stage that is more severe in the double homozygote null mice than in the singly- homozygote null mice

abnormal double-negative T cell morphology ( J:110847 )

• there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes

decreased double-positive T cell number ( J:110847 )

• double-positive thymocytes are almost absent from the thymus in 6 week old mice

• the double-positive thymocyte population is slightly larger by nine weeks of age

endocrine/exocrine glands

abnormal thymocyte activation ( J:110847 )

• minimal numbers of double positive thymocytes enter into the cell cycle during the double positive stage