Phenotypes associated with this allele

• Allele Symbol: Cd247^tm1Tks
• Allele Name: targeted mutation 1, Takashi Saito
• Allele ID: MGI:2180484
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd247^tm1Tks/Cd247^tm1Tks	B6.129P2-Cd247^tm1Tks	MGI:4356493
hm2	Cd247^tm1Tks/Cd247^tm1Tks	involves: 129P2/OlaHsd * C57BL/6	MGI:4356474

Genotype
MGI:4356493

hm1

• Allelic Composition: Cd247^tm1Tks/Cd247^tm1Tks
• Genetic Background: B6.129P2-Cd247^tm1Tks

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased NK T cell number ( J:28282 )

• NK T cells numbers are decreased in the thymus

• alpha-beta NK T cells are virtually absent in the thymus while there is a six-fold increase in gamma-delta NK T cells

• these gamma-delta NK T cells have similar NK markers as their alpha-beta counterparts except most are CD4^-

abnormal NK cell morphology ( J:66403 )

• the expression of FcgRIII is increased on NK cells

abnormal NK cell physiology ( J:66403 )

• NK cells secrete increased amounts of IFN-gamma relative to controls in response to immobilized IgG

increased natural killer cell mediated cytotoxicity ( J:66403 )

• Ab-dependent cell-mediated cytotoxicity by NK cells is enhanced in these mice

• cytolytic activity against NK target cells is normal

increased interferon-gamma secretion ( J:66403 )

• NK cells secrete increased amounts of IFN-gamma relative to controls in response to immobilized IgG

hematopoietic system

decreased NK T cell number ( J:28282 )

• NK T cells numbers are decreased in the thymus

• alpha-beta NK T cells are virtually absent in the thymus while there is a six-fold increase in gamma-delta NK T cells

• these gamma-delta NK T cells have similar NK markers as their alpha-beta counterparts except most are CD4^-

abnormal NK cell morphology ( J:66403 )

• the expression of FcgRIII is increased on NK cells

abnormal NK cell physiology ( J:66403 )

• NK cells secrete increased amounts of IFN-gamma relative to controls in response to immobilized IgG

increased natural killer cell mediated cytotoxicity ( J:66403 )

• Ab-dependent cell-mediated cytotoxicity by NK cells is enhanced in these mice

• cytolytic activity against NK target cells is normal

Genotype
MGI:4356474

hm2

• Allelic Composition: Cd247^tm1Tks/Cd247^tm1Tks
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal T cell proliferation ( J:15271 )

• T cells do not proliferate to the same degree as controls when stimulated with ConA or anti-CD3e

• proliferative rates to PMA + Ionomycin are normal

small thymus medulla ( J:15271 )

• the thymus medullary is severely reduced in these mice

decreased thymocyte number ( J:15271 )

• thymocyte number is reduced about 6-fold

decreased double-positive T cell number ( J:15271 )

• double positive thymocyte number is decreased about 7-fold

decreased CD4-positive, alpha-beta T cell number ( J:15271 )

• peripheral CD4 T cell numbers are reduced about 10-fold at weaning though numbers approach 1/3rd of normal by 14 weeks of age

decreased CD8-positive, alpha-beta T cell number ( J:15271 )

• CD8 T cell numbers are less than controls at weaning but approach normal levels by 14 weeks of age

decreased single-positive T cell number ( J:15271 )

• the CD4 single-positive thymocyte population is 1/16th that found in wild-type

• the CD8 single positive thymocyte population is 1/6th that found in wild-type

abnormal alpha-beta intraepithelial T cell morphology ( J:15271 )

• alpha-beta IEL numbers are decreased in the gut

hematopoietic system

abnormal T cell proliferation ( J:15271 )

• T cells do not proliferate to the same degree as controls when stimulated with ConA or anti-CD3e

• proliferative rates to PMA + Ionomycin are normal

small thymus medulla ( J:15271 )

• the thymus medullary is severely reduced in these mice

decreased thymocyte number ( J:15271 )

• thymocyte number is reduced about 6-fold

decreased double-positive T cell number ( J:15271 )

• double positive thymocyte number is decreased about 7-fold

decreased CD4-positive, alpha-beta T cell number ( J:15271 )

• peripheral CD4 T cell numbers are reduced about 10-fold at weaning though numbers approach 1/3rd of normal by 14 weeks of age

decreased CD8-positive, alpha-beta T cell number ( J:15271 )

• CD8 T cell numbers are less than controls at weaning but approach normal levels by 14 weeks of age

decreased single-positive T cell number ( J:15271 )

• the CD4 single-positive thymocyte population is 1/16th that found in wild-type

• the CD8 single positive thymocyte population is 1/6th that found in wild-type

abnormal alpha-beta intraepithelial T cell morphology ( J:15271 )

• alpha-beta IEL numbers are decreased in the gut

endocrine/exocrine glands

small thymus medulla ( J:15271 )

• the thymus medullary is severely reduced in these mice

decreased thymocyte number ( J:15271 )

• thymocyte number is reduced about 6-fold

cellular

abnormal T cell proliferation ( J:15271 )

• T cells do not proliferate to the same degree as controls when stimulated with ConA or anti-CD3e

• proliferative rates to PMA + Ionomycin are normal