hematopoietic system
N |
• homozygotes displayed normal bleeding times
• at 8-12 weeks of age, homozygotes had normal platelet numbers in peripheral blood
• early events of platelet activation (e.g. shape change and granule release) and the initial rate of platelet aggregation appeared normal
• no differences were detected in the cleavage pattern and kinetics of calpain substrates
|
• platelets showed impaired tyrosine phosphorylation of several proteins upon activation with either calcium ionophore or thrombin, including the beta3 subunit of alphaIIb-beta3 integrin (~70% reduction)
|
• platelet aggregation was decreased by 50-60% in response to thrombin (10 nM), ADP (20 uM), collagen (20 ug/ml), and calcium ionophore A23187 (1.0 uM)
|
• subtle differences were detected in the ATP secretion from dense granules upon thrombin and collagen activation; no differences were detected in the ADP- and calcium ionophore A23187-treated platelets
|
homeostasis/metabolism
• platelets were defective in retracting clots, especially when clot formation was induced with 1.0 nM thrombin
|
• platelets showed impaired tyrosine phosphorylation of several proteins upon activation with either calcium ionophore or thrombin, including the beta3 subunit of alphaIIb-beta3 integrin (~70% reduction)
|
• platelet aggregation was decreased by 50-60% in response to thrombin (10 nM), ADP (20 uM), collagen (20 ug/ml), and calcium ionophore A23187 (1.0 uM)
|
• subtle differences were detected in the ATP secretion from dense granules upon thrombin and collagen activation; no differences were detected in the ADP- and calcium ionophore A23187-treated platelets
|