Phenotypes associated with this allele

• Allele Symbol: Ccnd2^tm1Wbg
• Allele Name: targeted mutation 1, Robert A Weinberg
• Allele ID: MGI:2180642
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ccnd2^tm1Wbg/Ccnd2^tm1Wbg	involves: 129S2/SvPas	MGI:2657220
hm2	Ccnd2^tm1Wbg/Ccnd2^tm1Wbg	involves: 129S2/SvPas * C57BL/6J	MGI:2657239
cx3	Ccnd1^tm1Dsn/Ccnd1^tm1Dsn Ccnd2^tm1Wbg/Ccnd2^tm1Wbg Ccnd3^tm1Pisc/Ccnd3^tm1Pisc	involves: 129P2/OlaHsd * 129S2/SvPas	MGI:3514155
cx4	Ccnd1^tm1Phin/Ccnd1^tm1Phin Ccnd2^tm1Wbg/Ccnd2^tm1Wbg	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6	MGI:3614894
cx5	Ccnd2^tm1Wbg/Ccnd2^tm1Wbg Ccnd3^tm1Pisc/Ccnd3^tm1Pisc	Not Specified	MGI:3036215
cx6	Ccnd1^tm1Wbg/Ccnd1^tm1Wbg Ccnd2^tm1Wbg/Ccnd2^tm1Wbg	Not Specified	MGI:3036177

Genotype
MGI:2657220

hm1

• Allelic Composition: Ccnd2^tm1Wbg/Ccnd2^tm1Wbg
• Genetic Background: involves: 129S2/SvPas

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal ovarian folliculogenesis ( J:37092 )

♀ • hypoplastic follicles with fewer granulosa cells

♀ • inability of granulosa cells to proliferate in response to FSH

decreased testis weight ( J:37092 )

♂ • weighed 2.5 times less than those of wild-type mice

reproductive system

oligozoospermia ( J:37092 )

♂ • 2.0-2.9 fold decrease

abnormal ovarian folliculogenesis ( J:37092 )

♀ • hypoplastic follicles with fewer granulosa cells

♀ • inability of granulosa cells to proliferate in response to FSH

decreased testis weight ( J:37092 )

♂ • weighed 2.5 times less than those of wild-type mice

abnormal ovulation ( J:37092 )

♀ • normal oocytes that could be fertilized in vitro were trapped in corpora lutea

female infertility ( J:37092 )

♀ • males were fertile

cellular

oligozoospermia ( J:37092 )

♂ • 2.0-2.9 fold decrease

Genotype
MGI:2657239

hm2

• Allelic Composition: Ccnd2^tm1Wbg/Ccnd2^tm1Wbg
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

nervous system

abnormal cerebellum morphology ( J:52358 )

abnormal cerebellar foliation ( J:52358 )

• folia are foreshortened or stunted

abnormal cerebellum external granule cell layer morphology ( J:52358 )

• reduction in the proportion of EGL cells in S-phase; proliferative index within the postnatal EGL is decreased by 36% and 41% at P3 and P14, respectively

• apoptosis is increased in the EGL

thin external granule cell layer ( J:52358 )

• the external granular layer (EGL) begins to appear thin at the tips of forming folia at P3, lacks the well-defined, palisading appearance of the wild-type, and is markedly thinner at both the folial tips and clefts by P14

abnormal Purkinje cell morphology ( J:52358 )

• Purkinje cells labeled with calbindin antibody are more closely packed than in wild-type

abnormal Purkinje cell dendrite morphology ( J:52358 )

• primary dendritic branches of Purkinje cells appear thicker and secondary arborization in the outer molecular layer is simplified compared to wild-type

abnormal cerebellar granule layer morphology ( J:52358 )

• 50.8% decrease in the area of the internal granular layer (IGL) relative to wild-type controls

• reduction in granule cell number due to both decreased neurogenesis and increased apoptosis

• granule cells are loosely packed in the IGL compared to controls

abnormal cerebellar molecular layer ( J:52358 )

• near absence of stellate interneurons, however the overall thickness of the layer is comparable to that of wild-type

small cerebellum ( J:52358 )

Genotype
MGI:3514155

cx3

• Allelic Composition: Ccnd1^tm1Dsn/Ccnd1^tm1Dsn; Ccnd2^tm1Wbg/Ccnd2^tm1Wbg; Ccnd3^tm1Pisc/Ccnd3^tm1Pisc
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:92563 )

• by E15.5 embryos appear pale

• by E16.5 no viable embryos are observed

cardiovascular system

abnormal interventricular septum morphology ( J:92563 )

• embryonic

thin ventricular wall ( J:92563 )

• thinned walls of embryonic heart ventricles, mainly in compact zone

hematopoietic system

abnormal definitive hematopoiesis ( J:92563 )

• total number of fetal liver cells reduced at E14.5

abnormal common myeloid progenitor cell morphology ( J:92563 )

• common myeloid progenitors cells substantially reduced

abnormal lymphopoiesis ( J:92563 )

• common lymphoid progenitors cells reduced by 46 fold

anemia ( J:92563 )

decreased erythrocyte cell number ( J:92563 )

• E14.5 embryos exhibit 8-fold decrease in peripheral blood erythrocytes

abnormal hematopoietic stem cell morphology ( J:92563 )

• impaired ability of hematopoietic stem cells to proliferate

decreased hematopoietic stem cell number ( J:92563 )

• hematopoietic stem cells reduced 5.7 fold in E14.5 liver

homeostasis/metabolism

hydrops fetalis ( J:92563 )

• embryonic

immune system

abnormal lymphopoiesis ( J:92563 )

• common lymphoid progenitors cells reduced by 46 fold

Genotype
MGI:3614894

cx4

• Allelic Composition: Ccnd1^tm1Phin/Ccnd1^tm1Phin; Ccnd2^tm1Wbg/Ccnd2^tm1Wbg
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae * C57BL/6

nervous system

abnormal cerebellum development ( J:105045 )

• display severely retarded cerebellar development

Genotype
MGI:3036215

cx5

• Allelic Composition: Ccnd2^tm1Wbg/Ccnd2^tm1Wbg; Ccnd3^tm1Pisc/Ccnd3^tm1Pisc
• Genetic Background: Not Specified

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:80994 )

• fetuses died during late gestation with none surviving past E18.5

hematopoietic system

abnormal erythropoiesis ( J:80994 )

macrocytic anemia ( J:80994 )

low mean erythrocyte cell number ( J:80994 )

• ~4 fold decrease in the number of peripheral red blood cells relative to wild-type

macrocytosis ( J:80994 )

liver/biliary system

abnormal liver development ( J:80994 )

• abnormal fetal liver morphogenesis

Genotype
MGI:3036177

cx6

• Allelic Composition: Ccnd1^tm1Wbg/Ccnd1^tm1Wbg; Ccnd2^tm1Wbg/Ccnd2^tm1Wbg
• Genetic Background: Not Specified

mortality/aging

postnatal lethality ( J:80994 )

behavior/neurological

impaired coordination ( J:80994 )

vision/eye

retina hypoplasia ( J:80994 )

nervous system

reduced cerebellar foliation ( J:80994 )

• decreased number of folia

• existing folia were stunted with interfolial clefts

ectopic Purkinje cell ( J:80994 )

• aberrantly located in the molecular and internal granule layers

absent Purkinje cell layer ( J:80994 )

• absence of a proper layer

abnormal cerebellar granule layer morphology ( J:80994 )

• decreased thickness

small cerebellum ( J:80994 )

• impaired development resulting in rudimentary formation