Phenotypes associated with this allele

• Allele Symbol: Tcra^tm1Mjo
• Allele Name: targeted mutation 1, Michael J Owen
• Allele ID: MGI:2180883
• Summary: 13 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tcra^tm1Mjo/Tcra^tm1Mjo	involves: 129P2/OlaHsd	MGI:4944216
hm2	Tcra^tm1Mjo/Tcra^tm1Mjo	involves: 129P2/OlaHsd * BALB/c	MGI:3618333
hm3	Tcra^tm1Mjo/Tcra^tm1Mjo	involves: 129P2/OlaHsd * MRL	MGI:4947950
hm4	Tcra^tm1Mjo/Tcra^tm1Mjo	NOD.129P2-Tcra^tm1Mjo	MGI:3623759
ht5	Tcra^tm1Mjo/Tcra^+	NOD.129P2-Tcra^tm1Mjo	MGI:4944217
ht6	Tcra^tm1Mjo/Tcra^+	SJL.129P2-Tcra^tm1Mjo	MGI:4944219
cx7	Tcra^tm1Mjo/Tcra^tm1Mjo X/Yaa	BXSB.129P2(Cg)-Tcra^tm1Mjo/Theo	MGI:6154363
cx8	Fas^lpr/Fas^lpr Tcra^tm1Mjo/Tcra^tm1Mjo	involves: 129P2/OlaHsd * 129S2/SvPas * MRL	MGI:4947940
cx9	Cd40lg^tm1Flv/Y Fas^lpr/Fas^lpr Tcra^tm1Mjo/Tcra^tm1Mjo	involves: 129P2/OlaHsd * 129S2/SvPas * MRL	MGI:4947943
cx10	Cd40lg^tm1Flv/Cd40lg^tm1Flv Fas^lpr/Fas^lpr Tcra^tm1Mjo/Tcra^tm1Mjo	involves: 129P2/OlaHsd * 129S2/SvPas * MRL	MGI:4947944
cx11	Mpz^tm1Msch/Mpz^+ Tcra^tm1Mjo/Tcra^tm1Mjo	involves: 129P2/OlaHsd * 129S7/SvEvBrd * BALB/c * C57BL/6	MGI:4947953
cx12	Fas^lpr/Fas^lpr Tcra^tm1Mjo/Tcra^tm1Mjo	involves: 129P2/OlaHsd * MRL	MGI:4944220
cx13	Fas^lpr/Fas^lpr Tcra^tm1Mjo/Tcra^+	MRL.Cg-Tcra^tm1Mjo Fas^lpr	MGI:4944218

Genotype
MGI:4944216

hm1

• Allelic Composition: Tcra^tm1Mjo/Tcra^tm1Mjo
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell proliferation ( J:110898 )

• proliferation in response to stimulation with the mitogen Con A is reduced compared to heterozygous controls

colitis ( J:37699 )

• in specific pathogen free mice areas of the colonic mucosa with architectural disturbances also show marked neutrophilic infiltration

• inflammation is not detected in germ free or gnotobiotic mice

enlarged spleen ( J:110898 )

• in mice removed from specific pathogen free conditions and exposed to environmental antigens compared to heterozygous controls

spleen hyperplasia ( J:110898 )

• increase mean cell yield in mice removed from specific pathogen free conditions and exposed to environmental antigens compared to heterozygous controls

increased T cell number ( J:110898 )

• increase in the abundance of TCRB+ TCRA- cells in mice exposed to environmental antigens

increased gamma-delta T cell number ( J:110898 )

• increase in gamma-delta T cells in the spleen, mesenteric lymph nodes, Peyer's patch lymph nodes and peripheral lymph nodes in mice exposed to environmental antigens compared to heterozygous controls

• a substantial proportion of the gamma-delta T cells are activated

increased activated T cell number ( J:110898 )

• a substantial proportion of the gamma-delta T cells in lymph organs of mice exposed to environmental antigens are activated

enlarged mesenteric lymph nodes ( J:110898 )

• in mice removed from specific pathogen free conditions and exposed to environmental antigens compared to heterozygous controls

digestive/alimentary system

diarrhea ( J:37699 )

• occasionally seen in specific pathogen free mice

abnormal intestinal mucosa morphology ( J:37699 )

• in specific pathogen free mice areas of the colonic mucosa show hyperplasia, an increase in crypt fusion and crowding of the glands

• however, colon morphology is similar to wild-type controls in germ free or gnotobiotic mice

abnormal colon morphology ( J:37699 )

• in specific pathogen free mice areas of the colonic mucosa show hyperplasia, an increase in crypt fusion and crowding of the glands

rectal prolapse ( J:37699 )

• occasionally seen in specific pathogen free mice

colitis ( J:37699 )

• in specific pathogen free mice areas of the colonic mucosa with architectural disturbances also show marked neutrophilic infiltration

• inflammation is not detected in germ free or gnotobiotic mice

nervous system

abnormal neuron proliferation ( J:170591 )

• decrease in hippocampal neurogenesis

hematopoietic system

decreased T cell proliferation ( J:110898 )

• proliferation in response to stimulation with the mitogen Con A is reduced compared to heterozygous controls

enlarged spleen ( J:110898 )

• in mice removed from specific pathogen free conditions and exposed to environmental antigens compared to heterozygous controls

spleen hyperplasia ( J:110898 )

• increase mean cell yield in mice removed from specific pathogen free conditions and exposed to environmental antigens compared to heterozygous controls

increased T cell number ( J:110898 )

• increase in the abundance of TCRB+ TCRA- cells in mice exposed to environmental antigens

increased gamma-delta T cell number ( J:110898 )

• increase in gamma-delta T cells in the spleen, mesenteric lymph nodes, Peyer's patch lymph nodes and peripheral lymph nodes in mice exposed to environmental antigens compared to heterozygous controls

• a substantial proportion of the gamma-delta T cells are activated

increased activated T cell number ( J:110898 )

• a substantial proportion of the gamma-delta T cells in lymph organs of mice exposed to environmental antigens are activated

cellular

decreased T cell proliferation ( J:110898 )

• proliferation in response to stimulation with the mitogen Con A is reduced compared to heterozygous controls

abnormal neuron proliferation ( J:170591 )

• decrease in hippocampal neurogenesis

growth/size/body

enlarged spleen ( J:110898 )

• in mice removed from specific pathogen free conditions and exposed to environmental antigens compared to heterozygous controls

spleen hyperplasia ( J:110898 )

• increase mean cell yield in mice removed from specific pathogen free conditions and exposed to environmental antigens compared to heterozygous controls

Genotype
MGI:3618333

hm2

• Allelic Composition: Tcra^tm1Mjo/Tcra^tm1Mjo
• Genetic Background: involves: 129P2/OlaHsd * BALB/c

immune system

increased B cell number ( J:111129 )

• in spleens compared to heterozygous controls

abnormal T cell morphology ( J:1292 )

• alphabeta+ T cells are completely absent

increased activated T cell number ( J:111129 )

• on average 27% of the gamma-delta T cells are activated

increased immunoglobulin level ( J:111129 )

increased IgE level ( J:111129 )

• in some mice compared to heterozygous controls

increased IgG1 level ( J:111129 )

• in some mice compared to heterozygous controls

abnormal immune system organ morphology ( J:1292 )

abnormal thymus cortex morphology ( J:1292 )

• the cortices of thymi in nulls are greatly expanded and tightly packed with CD4+CD8+ cells

enlarged thymus cortex ( J:1292 )

absent thymus medulla ( J:1292 )

• no medullae are discernible in mutant thymi

abnormal splenic cell ratio ( J:1292 )

• elimination of alphabeta+ T cells is compensated for by an increase in splenic immunoglobulin positive cells (B cells) positive for IgM and IgD

small Peyer's patches ( J:1292 )

• in mutants, these are smaller and shriveled in appearance compared to wild-type, being approximately half the size of controls

increased autoantibody level ( J:111129 )

• about 50% of sera are reactive for mammalian cell proteins compared to only about 11% of sera from heterozygous controls

• reactivity in some mice is primarily against small nuclear ribonucleoproteins

• by 5 weeks of age about 50% of mice show IgG autoantibodies in more than one assay (anti-dsDNA, anti-nuclear antigens, and immunoblotting for antibodies to mammalian cell proteins)

increased anti-nuclear antigen antibody level ( J:111129 )

• more than 80% of sera are positive for anti-nuclear antigens although the degree of reactivity varies

increased anti-double stranded DNA antibody level ( J:111129 )

hematopoietic system

abnormal thymus cortex morphology ( J:1292 )

• the cortices of thymi in nulls are greatly expanded and tightly packed with CD4+CD8+ cells

enlarged thymus cortex ( J:1292 )

absent thymus medulla ( J:1292 )

• no medullae are discernible in mutant thymi

increased B cell number ( J:111129 )

• in spleens compared to heterozygous controls

abnormal T cell morphology ( J:1292 )

• alphabeta+ T cells are completely absent

increased activated T cell number ( J:111129 )

• on average 27% of the gamma-delta T cells are activated

abnormal splenic cell ratio ( J:1292 )

• elimination of alphabeta+ T cells is compensated for by an increase in splenic immunoglobulin positive cells (B cells) positive for IgM and IgD

increased immunoglobulin level ( J:111129 )

increased IgE level ( J:111129 )

• in some mice compared to heterozygous controls

increased IgG1 level ( J:111129 )

• in some mice compared to heterozygous controls

endocrine/exocrine glands

abnormal thymus cortex morphology ( J:1292 )

• the cortices of thymi in nulls are greatly expanded and tightly packed with CD4+CD8+ cells

enlarged thymus cortex ( J:1292 )

absent thymus medulla ( J:1292 )

• no medullae are discernible in mutant thymi

Genotype
MGI:4947950

hm3

• Allelic Composition: Tcra^tm1Mjo/Tcra^tm1Mjo
• Genetic Background: involves: 129P2/OlaHsd * MRL

renal/urinary system

increased urine protein level ( J:45448 )

• compared to age-matched B10.A mice

abnormal kidney morphology ( J:45448 )

• develop glomerular, interstitial and sometimes perivascular lesions

homeostasis/metabolism

increased urine protein level ( J:45448 )

• compared to age-matched B10.A mice

Genotype
MGI:3623759

hm4

• Allelic Composition: Tcra^tm1Mjo/Tcra^tm1Mjo
• Genetic Background: NOD.129P2-Tcra^tm1Mjo

immune system

increased activated T cell number ( J:52940 )

• when splenic T cells from BDC2.5 transgenic mice are transferred to Tcra deficient mice, significant proliferation of transferred splenocytes is detected in the pancreatic lymph nodes but not in the spleen or other lymph nodes 3 days after transfer

• when T cells from adult BDC2.5 transgenics are transferred to 10 day old or adult Tcra-deficient NOD mice, proliferation of T cells is detected after 3 days only in adult hosts in pancreatic lymph nodes

hematopoietic system

increased activated T cell number ( J:52940 )

• when splenic T cells from BDC2.5 transgenic mice are transferred to Tcra deficient mice, significant proliferation of transferred splenocytes is detected in the pancreatic lymph nodes but not in the spleen or other lymph nodes 3 days after transfer

• when T cells from adult BDC2.5 transgenics are transferred to 10 day old or adult Tcra-deficient NOD mice, proliferation of T cells is detected after 3 days only in adult hosts in pancreatic lymph nodes

Genotype
MGI:4944217

ht5

• Allelic Composition: Tcra^tm1Mjo/Tcra⁺
• Genetic Background: NOD.129P2-Tcra^tm1Mjo

immune system

abnormal T cell physiology ( J:108590 )

• unlike in wild-type controls no dual TCRA expressing cells are present

decreased susceptibility to autoimmune diabetes ( J:108590 )

• males and females are significantly protected from cyclophosphamide induced diabetes compared to wild-type controls

• marker analysis confirms that multiple susceptibility loci are entirely NOD derived

hematopoietic system

abnormal T cell physiology ( J:108590 )

• unlike in wild-type controls no dual TCRA expressing cells are present

Genotype
MGI:4944219

ht6

• Allelic Composition: Tcra^tm1Mjo/Tcra⁺
• Genetic Background: SJL.129P2-Tcra^tm1Mjo

immune system

immune system phenotype ( J:108590 )

N • no difference in the susceptibility to experimental autoimmune encephalomyelitis is detected compared to wild-type controls

Genotype
MGI:6154363

cx7

• Allelic Composition: Tcra^tm1Mjo/Tcra^tm1Mjo; X/Yaa
• Genetic Background: BXSB.129P2(Cg)-Tcra^tm1Mjo/Theo

immune system

immune system phenotype ( J:120153 )

♂N • due to the absense of alpha/beta T cells these Yaa-bearing males do not develop hypergammaglobulinemia, anti-chromatin auto-antibodies, enlarged lymph nodes or spleen, hemolytic anemia, immune complex glomerulonephritis, monocytosis, or the lupus-like autoimmune disease that normally causes premature death in Yaa-bearing males on the BXSB background, but instead these males are reported to live beyond 300 days

Genotype
MGI:4947940

cx8

• Allelic Composition: Fas^lpr/Fas^lpr; Tcra^tm1Mjo/Tcra^tm1Mjo
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * MRL

mortality/aging

extended life span ( J:38492 )

• relative to mutant mice wild-type for Tcra

immune system

decreased immunoglobulin level ( J:38492 )

• lower titers of immunoglobulins, other than IgE, compared to mutant mice wild-type for Tcra

decreased autoantibody level ( J:38492 )

• lower titers autoantibodies compared to mutant mice wild-type for Tcra

integument

skin lesions ( J:38492 )

• development of skin lesions is delayed (develop by 7 to 8 months of age) compared to mutant mice wild-type by Tcra

renal/urinary system

abnormal kidney morphology ( J:38492 )

• substantial reduction in renal disease compared to mutant mice wild-type for Tcra

hematopoietic system

decreased immunoglobulin level ( J:38492 )

• lower titers of immunoglobulins, other than IgE, compared to mutant mice wild-type for Tcra

Genotype
MGI:4947943

cx9

• Allelic Composition: Cd40lg^tm1Flv/Y; Fas^lpr/Fas^lpr; Tcra^tm1Mjo/Tcra^tm1Mjo
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * MRL

immune system

decreased autoantibody level ( J:38492 )

♂ • lower titers autoantibodies compared to mutant mice wild-type for Cd40lg

♂ • decrease in the anti-snRNP and anti-Ig titers relative to mutant mice compared to mutant mice wild-type for Cd40lg

increased anti-double stranded DNA antibody level ( J:38492 )

♂ • relative to mutant mice wild-type for Tcra

integument

integument phenotype ( J:38492 )

♂N • do not develop the skin lesions seen in mutant mice wild-type for Tcra and/or Cd40lg

renal/urinary system

abnormal kidney morphology ( J:38492 )

♂ • renal disease is decreased compared to mutant mice wild-type Cd40lg but more diseased than mutant mice wild-type for Tcra

Genotype
MGI:4947944

cx10

• Allelic Composition: Cd40lg^tm1Flv/Cd40lg^tm1Flv; Fas^lpr/Fas^lpr; Tcra^tm1Mjo/Tcra^tm1Mjo
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * MRL

immune system

decreased autoantibody level ( J:38492 )

♀ • lower titers autoantibodies compared to mutant mice wild-type for Cd40lg

♀ • decrease in the anti-snRNP and anti-Ig titers relative to mutant mice compared to mutant mice wild-type for Cd40lg

increased anti-double stranded DNA antibody level ( J:38492 )

♀ • relative to mutant mice wild-type for Tcra

integument

integument phenotype ( J:38492 )

♀N • do not develop the skin lesions seen in mutant mice wild-type for Tcra and/or Cd40lg

renal/urinary system

abnormal kidney morphology ( J:38492 )

♀ • renal disease is decreased compared to mutant mice wild-type Cd40lg but more diseased than mutant mice wild-type for Tcra

Genotype
MGI:4947953

cx11

• Allelic Composition: Mpz^tm1Msch/Mpz⁺; Tcra^tm1Mjo/Tcra^tm1Mjo
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd * BALB/c * C57BL/6

nervous system

increased Schwann cell number ( J:59319 )

• increase in the number of Schwann cells around the quadriceps femoral nerves at 13 months of age is less than that in age matched mutant mice heterozygous for the Tcra null allele

abnormal neuron morphology ( J:59319 )

• pathological lesions indicative of compromised myelin maintenance in the quadriceps femoral nerves at 13 months of age are less severe than those in age matched mutant mice heterozygous for the Tcra null allele

abnormal myelin sheath morphology ( J:59319 )

• myelin sheaths of quadriceps femoral nerves at 13 months of age are thicker than those in age matched mutant mice heterozygous for the Tcra null allele

abnormal nervous system physiology ( J:59319 )

• fewer macrophages are found in quadriceps nerves compared to mutant mice heterozygous for the Tcra null allele

abnormal nerve conduction ( J:59319 )

• impaired conduction with prolonged latencies of M-responses and of F waves after distal or proximal stimulations

• impairment tends to be less severe than in mutant mice heterozygous for the Tcra null allele

decreased nerve conduction velocity ( J:59319 )

• reduction is less severe than in mutant mice heterozygous for the Tcra null allele

immune system

decreased T cell number ( J:59319 )

• absence of mature alpha beta lymphocytes

hematopoietic system

decreased T cell number ( J:59319 )

• absence of mature alpha beta lymphocytes

Genotype
MGI:4944220

cx12

• Allelic Composition: Fas^lpr/Fas^lpr; Tcra^tm1Mjo/Tcra^tm1Mjo
• Genetic Background: involves: 129P2/OlaHsd * MRL

immune system

immune system phenotype ( J:26250 )

N • unlike in mutant mice wild-type for Tcra, massive lymphadenopathy is not seen at 16 weeks of age

increased T cell number ( J:26250 )

• 6 fold increase in TCRB+ cells compared to mutant mice wild-type for Fas

• within the TCRB+ cell population the proportion of DN B220+ cells is increased compared mutant mice wild-type for Fas

increased gamma-delta T cell number ( J:26250 )

• 15 fold expansion at 16 weeks of age compared to mutant mice wild-type for Fas

• the majority of these cells express B220 and DN as a result of preferential expansion (300 fold) of this population of cells

increased gamma-delta intraepithelial T cell number ( J:26250 )

• increase in the proportion of T cells in the peripheral lymph nodes expressing the gamma delta variable regions typical of intestinal intraepithelial T cell compared to mutant mice wild-type for Fas

kidney inflammation ( J:45448 )

• periglomerular infiltration and perivasculitis

renal/urinary system

increased urine protein level ( J:45448 )

• compared to age-matched B10.A mice

kidney inflammation ( J:45448 )

• periglomerular infiltration and perivasculitis

abnormal kidney morphology ( J:45448 )

• develop glomerular, interstitial and sometimes perivascular lesions

• lesion development is reduced compared mutant mice wild-type for Tcra

abnormal renal glomerulus morphology ( J:45448 )

• focal glomerular hypercellularity

homeostasis/metabolism

increased blood urea nitrogen level ( J:45448 )

• significantly elevated

increased urine protein level ( J:45448 )

• compared to age-matched B10.A mice

hematopoietic system

increased T cell number ( J:26250 )

• 6 fold increase in TCRB+ cells compared to mutant mice wild-type for Fas

• within the TCRB+ cell population the proportion of DN B220+ cells is increased compared mutant mice wild-type for Fas

increased gamma-delta T cell number ( J:26250 )

• 15 fold expansion at 16 weeks of age compared to mutant mice wild-type for Fas

• the majority of these cells express B220 and DN as a result of preferential expansion (300 fold) of this population of cells

increased gamma-delta intraepithelial T cell number ( J:26250 )

• increase in the proportion of T cells in the peripheral lymph nodes expressing the gamma delta variable regions typical of intestinal intraepithelial T cell compared to mutant mice wild-type for Fas

Genotype
MGI:4944218

cx13

• Allelic Composition: Fas^lpr/Fas^lpr; Tcra^tm1Mjo/Tcra⁺
• Genetic Background: MRL.Cg-Tcra^tm1Mjo Fas^lpr

immune system

immune system phenotype ( J:108590 )

N • no difference in the susceptibility to systemic lupus erythematosus is detected compared to wild-type controls