Analysis Tools
mortality/aging
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• homozygotes display postimplantation embryonic lethality between days 3.5 and 8.5 pc
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cellular
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• at days 5.5 and 6.5, Giemsa-stained, degenerating cultured mutant embryos show severe chromosomal missegregation
• at day 5.5, cultured embryos exhibit a substantial number of micronuclei, formed from lagging chromosomes; macronuclei are also formed
• at day 6.5, most cells appear macronucleated, suggesting impaired chromosome segregation and cytokinesis
• at day 6.5, mitotic defects include nuclear bridging and blebbing of the nuclear membrane; chromatin fragmentation and hypercondensation are also apparent
• chromosomes of mutant embryos appear more condensed and scattered than those of wild-type embryos
• at day 6.5, no mitotic chromosomes are identifiable, suggesting cessation of mitosis at this stage
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embryo
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• at 5.5 days pc, a number of cultured mutant embryos show rapid deterioration of the inner cell mass
• by 6.5 days, these mutant embryos lack an identifiable inner cell mass
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• at 6.5 days pc, a number of cultured mutant embryos show a significant reduction in trophectoderm cell number
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