Phenotypes associated with this allele

• Allele Symbol: Cry2^tm1Asn
• Allele Name: targeted mutation 1, Aziz Sancar
• Allele ID: MGI:2181212
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cry2^tm1Asn/Cry2^tm1Asn	involves: 129P2/OlaHsd * C57BL/6	MGI:2450986
cx2	Cry1^tm1Asn/Cry1^tm1Asn Cry2^tm1Asn/Cry2^tm1Asn	involves: 129P2/OlaHsd	MGI:3789955
cx3	Cry2^tm1Asn/Cry2^tm1Asn Pde6b^rd1/Pde6b^rd1	involves: 129P2/OlaHsd	MGI:3789958
cx4	Cry2^tm1Asn/Cry2^tm1Asn Fbxl3^tm1.1Ljp/Fbxl3^tm1.1Ljp	involves: 129P2/OlaHsd	MGI:5494571
cx5	Cry1^tm1Asn/Cry1^tm1Asn Cry2^tm1Asn/Cry2^tm1Asn Pde6b^rd1/Pde6b^rd1	involves: 129P2/OlaHsd * C3H/HeJ * C57BL/6	MGI:2451102
cx6	Cry1^tm1Asn/Cry1^tm1Asn Cry2^tm1Asn/Cry2^tm1Asn	involves: 129P2/OlaHsd * C57BL/6	MGI:2450985

Genotype
MGI:2450986

hm1

• Allelic Composition: Cry2^tm1Asn/Cry2^tm1Asn
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

prolonged circadian behavior period ( J:51110 , J:58114 )

• circadian period 1 hour longer for motor activity than wild-type in constant darkness (J:51110)

• capable of entrainment to light, but with more variance than wild-type controls (J:51110)

abnormal circadian behavior persistence ( J:58114 )

• significant reduction in the relative power of the circadian range is seen when placed in constant darkness

Genotype
MGI:3789955

cx2

• Allelic Composition: Cry1^tm1Asn/Cry1^tm1Asn; Cry2^tm1Asn/Cry2^tm1Asn
• Genetic Background: involves: 129P2/OlaHsd

skeleton

increased osteoblast cell number ( J:115188 )

increased bone mass ( J:115188 )

• in the long bones and vertebrae

vision/eye

vision/eye phenotype ( J:81500 )

N • pupilary reflex is the same as in wild-type controls

Genotype
MGI:3789958

cx3

• Allelic Composition: Cry2^tm1Asn/Cry2^tm1Asn; Pde6b^rd1/Pde6b^rd1
• Genetic Background: involves: 129P2/OlaHsd

vision/eye

impaired pupillary reflex ( J:81500 )

• substantial loss of constriction in response to light

• response is similar to that in mice homozygous for Pde6b^rd1 alone

behavior/neurological

impaired pupillary reflex ( J:81500 )

• substantial loss of constriction in response to light

• response is similar to that in mice homozygous for Pde6b^rd1 alone

Genotype
MGI:5494571

cx4

• Allelic Composition: Cry2^tm1Asn/Cry2^tm1Asn; Fbxl3^tm1.1Ljp/Fbxl3^tm1.1Ljp
• Genetic Background: involves: 129P2/OlaHsd

behavior/neurological

prolonged circadian behavior period ( J:194250 )

• activity period of 28.2 hours suggesting a lack of interaction between these two genes

Genotype
MGI:2451102

cx5

• Allelic Composition: Cry1^tm1Asn/Cry1^tm1Asn; Cry2^tm1Asn/Cry2^tm1Asn; Pde6b^rd1/Pde6b^rd1
• Genetic Background: involves: 129P2/OlaHsd * C3H/HeJ * C57BL/6

Retinal degeneration in Pde6b^rd1/Pde6b^rd1, and Pde6b^rd1/Pde6b^rd1 Cry1^tm1Asn/Cry1^tm1Asn Cry2^tm1Asn/Cry2^tm1Asn mouse retinas

behavior/neurological

impaired pupillary reflex ( J:81500 )

• almost no pupillary reflex in blue light (470-nm)

• some pupillary movement in very bright light, but sluggish

• sensitivity to blue light is about 5% that of mice homozygous for Pde6b^rd1 only

abnormal locomotor circadian rhythm ( J:66580 )

• arrhythmic circadian rhythms for locomotor activity in constant darkness

decreased locomotor activity ( J:66580 )

abnormal circadian behavior phase ( J:66580 )

• arrhythmic circadian rhythms for locomotor activity in light/dark cycles

• mutants accumulate nearly 40% of their total daily activity during the first 10 hours of the light portion of light-dark 12:12 compared with only 10% of activity during this same period seen with controls, indicating a residual photoresponsiveness in mutants

vision/eye

impaired pupillary reflex ( J:81500 )

• almost no pupillary reflex in blue light (470-nm)

• some pupillary movement in very bright light, but sluggish

• sensitivity to blue light is about 5% that of mice homozygous for Pde6b^rd1 only

abnormal retina morphology ( J:66580 )

decreased retina cone cell number ( J:66580 )

• most cones are absent

absent retina rod cells ( J:66580 )

absent photoreceptor outer segment ( J:66580 )

abnormal retina outer nuclear layer morphology ( J:66580 )

• nearly complete absence of the outer nuclear layer

retina degeneration ( J:66580 )

• the entire outer retina is destroyed, however the inner retina remains intact

nervous system

decreased retina cone cell number ( J:66580 )

• most cones are absent

absent retina rod cells ( J:66580 )

absent photoreceptor outer segment ( J:66580 )

Genotype
MGI:2450985

cx6

• Allelic Composition: Cry1^tm1Asn/Cry1^tm1Asn; Cry2^tm1Asn/Cry2^tm1Asn
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

behavior/neurological

arrhythmic circadian behavior persistence ( J:58114 )

• immediate loss of rhythmicity when switched to constant darkness

adipose tissue

decreased subcutaneous adipose tissue amount ( J:128437 )

• dramatic decrease in subcutaneous fat mass

decreased abdominal adipose tissue amount ( J:128437 )

• dramatic decrease in visceral fat mass

decreased total body fat amount ( J:128437 )

• total body electrical conductivity is significantly decreased indicating a decrease in total body fat mass

abnormal brown adipose tissue morphology ( J:128437 )

• lipid droplet size is decreased

growth/size/body

decreased body weight ( J:128437 )

• seen from 5 weeks of age on

homeostasis/metabolism

abnormal circadian temperature homeostasis ( J:128437 )

• body temperature remains elevated during the light phase of the day-night cycle

• absence of diurnal rhythms in body temperature under 12 h dark light conditions

increased oxygen consumption ( J:128437 )

• increased oxygen consumption during the light phase

• however, no significant difference is seen in respiratory quotient or carbohydrate consumption compared to wild-type controls

impaired glucose tolerance ( J:128437 )

• the peak level of glucose is delayed, maximal blood glucose level is increased, and plasma insulin levels at 15 and 30 min. after glucose challenge are decreased

increased insulin sensitivity ( J:128437 )

• plasma glucose levels are significantly lower during an insulin tolerance test

• however, pancreatic insulin secretory response to glucose are similar to wild-type controls

cardiovascular system

abnormal circadian regulation of heart rate ( J:128437 )

• heart rate remains elevated during the light phase of the day-night cycle

• absence of diurnal rhythms in heart rate under 12 h dark light conditions

integument

decreased subcutaneous adipose tissue amount ( J:128437 )

• dramatic decrease in subcutaneous fat mass