Phenotypes associated with this allele

• Allele Symbol: Tg(Camk2a-cre)1Gsc
• Allele Name: transgene insertion 1, Gunther Schutz
• Allele ID: MGI:2181422
• Summary: 13 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gna11^tm1Soff/Gna11^tm1Soff Gnaq^tm2Soff/Gnaq^tm2Soff Tg(Camk2a-cre)1Gsc/0	involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 * FVB/N	MGI:3796547
cn2	Adcyap1r1^tm1.2Gsc/Adcyap1r1^tm1.2Gsc Tg(Camk2a-cre)1Gsc/0	involves: 129P2/OlaHsd * FVB/N	MGI:2447757
cn3	Creb1^tm3Gsc/Creb1^tm3Gsc Crem^tm1Gsc/Crem^tm1Gsc Tg(Camk2a-cre)1Gsc/0	involves: 129P2/OlaHsd * FVB/N	MGI:4843915
cn4	Gria2^tm3Rsp/Gria2^tm3Rsp Tg(Camk2a-cre)1Gsc/0	involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * FVB/N	MGI:3701766
cn5	Ncam1^tm1Msch/Ncam1^tm1Msch Tg(Camk2a-cre)1Gsc/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3575508
cn6	Gria2^tm2Rsp/Gria2^+ Tg(Camk2a-cre)1Gsc/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3686594
cn7	Gria2^tm2Rsp/Gria2^+ Tg(Camk2a-cre)1Gsc/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N * NMRI	MGI:3612397
cn8	Grin2b^tm1Mony/Grin2b^tm1Mony Tg(Camk2a-cre)1Gsc/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:5432104
cn9	L1cam^tm1Fmo/Y Tg(Camk2a-cre)1Gsc/0	involves: 129/Sv * C57BL/6	MGI:2682910
cn10	Rhot1^tm1c(EUCOMM)Wtsi/Rhot1^tm1c(EUCOMM)Wtsi Tg(Camk2a-cre)1Gsc/0	involves: C57BL/6Dnk * C57BL/6N * FVB/N	MGI:5906217
cn11	Gria2^tm3Rsp/Gria2^tm3Rsp Tg(Camk2a-cre)1Gsc/0	involves: C57BL/6 * FVB/N	MGI:3612398
cn12	Afdn^tm2Ytk/Afdn^tm2Ytk Tg(Camk2a-cre)1Gsc/0	involves: FVB/N	MGI:4354299
cn13	Chl1^tm1Msch/Chl1^tm1Msch Tg(Camk2a-cre)1Gsc/0	involves: FVB/N	MGI:3848446

Genotype
MGI:3796547

cn1

• Allelic Composition: Gna11^tm1Soff/Gna11^tm1Soff; Gnaq^tm2Soff/Gnaq^tm2Soff; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:111559 )

• age-dependent reduction in survival due to tonic-clonic seizures

nervous system

seizures ( J:111559 )

• around 3 months of age, mice show spontaneous epileptic seizures; animals affected and seizure number per animal increase with age

• mutants show reduced latency to seizure onset and tendency to have stronger seizures when treated with kainic acid or PTZ than wild-type; seizure-induced lethality is greatly increased

• blockade of cannabinoid receptors does not result in aggravation of kainic acid-induced seizures in mutants but does in wild-type controls

• treatment of mutants with endocannabinoid reuptake inhibitor causes a significant amelioration of kainic acid induced seizures

clonic seizures ( J:111559 )

• most seizures are myoclonic

tonic-clonic seizures ( J:111559 )

• some animals exhibit tonic-clonic seizures with age

hippocampal neuron degeneration ( J:111559 )

• older animals particularly those with symptomatic epilepsy show neuronal degeneration in CA1 region

gliosis ( J:111559 )

• older mice display reactive gliosis in CA1 region of hippocampus

abnormal nervous system electrophysiology ( J:123222 )

• no CCK4-induced (cholecystokinin receptor₂ agonist) current is detected in amygdala slices while inward current response in wild-type is unaffected

behavior/neurological

seizures ( J:111559 )

• around 3 months of age, mice show spontaneous epileptic seizures; animals affected and seizure number per animal increase with age

• mutants show reduced latency to seizure onset and tendency to have stronger seizures when treated with kainic acid or PTZ than wild-type; seizure-induced lethality is greatly increased

• blockade of cannabinoid receptors does not result in aggravation of kainic acid-induced seizures in mutants but does in wild-type controls

• treatment of mutants with endocannabinoid reuptake inhibitor causes a significant amelioration of kainic acid induced seizures

clonic seizures ( J:111559 )

• most seizures are myoclonic

tonic-clonic seizures ( J:111559 )

• some animals exhibit tonic-clonic seizures with age

Genotype
MGI:2447757

cn2

• Allelic Composition: Adcyap1r1^tm1.2Gsc/Adcyap1r1^tm1.2Gsc; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129P2/OlaHsd * FVB/N

behavior/neurological

abnormal contextual conditioning behavior ( J:76715 )

• decreased freezing response in long-term test of contextual fear conditioning

Genotype
MGI:4843915

cn3

• Allelic Composition: Creb1^tm3Gsc/Creb1^tm3Gsc; Crem^tm1Gsc/Crem^tm1Gsc; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129P2/OlaHsd * FVB/N

behavior/neurological

behavior/neurological phenotype ( J:142518 )

N • mutants exhibit a normal response to cocaine, even though cocaine-induced transcription is blunted

Genotype
MGI:3701766

cn4

• Allelic Composition: Gria2^tm3Rsp/Gria2^tm3Rsp; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * FVB/N

behavior/neurological

behavior/neurological phenotype ( J:111689 )

N

abnormal spatial reference memory ( J:111689 )

• reference memory is impaired; mice take longer to show improvement in performance in Y-maze test and additional training does not improve success rate compared to controls

abnormal spatial working memory ( J:111689 )

• working memory is impaired; T-maze performance is impaired compared to controls

nervous system

abnormal neuron differentiation ( J:111689 )

• almost no mitotic cells and few postmitotic cells are detected in subgranular zone (SGZ)

abnormal brain interneuron morphology ( J:111689 )

• a significant reduction in parvalbumin-positive interneurons in the dentate gyrus is observed in mutants

loss of hippocampal neurons ( J:111689 )

• loss of pyramidal hippocampal neurons in CA3 is detected with no loss apparent in Gria2^tm2Rsp/+ transgenic mice

abnormal excitatory postsynaptic currents ( J:111689 )

• EPSCs are significantly smaller in mutants than in controls

abnormal excitatory postsynaptic potential ( J:111689 )

• evoked field EPSPs are severely reduced, reaching only ~37% of control values

• stimulation strength needed to elicit presynaptic fiber volleys of given amplitudes are significantly increased vs controls

• synaptic excitability in increased compared to controls; threshold for generating population spike is reduced by 71%

• threshold for generating population spikes are reached in almost all experiments in controls while threshold is reached less than half of experiments for mutants

abnormal AMPA-mediated synaptic currents ( J:111689 )

• in conditional mice, quantification of inward rectifying currents in CA1 neurons at P42 shows a 20-fold increase vs control neurons

abnormal paired-pulse facilitation ( J:111689 )

• once a population spike is elicited in the first response, appearance of multiple spikes is always seen in second response

cellular

abnormal neuron differentiation ( J:111689 )

• almost no mitotic cells and few postmitotic cells are detected in subgranular zone (SGZ)

Genotype
MGI:3575508

cn5

• Allelic Composition: Ncam1^tm1Msch/Ncam1^tm1Msch; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

behavior/neurological

behavior/neurological phenotype ( J:96990 )

N • no overt behavioral abnormalities

N • no deficits in short- and long-term memory in global landmark navigation in the water maze test

nervous system

reduced long-term potentiation ( J:96990 )

• in the CA1 region of the hippocampus

• the deficit in LTP could be rescued by elevation of extracellular Ca<2+> concentrations

• LTP in the CA3 region was normal

absent long-term depression ( J:96990 )

• in the CA1 region of the hippocampus

Genotype
MGI:3686594

cn6

• Allelic Composition: Gria2^tm2Rsp/Gria2⁺; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

behavior/neurological

seizures ( J:111689 )

• seizure episodes are observed, while none are seen in forebrain-specific knockouts

nervous system

seizures ( J:111689 )

• seizure episodes are observed, while none are seen in forebrain-specific knockouts

abnormal neuron differentiation ( J:111689 )

• 2-fold increase in mitotic and postmitotic neurogenic cells is observed in subgranular zone (SGZ)

abnormal brain interneuron morphology ( J:111689 )

• there is loss of interneurons in hippocampus

loss of hippocampal neurons ( J:111689 )

• loss of somatostatin-positive interneurons in hilus and stratum oriens of the CA1 is observed

abnormal CNS glial cell morphology ( J:111689 )

• there are an increased number of glia seen in the amydala, motor cortex and somatosensory cortex

cellular

abnormal neuron differentiation ( J:111689 )

• 2-fold increase in mitotic and postmitotic neurogenic cells is observed in subgranular zone (SGZ)

Genotype
MGI:3612397

cn7

• Allelic Composition: Gria2^tm2Rsp/Gria2⁺; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N * NMRI

behavior/neurological

abnormal olfactory discrimination memory ( J:103689 )

• exhibit more rapid odor learning and enhanced olfactory discrimination in a go/no-go operant conditioning task compared to controls

Genotype
MGI:5432104

cn8

• Allelic Composition: Grin2b^tm1Mony/Grin2b^tm1Mony; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

behavior/neurological

abnormal learning/memory/conditioning ( J:186311 )

• mice exhibit impaired egocentric spatial memory acquisition compared with control mice

abnormal discrimination learning ( J:186311 )

• impaired visual discrimination learning

abnormal object recognition memory ( J:186311 )

• impaired

abnormal spatial learning ( J:186311 )

• impaired in a Morris water maze

abnormal spatial reference memory ( J:186311 )

• impaired in a Morris water maze and appetitive, elevated Y maze

abnormal spatial working memory ( J:186311 )

• impaired in a discrete trial, spontaneous alternation paradigm in an enclosed T maze or a reward alternation on an elevated T maze

decreased anxiety-related response ( J:186311 )

• more so than in Grin2b^tm1Mony/Grin2b^tm1Mony Tg(Camk2a-Grin2c/itTA)12Rps Tg(tetO-cre)LC1Bjd mice

abnormal motor coordination/balance ( J:186311 )

• improved performance on a rotarod

hyperactivity ( J:186311 )

• more so than in Grin2b^tm1Mony/Grin2b^tm1Mony Tg(Camk2a-Grin2c/itTA)12Rps Tg(tetO-cre)LC1Bjd mice

nervous system

nervous system phenotype ( J:186311 )

N • mice exhibit normal cortical layer 4 whisker barrel structures and paired-pilse facilitation

abnormal excitatory postsynaptic currents ( J:186311 )

• ifenprodil-treated mice fail to exhibit an acceleration of NMDAR-mediated excitatory postsynaptic currents compared with wild-type mice

abnormal excitatory postsynaptic potential ( J:186311 )

• at high stimulation frequency, mice exhibit smaller maximal depolarization of the excitatory postsynaptic potentiation (EPSP) envelop and EPSP integral compared with control mice

• however, mice exhibit normal input resistance, action potential threshold, firing pattern and maximal firing frequency

abnormal NMDA-mediated synaptic currents ( J:186311 )

• mice fail to exhibit a reduction in NMDA currents in response to the Grin2b antagonist ifenprodil unlike control mice

• mice exhibit a faster deactivation kinetics (weighted tau) compared with control mice

• ifenprodil-treated mice fail to exhibit an acceleration of NMDAR-mediated excitatory postsynaptic currents compared with wild-type mice

reduced long-term potentiation ( J:186311 )

• low-frequency synaptic stimulation long term potentiation is reduced compared to in control mice

• during long term potentiation induction, charge transfer is reduced compared to in control mice

abnormal miniature excitatory postsynaptic currents ( J:186311 )

• miniature excitatory postsynaptic current amplitudes 50 ms are attenuated after onset unlike in control mice

growth/size/body

decreased body weight ( J:186311 )

• slightly

Genotype
MGI:2682910

cn9

• Allelic Composition: L1cam^tm1Fmo/Y; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129/Sv * C57BL/6

behavior/neurological

increased exploration in new environment ( J:86626 )

♂

abnormal spatial learning ( J:86626 )

♂ • place learning in a water maze was altered compared to control animals

decreased anxiety-related response ( J:86626 )

♂ • reduced thigmotaxis

hyperactivity ( J:86626 )

♂ • increased movement in a new environment

♂ • enter the open arms of an elevated maze more readily

increased vertical activity ( J:86626 )

♂ • increased rearing in a new environment

nervous system

abnormal CNS synaptic transmission ( J:86626 )

♂ • increased basal synaptic activity in the CA1 region

♂ • larger evoked potentials

Genotype
MGI:5906217

cn10

• Allelic Composition: Rhot1^{tm1c(EUCOMM)Wtsi}/Rhot1^{tm1c(EUCOMM)Wtsi}; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: C57BL/6Dnk * C57BL/6N * FVB/N
• Cell Lines: EPD0066_2_F01

nervous system

decreased brain size ( J:240500 )

• at 8 months

enlarged brain ventricles ( J:240500 )

small hippocampus ( J:240500 )

• at 8 months

thin cerebral cortex ( J:240500 )

• at 8 months

astrocytosis ( J:240500 )

• at 8 months

abnormal dendrite morphology ( J:240500 )

• decreased length at 8 months

• increased branchpoints in the proximal region of apical dendrites but decreased branchpoints in distal regions at 8 months

• however, dendrite morphology is normal at 4 months

decreased neuron number ( J:240500 )

• at 8 months

neurodegeneration ( J:240500 )

• at 8 months, mice exhibit enhanced loss of dendritic tree in CA1 pyramidal neurons and decreased numbers of neurons compared with control mice

abnormal axonal transport ( J:240500 )

• reduced mitochondria in the distal dendrites of CA1 neurons at 4 months

Genotype
MGI:3612398

cn11

• Allelic Composition: Gria2^tm3Rsp/Gria2^tm3Rsp; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: C57BL/6 * FVB/N

behavior/neurological

abnormal learning/memory/conditioning ( J:103689 )

• exhibit decreased olfactory memory

abnormal olfactory discrimination memory ( J:103689 )

• exhibit more rapid odor learning and enhanced olfactory discrimination in a go/no-go operant conditioning task, and decreased olfactory memory

increased exploration in new environment ( J:103689 )

• exploratory behavior in an open field task is slightly increased

abnormal spatial learning ( J:103689 )

• acquisition of a nonolfactory hippocampus-dependent spatial learning task (elevated Y-maze) is slightly impaired

increased anxiety-related response ( J:103689 )

• dark/light box tests reveal increased anxiety

impaired coordination ( J:103689 )

• motor coordination in an accelerating rotarod is somewhat impaired

Genotype
MGI:4354299

cn12

• Allelic Composition: Afdn^tm2Ytk/Afdn^tm2Ytk; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: FVB/N

nervous system

abnormal synapse morphology ( J:151436 )

• the number of puncta adherentia junctions per bouton is decreased compared to in wild-type mice

• the number of perforated synapses is increased compared to in wild-type mice

• however, the number of postsynaptic densities per bouton is normal

Genotype
MGI:3848446

cn13

• Allelic Composition: Chl1^tm1Msch/Chl1^tm1Msch; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: FVB/N

behavior/neurological

behavior/neurological phenotype ( J:143051 )

N • mice exhibit normal learning performance in Lashley Maze, passive avoidance, odor discrimination, spatial water maze, and fear conditioning

N • mice exhibit normal sensory and motor ability

abnormal learning/memory/conditioning ( J:143051 )

• mice exhibit an impairment in working memory duration compared with wild-type mice

• however, selective attention is normal

abnormal spatial working memory ( J:143051 )

• performance in a radial arm maze is worse than for wild-type mice

abnormal response to novel object ( J:143051 )

• mice do not spend increased time exploring a novel object unlike wild-type mice