Phenotypes associated with this allele

• Allele Symbol: Stat5a^tm1Jni
• Allele Name: targeted mutation 1, James N Ihle
• Allele ID: MGI:2181607
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Stat5a^tm1Jni/Stat5a^tm1Jni	either: (involves: 129P2/OlaHsd) or (involves: 129X1/SvJ)	MGI:3578098
cx2	Stat5a^tm1Jni/Stat5a^tm1Jni Stat5b^tm2Jni/Stat5b^tm2Jni	either: (involves: 129P2/OlaHsd) or (involves: 129X1/SvJ)	MGI:3578104
cx3	Stat5a^tm1Jni/Stat5a^tm1Jni Stat5b^tm2Jni/Stat5b^tm2Jni	involves: 129 * C57BL/6	MGI:3833588

Genotype
MGI:3578098

hm1

• Allelic Composition: Stat5a^tm1Jni/Stat5a^tm1Jni
• Genetic Background: either: (involves: 129P2/OlaHsd) or (involves: 129X1/SvJ)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

abnormal fat pad morphology ( J:77412 )

• reduced in size

endocrine/exocrine glands

abnormal branching of the mammary ductal tree ( J:77412 )

♀ • reduced alveolar development in response to pregnancy

abnormal mammary gland physiology ( J:77412 )

♀ • reduced levels of alpha-lactalbumin and whey acidic protein

integument

abnormal branching of the mammary ductal tree ( J:77412 )

♀ • reduced alveolar development in response to pregnancy

abnormal mammary gland physiology ( J:77412 )

♀ • reduced levels of alpha-lactalbumin and whey acidic protein

Genotype
MGI:3578104

cx2

• Allelic Composition: Stat5a^tm1Jni/Stat5a^tm1Jni; Stat5b^tm2Jni/Stat5b^tm2Jni
• Genetic Background: either: (involves: 129P2/OlaHsd) or (involves: 129X1/SvJ)

immune system

abnormal myelopoiesis ( J:77412 )

• induction of colony forming cells by Il3 reduced to 57% of controls

• induction of colony forming cells by Il5 reduced to 25% of controls

• induction of colony forming cells by GM-CSF reduced to 47% of controls

increased neutrophil cell number ( J:77412 )

• increased percentage of neutrophiles

decreased leukocyte cell number ( J:77412 )

• due to a decreased percentage of lymphocytes

decreased T cell number ( J:77412 )

• peripheral T cell deficiency

endocrine/exocrine glands

decreased corpora lutea number ( J:77412 )

♀ • few or no large corpora lutea form after ovulation

reproductive system

decreased corpora lutea number ( J:77412 )

♀ • few or no large corpora lutea form after ovulation

female infertility ( J:77412 )

♀

adipose tissue

abnormal fat pad morphology ( J:77412 )

• reduced in size

liver/biliary system

abnormal liver physiology ( J:77412 )

• Major Urinary Protein undetectable in females and dramatically reduced in males

growth/size/body

decreased body weight ( J:77412 )

• weight reduced 20-30% relative to controls in females

• weight reduced 30-40% relative to controls in males

hematopoietic system

abnormal myelopoiesis ( J:77412 )

• induction of colony forming cells by Il3 reduced to 57% of controls

• induction of colony forming cells by Il5 reduced to 25% of controls

• induction of colony forming cells by GM-CSF reduced to 47% of controls

increased neutrophil cell number ( J:77412 )

• increased percentage of neutrophiles

decreased leukocyte cell number ( J:77412 )

• due to a decreased percentage of lymphocytes

decreased T cell number ( J:77412 )

• peripheral T cell deficiency

Genotype
MGI:3833588

cx3

• Allelic Composition: Stat5a^tm1Jni/Stat5a^tm1Jni; Stat5b^tm2Jni/Stat5b^tm2Jni
• Genetic Background: involves: 129 * C57BL/6

mortality/aging

premature death ( J:122463 )

• 40% of the mice die within the first few months

immune system

abnormal mast cell differentiation ( J:84889 )

• bone marrow cells die of apoptosis when cultured in WEHI-3 cell-conditioned media that normally supports mast cell differentiation

• mutant bone marrow cells fail to produce mast cells as wild-type bone marrow does when transferred into mast cell deficient kit^w-v mice

• however, mast cells can be derived from bone marrow cells when cultured with IL-3 and SCF

absent mast cells ( J:84889 )

• mast cells are absent in these mice as determined by histology and flow cytometry

decreased CD4-positive, CD25-positive, alpha-beta regulatory T cell number ( J:122463 )

• reduction of CD4+CD25+ suppressor T cells

abnormal mast cell physiology ( J:84889 )

• bone marrow derived mast cells die of apoptosis when culture is supplemented with only low levels of IL-3 or SCF

• 80-fold more IL-3 or 50-fold more SCF is needed than controls to prevent apoptosis in cultured mast cells

• mast cells delay entering into S-phase when cultured with SCF or IL-3 taking almost twice as long as to reach half-maximal entry into S phase

• these mast cells survive and proliferate when cultured with IL-3 and SCF

hematopoietic system

abnormal mast cell differentiation ( J:84889 )

• bone marrow cells die of apoptosis when cultured in WEHI-3 cell-conditioned media that normally supports mast cell differentiation

• mutant bone marrow cells fail to produce mast cells as wild-type bone marrow does when transferred into mast cell deficient kit^w-v mice

• however, mast cells can be derived from bone marrow cells when cultured with IL-3 and SCF

absent mast cells ( J:84889 )

• mast cells are absent in these mice as determined by histology and flow cytometry

decreased CD4-positive, CD25-positive, alpha-beta regulatory T cell number ( J:122463 )

• reduction of CD4+CD25+ suppressor T cells

abnormal mast cell physiology ( J:84889 )

• bone marrow derived mast cells die of apoptosis when culture is supplemented with only low levels of IL-3 or SCF

• 80-fold more IL-3 or 50-fold more SCF is needed than controls to prevent apoptosis in cultured mast cells

• mast cells delay entering into S-phase when cultured with SCF or IL-3 taking almost twice as long as to reach half-maximal entry into S phase

• these mast cells survive and proliferate when cultured with IL-3 and SCF

cellular

abnormal mast cell differentiation ( J:84889 )

• bone marrow cells die of apoptosis when cultured in WEHI-3 cell-conditioned media that normally supports mast cell differentiation

• mutant bone marrow cells fail to produce mast cells as wild-type bone marrow does when transferred into mast cell deficient kit^w-v mice

• however, mast cells can be derived from bone marrow cells when cultured with IL-3 and SCF