Phenotypes associated with this allele

• Allele Symbol: Ptger2^tm2Bhk
• Allele Name: targeted mutation 2, Beverly H Koller
• Allele ID: MGI:2181614
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptger2^tm2Bhk/Ptger2^tm2Bhk	involves: 129P2/OlaHsd	MGI:5439487
hm2	Ptger2^tm2Bhk/Ptger2^tm2Bhk	involves: 129P2/OlaHsd * 129S/SvEv	MGI:3589827

Genotype
MGI:5439487

hm1

• Allelic Composition: Ptger2^tm2Bhk/Ptger2^tm2Bhk
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

cardiovascular system phenotype ( J:77409 )

♂N • at 5 months of age, male homozygotes show no significant differences mean arterial blood pressure relative to wild-type controls

renal/urinary system

renal/urinary system phenotype ( J:77409 )

♂N • at 5 months of age, male homozygotes show no significant differences in kidney weight or baseline renal hemodynamics, as shown by normal resting renal blood flow (RBF) and renal vascular resistance (RVR) relative to wild-type controls

♂N • after local PGE2 (500 ng) injection into the renal artery, male homozygotes display a similar degree of renal vasodilation as wild-type controls, with a comparable maximum increase in RBF (60-80 s)

Genotype
MGI:3589827

hm2

• Allelic Composition: Ptger2^tm2Bhk/Ptger2^tm2Bhk
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv

reproductive system

reduced female fertility ( J:55713 )

♀ • after 12 weeks of mating, only 18% of mutant females achieve pregnancy and deliver litters, compared with 93% of wild-type females

♀ • in contrast, mutant males remain fully fertile

decreased litter size ( J:55713 )

• when mutant females do become pregnant, they deliver smaller litters than wild-type mice

impaired fertilization ( J:55713 )

• mutant females exhibit significantly reduced fertilization rates in the absence of an intrinsic ovum defect, as in vitro fertilization rates of mutant and wild-type ova are comparable

• impaired fertilization is not associated with hemodynamic changes (i.e. reduced blood pressure)

cardiovascular system

hypotension ( J:55713 )

• on a normal diet (0.4% NaCl), conscious 3-4-mo-old females exhibit a 13 mmHg reduction in resting systolic blood pressure relative to wild-type mice

• hypotension can be partially overcome by increased dietary salt (6% NaCl)

• notably, plasma sodium levels and urinary PGE2 excretion remain unaffected

homeostasis/metabolism

abnormal renin activity ( J:55713 )

• homozygotes fail to exhibit increased plasma renin activity or renin mRNA levels as a compensatory response to reduced blood pressure

immune system

immune system phenotype ( J:78964 )

N • at day 10, homozygotes show no significant differences in the course or severity of collagen antibody-induced rheumatoid arthritis relative to their respective genetic wild-type controls, based on paw swelling/redness and joint ankylosis