Phenotypes associated with this allele

• Allele Symbol: Fos^tm1Wag
• Allele Name: targeted mutation 1, Erwin F Wagner
• Allele ID: MGI:2181817
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fos^tm1Wag/Fos^tm1Wag	involves: 129S2/SvPas	MGI:3700959
hm2	Fos^tm1Wag/Fos^tm1Wag	involves: 129S2/SvPas * C57BL/6	MGI:3700975
ht3	Fos^tm1Wag/Fos^tm2(Fosl1)Wag	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6	MGI:3850715
cx4	Fos^tm1Wag/Fos^tm1Wag Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129S2/SvPas * C57BL/6	MGI:3700989
cx5	Fos^tm1Wag/Fos^tm1Wag Trp53^tm1Tyj/Trp53^+	involves: 129S2/SvPas * C57BL/6	MGI:3700990

Genotype
MGI:3700959

hm1

• Allelic Composition: Fos^tm1Wag/Fos^tm1Wag
• Genetic Background: involves: 129S2/SvPas

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

perinatal lethality, incomplete penetrance ( J:65766 )

• mice exhibit reduced viability at birth

skeleton

skeleton phenotype ( J:118753 )

N • when mice are transplanted with Nfatc1^tm1Mak, osteopetrosis is ameliorated and bone marrow cavities are observed

failure of tooth eruption ( J:65766 )

decreased osteoclast cell number ( J:65766 )

• absent

abnormal bone marrow cavity morphology ( J:65766 )

• absent

osteopetrosis ( J:65766 )

behavior/neurological

behavior/neurological phenotype ( J:109459 )

N • genetic deficiency of Fos does not impair development of long-term memory in aversive taste learning; acute suppression of Fos expression does cause impairment

growth/size/body

failure of tooth eruption ( J:65766 )

decreased body size ( J:65766 )

• at 6 weeks

decreased fetal size ( J:65766 )

• mice are small at birth

vision/eye

decreased retina apoptosis ( J:65766 )

• mice exhibit resistance to light-induced retinal cell apoptosis unlike wild-type mice

craniofacial

craniofacial phenotype ( J:118753 )

N • when mice are transplanted with Nfatc1^tm1Mak, tooth eruption is observed

failure of tooth eruption ( J:65766 )

hematopoietic system

decreased osteoclast cell number ( J:65766 )

• absent

immune system

decreased osteoclast cell number ( J:65766 )

• absent

cellular

decreased retina apoptosis ( J:65766 )

• mice exhibit resistance to light-induced retinal cell apoptosis unlike wild-type mice

Genotype
MGI:3700975

hm2

• Allelic Composition: Fos^tm1Wag/Fos^tm1Wag
• Genetic Background: involves: 129S2/SvPas * C57BL/6

growth/size/body

failure of tooth eruption ( J:3493 )

tooth impaction ( J:3493 )

• incisors and molars are present but obstructed by abnormal amounts of bone

absent teeth ( J:3493 )

• mutants lack teeth

short snout ( J:3493 )

decreased body weight ( J:3493 )

• 10 days after birth, body weight is reduced ~40-60% compared to wild-type

limbs/digits/tail

short limbs ( J:3493 )

• homozygotes have much shorter limbs than littermates

skeleton

failure of tooth eruption ( J:3493 )

tooth impaction ( J:3493 )

• incisors and molars are present but obstructed by abnormal amounts of bone

absent teeth ( J:3493 )

• mutants lack teeth

abnormal long bone epiphyseal plate proliferative zone ( J:3493 )

• longitudinal columns of proliferative zone are irregular; zone of proliferating chondrocytes is greatly reduced

• this is observed in all bones examined

abnormal long bone hypertrophic chondrocyte zone ( J:3493 )

• zone of hypertrophic chondrocytes is increased

• this is observed in all bones examined

abnormal long bone epiphysis morphology ( J:3493 )

• much broader than wild-type

abnormal long bone metaphysis morphology ( J:3493 )

• much broader than wild-type

abnormal bone marrow cavity morphology ( J:3493 )

• bone cavities of long bone appear to be calcified

• cavities are occupied by abundant bone and cartilage trabeculae which reduce the effective bone marrow cavity by ~80%

abnormal compact bone morphology ( J:3493 )

• osteoblastic cells are absent along both the periosteal and endosteal cortical surfaces

abnormal compact bone thickness ( J:3493 )

• cortical bone is extremely thin and underdeveloped

increased bone trabecula number ( J:3493 )

• cavities are occupied by abundant bone and cartilage trabeculae which reduce the effective bone marrow cavity by ~80%

osteopetrosis ( J:3493 )

• this is the predominant phenotype in mutant mice

craniofacial

failure of tooth eruption ( J:3493 )

tooth impaction ( J:3493 )

• incisors and molars are present but obstructed by abnormal amounts of bone

absent teeth ( J:3493 )

• mutants lack teeth

short snout ( J:3493 )

hematopoietic system

abnormal thymus size ( J:3493 )

• in 4-6 week old mice, size of thymus is reduced

decreased thymocyte number ( J:3493 )

• total number of thymocytes is reduced by 90%

decreased double-positive T cell number ( J:3493 )

• frequency of double positive immature thymocytes is decreased >90% in 4-6 week-old mice

decreased B cell number ( J:3493 )

• there is a 75% reduction in B220+ B cells in the spleen in 4-6 week-old mice

increased T cell number ( J:3493 )

• in thymus there is 6-7-fold increase in CD3+ T cells bearing CD4+ or CD8+ in 4-6 week-old mice

abnormal spleen morphology ( J:3493 )

• mutant spleens have a poorly organized cortex and medulla

abnormal spleen white pulp morphology ( J:3493 )

• spleens show less dense white pulp in 4-6 week-old mice in 4-6 week-old mice

decreased splenocyte number ( J:3493 )

• total number of splenocytes is reduced by 25%

abnormal macrophage physiology ( J:118693 )

• macrophages show enhanced NF kappaB phosphorylation after Salmonella enterica serovar Typhimurium infection than wild-type cells

increased macrophage apoptosis ( J:118693 )

• macrophages show increased apoptotic death after Salmonella enterica serovar Typhimurium infection than wild-type cells

increased macrophage cytokine production ( J:118693 )

• macrophages show enhanced proinflammatory cytokine production after Salmonella enterica serovar Typhimurium infection than wild-type cells

immune system

abnormal thymus size ( J:3493 )

• in 4-6 week old mice, size of thymus is reduced

decreased thymocyte number ( J:3493 )

• total number of thymocytes is reduced by 90%

decreased double-positive T cell number ( J:3493 )

• frequency of double positive immature thymocytes is decreased >90% in 4-6 week-old mice

decreased B cell number ( J:3493 )

• there is a 75% reduction in B220+ B cells in the spleen in 4-6 week-old mice

increased T cell number ( J:3493 )

• in thymus there is 6-7-fold increase in CD3+ T cells bearing CD4+ or CD8+ in 4-6 week-old mice

abnormal spleen morphology ( J:3493 )

• mutant spleens have a poorly organized cortex and medulla

abnormal spleen white pulp morphology ( J:3493 )

• spleens show less dense white pulp in 4-6 week-old mice in 4-6 week-old mice

decreased splenocyte number ( J:3493 )

• total number of splenocytes is reduced by 25%

abnormal macrophage physiology ( J:118693 )

• macrophages show enhanced NF kappaB phosphorylation after Salmonella enterica serovar Typhimurium infection than wild-type cells

increased macrophage apoptosis ( J:118693 )

• macrophages show increased apoptotic death after Salmonella enterica serovar Typhimurium infection than wild-type cells

increased macrophage cytokine production ( J:118693 )

• macrophages show enhanced proinflammatory cytokine production after Salmonella enterica serovar Typhimurium infection than wild-type cells

abnormal cytokine secretion ( J:118693 )

• mutant macrophages show enhanced production of proinflammatory cytokines after Salmonella enterica serovar Typhimurium infection

increased susceptibility to bacterial infection ( J:118693 )

• Fos-deficient mice are more susceptible to Salmonella infection

reproductive system

transmission ratio distortion ( J:3493 )

• there is distorted female transmission frequency; heterozygous males mated to wild-type produce 51% heterozygous offspring, while heterozygous females mated to wild-type males produce only 34% heterozygous pups

cellular

increased macrophage apoptosis ( J:118693 )

• macrophages show increased apoptotic death after Salmonella enterica serovar Typhimurium infection than wild-type cells

endocrine/exocrine glands

abnormal thymus size ( J:3493 )

• in 4-6 week old mice, size of thymus is reduced

decreased thymocyte number ( J:3493 )

• total number of thymocytes is reduced by 90%

Genotype
MGI:3850715

ht3

• Allelic Composition: Fos^tm1Wag/Fos^{tm2(Fosl1)Wag}
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6

skeleton

failure of tooth eruption ( J:65766 )

absent teeth ( J:65766 )

decreased osteoclast cell number ( J:65766 )

• absent

abnormal bone marrow cavity morphology ( J:65766 )

• absent

osteopetrosis ( J:65766 )

vision/eye

increased retina apoptosis ( J:65766 )

• mice exhibit increased light-induced retinal cell apoptosis compared with wild-type mice

craniofacial

failure of tooth eruption ( J:65766 )

absent teeth ( J:65766 )

growth/size/body

failure of tooth eruption ( J:65766 )

absent teeth ( J:65766 )

decreased body size ( J:65766 )

• at 6 weeks

immune system

decreased osteoclast cell number ( J:65766 )

• absent

hematopoietic system

decreased osteoclast cell number ( J:65766 )

• absent

cellular

increased retina apoptosis ( J:65766 )

• mice exhibit increased light-induced retinal cell apoptosis compared with wild-type mice

Genotype
MGI:3700989

cx4

• Allelic Composition: Fos^tm1Wag/Fos^tm1Wag; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129S2/SvPas * C57BL/6

skeleton

osteopetrosis ( J:89283 )

• double mutants display similar osteopetrotic phenotype to Fos^tm1Wag mice

neoplasm

increased rhabdomyosarcoma incidence ( J:89283 )

• at 10 weeks of age, double mutants develop facial and orbital tumors with penetrance of 93% at 25 weeks of age

• tumors are polygonal or elongated with pleomorphic nuclei and eosinophilic cytoplasm with cross-striations

• tumors display invasive growth into facial and external orbital muscles

muscle

increased rhabdomyosarcoma incidence ( J:89283 )

• at 10 weeks of age, double mutants develop facial and orbital tumors with penetrance of 93% at 25 weeks of age

• tumors are polygonal or elongated with pleomorphic nuclei and eosinophilic cytoplasm with cross-striations

• tumors display invasive growth into facial and external orbital muscles

Genotype
MGI:3700990

cx5

• Allelic Composition: Fos^tm1Wag/Fos^tm1Wag; Trp53^tm1Tyj/Trp53⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6

neoplasm

increased rhabdomyosarcoma incidence ( J:89283 )

• double mutants develop facial and orbital tumors with lower penetrance than in double homozygous mice

muscle

increased rhabdomyosarcoma incidence ( J:89283 )

• double mutants develop facial and orbital tumors with lower penetrance than in double homozygous mice