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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Ins2tm1Jja
targeted mutation 1, Jacques Jami
MGI:2181878
Summary 10 genotypes


Genotype
MGI:3584435
hm1
Allelic
Composition		 Ins2tm1Jja/Ins2tm1Jja
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ins2tm1Jja mutation (4 available); any Ins2 mutation (93 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
endocrine/exocrine glands
increased pancreatic beta cell mass ( J:77595 )
• beta cell mass almost 3X more than controls more than in wild-type controls
• cell size normal
increased insulin secretion ( J:77595 )
• insulin content of islets is almost 2X that of controls
• however, plasma insulin levels are normal

homeostasis/metabolism
increased insulin secretion ( J:77595 )
• insulin content of islets is almost 2X that of controls
• however, plasma insulin levels are normal




Genotype
MGI:3716137
hm2
Allelic
Composition		 Ins2tm1Jja/Ins2tm1Jja
Genetic
Background		 NOD.129S2-Ins2tm1Jja
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ins2tm1Jja mutation (4 available); any Ins2 mutation (93 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Severe insulitis in Ins2tm1Jja/Ins2tm1Jja mice and lack of insulitis in Ins1tm1Jja/Ins1tm1Jja mice

homeostasis/metabolism
abnormal glucose homeostasis ( J:85309 )
• development of diabetes is accelerated compared to wild-type controls; all but one female develop diabetes between 7 and 15 weeks of age

immune system
insulitis ( J:85309 )
• severe insulitis with primarily intraislet infiltrates
increased anti-insulin autoantibody level ( J:85309 )
• both males and females have higher peak levels of insulin autoantibodies than wild-type

endocrine/exocrine glands
insulitis ( J:85309 )
• severe insulitis with primarily intraislet infiltrates

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus DOID:9744 OMIM:222100
 J:85309




Genotype
MGI:3521711
ht3
Allelic
Composition		 Ins2tm1Delt/Ins2tm1Jja
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ins2tm1Delt mutation (0 available); any Ins2 mutation (93 available)
Ins2tm1Jja mutation (4 available); any Ins2 mutation (93 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
endocrine/exocrine glands
decreased pancreatic beta cell number ( J:94589 )
• no beta cells are detected in the pancreas




Genotype
MGI:3584436
cx4
Allelic
Composition		 Ins1tm1Jja/Ins1tm1Jja
Ins2tm1Jja/Ins2tm1Jja
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ins1tm1Jja mutation (5 available); any Ins1 mutation (46 available)
Ins2tm1Jja mutation (4 available); any Ins2 mutation (93 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Growth retardation of Ins1tm1Jja/Ins1tm1Jja Ins2tm1Jja/Ins2tm1Jja mice

mortality/aging
postnatal lethality, complete penetrance ( J:40377 )
• death in 48 hours after birth on average

endocrine/exocrine glands
increased pancreatic beta cell number ( J:77595 )
• significant hyperplasia
enlarged pancreatic islets ( J:40377 )
• about 1.5X larger than in littermate controls

homeostasis/metabolism
abnormal glucose homeostasis ( J:40377 , J:77595 )
• severely diabetic (J:77595)
increased urine glucose level ( J:40377 )
• glycosuria detectable after pups suckle
acidosis ( J:40377 )
• ketoacidosis

growth/size/body
embryonic growth retardation ( J:40377 )
decreased body size ( J:40377 )
• mean body weight within a few hours of birth 22% less than littermate controls
enlarged liver ( J:40377 )

liver/biliary system

renal/urinary system
increased urine glucose level ( J:40377 )
• glycosuria detectable after pups suckle

embryo

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
maturity-onset diabetes of the young DOID:0050524 OMIM:606391
 J:40377 , J:77595




Genotype
MGI:3687167
cx5
Allelic
Composition		 H2g7/H2g7
Ins2tm1Jja/Ins2tm1Jja
Rag1tm1Mom/Rag1tm1Mom
Tg(TcraBDC12-4.1)10Jos/0
Tg(TcrbBDC12-4.1)82Gse/0
Genetic
Background		 involves: 129S2/SvPas * 129S7/SvEvBrd * C57BL/6 * FVB * NOD
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2g7 mutation (24 available); any H2 mutation (281 available)
Ins2tm1Jja mutation (4 available); any Ins2 mutation (93 available)
Rag1tm1Mom mutation (49 available); any Rag1 mutation (123 available)
Tg(TcraBDC12-4.1)10Jos mutation (2 available)
Tg(TcrbBDC12-4.1)82Gse mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
homeostasis/metabolism

immune system
increased susceptibility to autoimmune diabetes ( J:111874 )
• mice develop diabetes (blood glucose >250 ml/dl) more rapidly than transgenic Rag1-null, Ins2-sufficient mice

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus DOID:9744 OMIM:222100
 J:111874




Genotype
MGI:3640380
cx6
Allelic
Composition		 Ins1tm1Jja/Ins1tm1Jja
Ins2tm1Jja/Ins2tm1Jja
Genetic
Background		 involves: 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ins1tm1Jja mutation (5 available); any Ins1 mutation (46 available)
Ins2tm1Jja mutation (4 available); any Ins2 mutation (93 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
growth/size/body
decreased birth weight ( J:106827 )
• at birth the relative reduction in body weight is 22%
decreased fetal weight ( J:106827 )
• at E18.5, body weight is reduced by 15% compared to heterozygous pups

cellular
decreased pancreatic islet cell apoptosis ( J:106827 )
• there is decreased islet cell apoptosis in in all regions of the islets compared to controls
increased pancreatic beta cell proliferation ( J:106827 )
• at E18.5 there is increased islet cell proliferation compared to controls, with both alpha and beta cells proliferating
increased pancreatic alpha cell proliferation ( J:106827 )
• at E18.5 there is increased islet cell proliferation compared to controls, with both alpha and beta cells proliferating

endocrine/exocrine glands
decreased pancreatic islet cell apoptosis ( J:106827 )
• there is decreased islet cell apoptosis in in all regions of the islets compared to controls
increased pancreatic beta cell proliferation ( J:106827 )
• at E18.5 there is increased islet cell proliferation compared to controls, with both alpha and beta cells proliferating
increased pancreatic alpha cell proliferation ( J:106827 )
• at E18.5 there is increased islet cell proliferation compared to controls, with both alpha and beta cells proliferating
increased pancreatic alpha cell mass ( J:106827 )
• total alpha cell mass is increased compared to wild-type and Ins1-null, Ins2-heterozygous controls (38 ug w.t. vs 45 ug vs 68 ug double nulls)
increased pancreatic beta cell mass ( J:106827 )
• total beta cell mass at E18.5, is 348 ug compared to 226 ug in Ins1-null, Ins2-heterozygous controls
enlarged pancreatic islets ( J:106827 )
• mean islet area is significantly increased compared with Ins1-null, Ins2-heterozygous controls at E18.5 and P1 but not at E17.5
• compared to wild-type controls, mice have more large islets and fewer small islets




Genotype
MGI:3640381
cx7
Allelic
Composition		 Ins1tm1Jja/Ins1tm1Jja
Ins2tm1Jja/Ins2+
Genetic
Background		 involves: 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ins1tm1Jja mutation (5 available); any Ins1 mutation (46 available)
Ins2tm1Jja mutation (4 available); any Ins2 mutation (93 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
endocrine/exocrine glands
increased pancreatic alpha cell mass ( J:106827 )
• total alpha cell mass is increased compared to wild-type (68 ug vs 48 ug w.t.)




Genotype
MGI:3619380
cx8
Allelic
Composition		 Ins1tm1Jja/Ins1tm1Jja
Ins2tm1Jja/Ins2tm1Jja
Tg(Ins2*Y16A)1Ell/0
Genetic
Background		 NOD.Cg-Ins1tm1Jja Ins2tm1Jja Tg(Ins2*Y16A)1Ell
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ins1tm1Jja mutation (5 available); any Ins1 mutation (46 available)
Ins2tm1Jja mutation (4 available); any Ins2 mutation (93 available)
Tg(Ins2*Y16A)1Ell mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
homeostasis/metabolism
increased circulating glucose level ( J:98583 )
• male transgenic mice lacking both native insulin genes develop diabetes before 10 weeks of age (2 consecutive measurements of >250 mg/dl glucose)

immune system
salivary gland inflammation ( J:98583 )
• at 26 weeks of age, sialitis is observed but not insulitis
decreased susceptibility to autoimmune diabetes ( J:98583 )
• female transgenic mice lacking both native insulin genes do not exhibit diabetes by 30 weeks of age

endocrine/exocrine glands
salivary gland inflammation ( J:98583 )
• at 26 weeks of age, sialitis is observed but not insulitis

digestive/alimentary system
salivary gland inflammation ( J:98583 )
• at 26 weeks of age, sialitis is observed but not insulitis

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
NOT type 1 diabetes mellitus DOID:9744 OMIM:222100
 J:98583




Genotype
MGI:3619054
cx9
Allelic
Composition		 Ins1tm1Jja/Ins1tm1Jja
Ins2tm1Jja/Ins2tm1Jja
Tg(Ins2*Y16A)3Ell/0
Genetic
Background		 NOD.Cg-Ins1tm1Jja Ins2tm1Jja Tg(Ins2*Y16A)3Ell
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ins1tm1Jja mutation (5 available); any Ins1 mutation (46 available)
Ins2tm1Jja mutation (4 available); any Ins2 mutation (93 available)
Tg(Ins2*Y16A)3Ell mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
salivary gland inflammation ( J:98583 )
• at 26 weeks of age, sialitis is observed but not insulitis
decreased susceptibility to autoimmune diabetes ( J:98583 )
• no female double knockout mice carrying the transgene on a NOD background develop diabetes over 30 weeks of observation
decreased anti-insulin autoantibody level ( J:98583 )
• females lacking both insulin genes do not produce insulin autoantibodies

digestive/alimentary system
salivary gland inflammation ( J:98583 )
• at 26 weeks of age, sialitis is observed but not insulitis

endocrine/exocrine glands
salivary gland inflammation ( J:98583 )
• at 26 weeks of age, sialitis is observed but not insulitis

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
NOT type 1 diabetes mellitus DOID:9744 OMIM:222100
 J:98583




Genotype
MGI:3619056
cx10
Allelic
Composition		 Ins2tm1Jja/Ins2tm1Jja
Tg(Ins2*Y16A)3Ell/0
Genetic
Background		 NOD.Cg-Ins2tm1Jja Tg(Ins2*Y16A)3Ell
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ins2tm1Jja mutation (4 available); any Ins2 mutation (93 available)
Tg(Ins2*Y16A)3Ell mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
autoimmune response ( J:98583 )
• transgenic female mice lacking the Ins2 gene do not show increased resistance to diabetes development compared to nontransgenic NOD animals lacking the gene
increased anti-insulin autoantibody level ( J:98583 )
• female mice having the insulin 1 gene but lacking the insulin 2 gene produce insulin autoantibodies




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Send questions and comments to User Support. 		last database update
10/09/2024
MGI 6.24 		The Jackson Laboratory