Phenotypes associated with this allele

• Allele Symbol: Tg(Fabp4-cre)1Abel
• Allele Name: transgene insertion 1, E Dale Abel
• Allele ID: MGI:2182103
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pten^tm1Hwu/Pten^tm1Hwu Tg(Fabp4-cre)1Abel/0	involves: 129S4/SvJae	MGI:4830361
cn2	Slc2a4^tm1Abel/Slc2a4^tm1Abel Tg(Fabp4-cre)1Abel/?	involves: 129S4/SvJae * FVB	MGI:3029364
cn3	Igf1r^tm1Jcbr/Igf1r^tm1Jcbr Tg(Fabp4-cre)1Abel/?	involves: C57BL/6 * FVB/N	MGI:3818455
cn4	Gnas^tm5.1Lsw/Gnas^tm5.1Lsw Tg(Fabp4-cre)1Abel/0	involves: FVB	MGI:6102945

Genotype
MGI:4830361

cn1

• Allelic Composition: Pten^tm1Hwu/Pten^tm1Hwu; Tg(Fabp4-cre)1Abel/0
• Genetic Background: involves: 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

adipose tissue phenotype ( J:97588 )

N • no differences in body weight and fat storage

homeostasis/metabolism

decreased circulating insulin level ( J:97588 )

• fasting mutants exhibit a 2.1-fold decrease in plasma insulin levels compared to controls indicating enhanced insulin sensitivity

improved glucose tolerance ( J:97588 )

• during a glucose tolerance test, mutants show a blunted elevation of blood glucose; the mean peak increase in blood glucose is 19% lower than that of controls

• mutants are protected from developing streptozotocin-induced diabetes; they maintain normal glycemia and remain resistant to the development of hyperglycemia

increased insulin sensitivity ( J:97588 )

• mutants are more sensitive to insulin challenge (in insulin tolerance test), showing a 24% lower blood glucose at 60 min compared to wild-type mice

• insulin resistance is 1.9-fold lower for mutants than controls

abnormal cytokine level ( J:97588 )

• 36% lower serum concentrations of the adipocytokine resistin

immune system

abnormal cytokine level ( J:97588 )

• 36% lower serum concentrations of the adipocytokine resistin

Genotype
MGI:3029364

cn2

• Allelic Composition: Slc2a4^tm1Abel/Slc2a4^tm1Abel; Tg(Fabp4-cre)1Abel/?
• Genetic Background: involves: 129S4/SvJae * FVB

homeostasis/metabolism

abnormal glucose homeostasis ( J:67292 )

• basal glucose uptake reduced about 40% in adipocytes

• insulin mediated glucose uptake was blunted to a maximum of about 72%

• whole body glucose uptake reduced 53%

• glycolysis down 50%

• transport in to white and brown adipose tissue and skeletal muscle reduced

• insulin unable to suppress hepatic glucose production

abnormal glucose tolerance ( J:67292 )

• impaired glucose tolerance as early as 13 weeks and persisting until after 31 weeks of age

abnormal glycogen homeostasis ( J:67292 )

• glycogen synthesis down 67%

insulin resistance ( J:67292 )

• as early as 12 weeks and sometimes becomes extreme

Genotype
MGI:3818455

cn3

• Allelic Composition: Igf1r^tm1Jcbr/Igf1r^tm1Jcbr; Tg(Fabp4-cre)1Abel/?
• Genetic Background: involves: C57BL/6 * FVB/N

growth/size/body

increased heart weight ( J:141321 )

• disproportionately increased at 24 weeks

increased body weight ( J:141321 )

• both males and females have gained more weight than controls by 10 weeks of age

• daily food intake normal

increased body length ( J:141321 )

• significantly increased naso-anal length by 24-32 weeks of age

increased liver weight ( J:141321 )

• disproportionately increased at 24 weeks

adipose tissue

increased total body fat amount ( J:141321 )

• disproportionately increased in weight at 24 weeks

abnormal fat cell morphology ( J:141321 )

• increased number and diameter of adipocytes

increased fat cell size ( J:141321 )

abnormal gonadal fat pad morphology ( J:141321 )

• adipocyte abnormalities particularly evident in epigonadal fat

liver/biliary system

increased liver weight ( J:141321 )

• disproportionately increased at 24 weeks

cardiovascular system

increased heart weight ( J:141321 )

• disproportionately increased at 24 weeks

nervous system

decreased brain size ( J:141321 )

• relative brain weight is significantly reduced at 24 weeks

homeostasis/metabolism

abnormal glucose homeostasis ( J:141321 )

increased circulating glucose level ( J:141321 )

• fasted blood glucose significantly increased at 12 weeks but not 24 weeks of age

• fed blood glucose levels in males also increased at 12 weeks

increased insulin sensitivity ( J:141321 )

• of isolated adipocytes

decreased adiponectin level ( J:141321 )

• significantly lower at 12 weeks

Genotype
MGI:6102945

cn4

• Allelic Composition: Gnas^tm5.1Lsw/Gnas^tm5.1Lsw; Tg(Fabp4-cre)1Abel/0
• Genetic Background: involves: FVB

muscle

decreased skeletal muscle triglyceride level ( J:159612 )

mortality/aging

premature death ( J:159612 )

• by 14 weeks in severely affected mice

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:159612 )

N • surviving mice exhibit normal food intake, energy expenditure and respiratory ratio at ambient or cold temperatures

N • mice exhibit normal T4 levels

increased fatty acid oxidation ( J:159612 )

• in the muscle of mice fed a high-fat diet

impaired adaptive thermogenesis ( J:159612 )

• in response to cold exposure on a high fat diet, mice exhibit reduced body temperature and a 3-fold increase in energy expenditure compared with control mice

hypoglycemia ( J:159612 )

• under basal fed conditions

increased circulating corticosterone level ( J:159612 )

decreased circulating insulin level ( J:159612 )

• under basal fed conditions

decreased circulating leptin level ( J:159612 )

• at 3 months in surviving mice

• however, adiponectin levels are normal

decreased circulating free fatty acids level ( J:159612 )

decreased circulating triglyceride level ( J:159612 )

abnormal urine catecholamine level ( J:159612 )

• increased norephinephrine excretion

increased energy expenditure ( J:159612 )

• 3-fold in response to cold exposure on a high fat diet

decreased susceptibility to diet-induced obesity ( J:159612 )

• when fed a high-fat diet

improved glucose tolerance ( J:159612 )

increased insulin sensitivity ( J:159612 )

decreased liver triglyceride level ( J:159612 )

decreased skeletal muscle triglyceride level ( J:159612 )

adipose tissue

decreased subcutaneous adipose tissue amount ( J:159612 )

• almost completely absent in severely affected mice

decreased white adipose tissue amount ( J:159612 )

• at 3 months in surviving mice

• however, no macrophage infiltration is observed

abnormal adipose tissue development ( J:159612 )

• embryonic cells exhibit reduced adipogenesis compared with control cells

decreased body fat mass ( J:159612 )

• at 3 months in surviving mice

• however, weights of brown adipose tissue, liver, kidney and heart are normal

decreased white fat cell size ( J:159612 )

• in epididymal WAT

decreased epididymal fat pad weight ( J:159612 )

• almost completely absent in severely affected mice

decreased inguinal fat pad weight ( J:159612 )

• almost completely absent in severely affected mice

decreased mesenteric fat pad weight ( J:159612 )

• almost completely absent in severely affected mice

decreased renal fat pad weight ( J:159612 )

• almost completely absent in severely affected mice

abnormal white adipose tissue physiology ( J:159612 )

• isoproterenol-treated white adipose tissue adipocytes exhibit reduced lipolytic response compared with control cells

growth/size/body

decreased body fat mass ( J:159612 )

• at 3 months in surviving mice

• however, weights of brown adipose tissue, liver, kidney and heart are normal

increased lean body mass ( J:159612 )

• at 3 months in surviving mice

decreased susceptibility to diet-induced obesity ( J:159612 )

• when fed a high-fat diet

decreased body size ( J:159612 )

• in severely affected mice

decreased body weight ( J:159612 )

• at 3 months in surviving mice

• however, weights of brown adipose tissue, liver, kidney and heart are normal

decreased body length ( J:159612 )

• at 3 months in surviving mice

behavior/neurological

decreased locomotor activity ( J:159612 )

• in cold-exposed mice

liver/biliary system

decreased liver triglyceride level ( J:159612 )

renal/urinary system

abnormal urine catecholamine level ( J:159612 )

• increased norephinephrine excretion

integument

decreased subcutaneous adipose tissue amount ( J:159612 )

• almost completely absent in severely affected mice

cellular

increased fatty acid oxidation ( J:159612 )

• in the muscle of mice fed a high-fat diet