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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Ddx4tm1Tnc
targeted mutation 1, Toshiaki Noce
MGI:2182568
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Ddx4tm1Tnc/Ddx4tm1Tnc involves: 129P2/OlaHsd * C57BL/6 MGI:3692739
hm2
 		Ddx4tm1Tnc/Ddx4tm1Tnc involves: 129P2/OlaHsd * C57BL/6NJcl MGI:2660566
ht3
 		Ddx4tm1Tnc/Ddx4+ involves: 129P2/OlaHsd * C57BL/6NJcl MGI:2660567


Genotype
MGI:3692739
hm1
Allelic
Composition		 Ddx4tm1Tnc/Ddx4tm1Tnc
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ddx4tm1Tnc mutation (0 available); any Ddx4 mutation (59 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal oocyte morphology ( J:115333 )
• intermitochondrial cement is markedly reduced in mutant oocytes
abnormal spermatocyte morphology ( J:115333 )
• intermitochondrial cement is lacking in surviving spermatocytes although clusters of mitochondria have formed and is seen at reduced levels in prospermatogonia compared to wild-type
arrest of male meiosis ( J:115333 )
• spermatogenesis ceases at prophase of meiosis; most spermatocytes at this stage are undergoing degeneration; round spermatids are not present in testes

cellular
abnormal oocyte morphology ( J:115333 )
• intermitochondrial cement is markedly reduced in mutant oocytes
abnormal spermatocyte morphology ( J:115333 )
• intermitochondrial cement is lacking in surviving spermatocytes although clusters of mitochondria have formed and is seen at reduced levels in prospermatogonia compared to wild-type
arrest of male meiosis ( J:115333 )
• spermatogenesis ceases at prophase of meiosis; most spermatocytes at this stage are undergoing degeneration; round spermatids are not present in testes




Genotype
MGI:2660566
hm2
Allelic
Composition		 Ddx4tm1Tnc/Ddx4tm1Tnc
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6NJcl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ddx4tm1Tnc mutation (0 available); any Ddx4 mutation (59 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
endocrine/exocrine glands
small seminiferous tubules ( J:61665 )
• reduced size apparent by 2 days of age
• vas deferens and epididymis were grossly normal
testicular atrophy ( J:61665 )
• adult testes atrophied to approximately 1/5 the size of adult wild-type and heterozygous testes

reproductive system
decreased primordial germ cell number ( J:61665 )
• reduced number of primordial germ cells (PGCs) in male gonads at 12.5 dpc due to impaired proliferation
• number of PGCs in female gonads at 12.5 dpc was comparable to wild-type
arrest of male meiosis ( J:61665 )
• arrest during prophase around the leptotene to zygotene stages
increased male germ cell apoptosis ( J:61665 )
• ~10-fold increase in TUNEL+ cells in the seminiferous tubules, indicating that arrested germ cells undergo apoptotic cell death
abnormal primordial germ cell migration ( J:61665 )
• ectopic PGCs were observed outside of the testis cord
decreased primordial germ cell proliferation ( J:61665 )
• reduced number of primordial germ cells (PGCs) in male gonads at 12.5 dpc due to impaired proliferation
small seminiferous tubules ( J:61665 )
• reduced size apparent by 2 days of age
• vas deferens and epididymis were grossly normal
testicular atrophy ( J:61665 )
• adult testes atrophied to approximately 1/5 the size of adult wild-type and heterozygous testes
male infertility ( J:61665 )
• males were infertile; however, males and females exhibited normal sexual behavior

cellular
decreased primordial germ cell number ( J:61665 )
• reduced number of primordial germ cells (PGCs) in male gonads at 12.5 dpc due to impaired proliferation
• number of PGCs in female gonads at 12.5 dpc was comparable to wild-type
arrest of male meiosis ( J:61665 )
• arrest during prophase around the leptotene to zygotene stages
increased male germ cell apoptosis ( J:61665 )
• ~10-fold increase in TUNEL+ cells in the seminiferous tubules, indicating that arrested germ cells undergo apoptotic cell death
abnormal primordial germ cell migration ( J:61665 )
• ectopic PGCs were observed outside of the testis cord
decreased primordial germ cell proliferation ( J:61665 )
• reduced number of primordial germ cells (PGCs) in male gonads at 12.5 dpc due to impaired proliferation




Genotype
MGI:2660567
ht3
Allelic
Composition		 Ddx4tm1Tnc/Ddx4+
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6NJcl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ddx4tm1Tnc mutation (0 available); any Ddx4 mutation (59 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
decreased primordial germ cell number ( J:61665 )
• reduced number of primordial germ cells (PGCs) in male gonads at 12.5 dpc
• however, males and females are fertile
abnormal primordial germ cell migration ( J:61665 )
• ectopic PGCs were observed outside of the testis cord

cellular
decreased primordial germ cell number ( J:61665 )
• reduced number of primordial germ cells (PGCs) in male gonads at 12.5 dpc
• however, males and females are fertile
abnormal primordial germ cell migration ( J:61665 )
• ectopic PGCs were observed outside of the testis cord




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory