Analysis Tools
endocrine/exocrine glands
|
• zymogen granule rare in embryos but become more abundant after birth
|
|
• acinar cell disruptions similar to those of the pancreas are seen
|
|
• morphology of the chief cells is disrupted
|
 |
• although initial development of the seminal vesicle is normal, mice exhibit impaired differentiation of cell morphology and function of the secretory epithelium leading to degeneration of the seminal vesicle
|
|
|
• at 2 months of age, mice display abnormal cell morphology in seminal vesicle epithelium, as shown by altered Toluidine blue staining intensity either of the nuclei or cytoplasm
• at high magnification, epithelial secretory cells show reduced ruffling of the luminal border and atypical granule localization
|
|
|
• at 2 months of age, TEM analysis indicates decreased granule size, abnormal (electron dense) granules as well as decreased microvilli and distended ER suggestive of reduced exocytosis ability
• at 12 months of age, mice show progressive deterioration and degeneration of the seminal vesicle epithelium, with numerous abnormal intracellular granules, altered nuclear architecture (indented border and electron dense appearance), and significant accumulations of autophagic bodies
|
digestive/alimentary system
|
• zymogen granule rare in embryos but become more abundant after birth
|
|
• acinar cell disruptions similar to those of the pancreas are seen
|
|
• morphology of the chief cells is disrupted
|
|
• glandular cells are more cuboidal than columnar in form
|
reproductive system
|
N |
• despite alterations in seminal fluid composition, male reproductive function and fertility are normal, as determined by the number of identified copulatory plugs, number of litters generated, and number of offspring per litter
|
|
|
• although initial development of the seminal vesicle is normal, mice exhibit impaired differentiation of cell morphology and function of the secretory epithelium leading to degeneration of the seminal vesicle
|
|
|
• at 2 months of age, mice display abnormal cell morphology in seminal vesicle epithelium, as shown by altered Toluidine blue staining intensity either of the nuclei or cytoplasm
• at high magnification, epithelial secretory cells show reduced ruffling of the luminal border and atypical granule localization
|
|
|
• at 2 months of age, TEM analysis indicates decreased granule size, abnormal (electron dense) granules as well as decreased microvilli and distended ER suggestive of reduced exocytosis ability
• at 12 months of age, mice show progressive deterioration and degeneration of the seminal vesicle epithelium, with numerous abnormal intracellular granules, altered nuclear architecture (indented border and electron dense appearance), and significant accumulations of autophagic bodies
|
|
|
• at 2 months of age, no clear zone between the seminal fluid and epithelial lining is observed, suggesting altered seminal fluid composition and decreased viscosity
• at 10 months of age, seminal fluid shows increased accumulation of 1,4-alpha-D-glucan glucanohydrolase (53 kDa; salivary and hepatic precursor for alpha-amylase), seminal vesicle secretory protein 2 (semenoclotin; 40 kDa), and carcinoembryonic antigen (CEA)-related cell adhesion molecule 10 (CEACAM10; 37 kDa), as shown by mass spectrometry analysis
• conversely, accumulation of seminal vesicle secretory (SVS) proteins IV and V (25 kDa) is reduced relative to wild-type controls
|
