Phenotypes associated with this allele

• Allele Symbol: Nfatc2^tm1Srf
• Allele Name: targeted mutation 1, Edgar Serfling
• Allele ID: MGI:2182770
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nfatc2^tm1Srf/Nfatc2^tm1Srf	B6.Cg-Nfatc2^tm1Srf	MGI:3849012
hm2	Nfatc2^tm1Srf/Nfatc2^tm1Srf	involves: 129P2/OlaHsd	MGI:3849011
hm3	Nfatc2^tm1Srf/Nfatc2^tm1Srf	involves: 129P2/OlaHsd * C57BL/6	MGI:3654100

Genotype
MGI:3849012

hm1

• Allelic Composition: Nfatc2^tm1Srf/Nfatc2^tm1Srf
• Genetic Background: B6.Cg-Nfatc2^tm1Srf

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased immunoglobulin level ( J:86279 )

increased IgE level ( J:86279 )

• as a result of infection with either Nippostrongylus brasiliensis or Mycobacterium bovis

increased IgG1 level ( J:86279 )

• as a result of Nippostrongylus brasiliensis infection

abnormal CD4-positive, alpha-beta T cell physiology ( J:86279 )

abnormal T-helper 1 physiology ( J:86279 )

• Th1 responses are decreased overall

abnormal T-helper 2 physiology ( J:86279 )

• Th2 responses are increased overall

decreased interferon-gamma secretion ( J:86279 )

• Mycobacterium bovis infection results in lowered Ifn gamma secretion

increased interleukin-4 secretion ( J:86279 )

• Nippostrongylus brasiliensis infection leads to significantly increased Il4 and Il5 secretion

increased interleukin-5 secretion ( J:86279 )

• Nippostrongylus brasiliensis infection leads to significantly increased Il4 and Il5 secretion

hematopoietic system

increased immunoglobulin level ( J:86279 )

increased IgE level ( J:86279 )

• as a result of infection with either Nippostrongylus brasiliensis or Mycobacterium bovis

increased IgG1 level ( J:86279 )

• as a result of Nippostrongylus brasiliensis infection

abnormal CD4-positive, alpha-beta T cell physiology ( J:86279 )

abnormal T-helper 1 physiology ( J:86279 )

• Th1 responses are decreased overall

abnormal T-helper 2 physiology ( J:86279 )

• Th2 responses are increased overall

Genotype
MGI:3849011

hm2

• Allelic Composition: Nfatc2^tm1Srf/Nfatc2^tm1Srf
• Genetic Background: involves: 129P2/OlaHsd

immune system

increased lymphocyte cell number ( J:45252 )

• normal immune system at birth

• two fold increase in lymphoid cells after 4-6 months

increased B cell number ( J:45252 )

• 4 fold increase in lymph node B cells

increased T cell number ( J:45252 )

• 10 fold increase in T lymphocytes

abnormal immune system organ morphology ( J:45252 )

enlarged spleen ( J:45252 )

abnormal lymph node morphology ( J:45252 )

enlarged lymph nodes ( J:45252 )

lymph node hyperplasia ( J:45252 )

• 6 fold increase in lymph node cells

abnormal immune system physiology ( J:45252 )

abnormal T cell physiology ( J:45252 )

• increased longevity of Staphylococcal enterotoxin A stimulated T cells

abnormal interleukin secretion ( J:45252 )

• decreased synthesis of Il4 and Il5 in Staphylococcal enterotoxin A stimulated T cells

• reduced expression of IL2 receptor alpha after ConA stimulation

hematopoietic system

enlarged spleen ( J:45252 )

increased lymphocyte cell number ( J:45252 )

• normal immune system at birth

• two fold increase in lymphoid cells after 4-6 months

increased B cell number ( J:45252 )

• 4 fold increase in lymph node B cells

increased T cell number ( J:45252 )

• 10 fold increase in T lymphocytes

abnormal T cell physiology ( J:45252 )

• increased longevity of Staphylococcal enterotoxin A stimulated T cells

growth/size/body

enlarged spleen ( J:45252 )

Genotype
MGI:3654100

hm3

• Allelic Composition: Nfatc2^tm1Srf/Nfatc2^tm1Srf
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

enlarged thymus ( J:110686 )

thymus hyperplasia ( J:110686 )

• thymi of mice that are 6 months old show an increased cellularity compared to wild-type

abnormal thymus involution ( J:110686 )

• animals over 6 months of age show abnormal involution; thymi of mutants exhibit large cortical areas similar to young wild-type mice

enlarged spleen ( J:110686 )

• spleens of mutants are massively enlarged due to extended white pulp areas

abnormal B cell number ( J:110686 )

• 5% of cells are mature IgM+/IgD+ B lymphocytes in these B cell areas in the thymus

increased activated T cell number ( J:110686 )

• peripheral T cells in mutants older than 2 months display a preactivated phenotype; lymph node T cells express high levels of CD44 and CD69 compared to wild-type while 2 to 3-fold more T cells express low levels of CD62L

abnormal spleen germinal center morphology ( J:110686 )

• enlarged

abnormal lymphocyte physiology ( J:110686 )

• a normal proliferative response is mounted by lymph node T cells when exposed the superantigen staphylococcal enterotoxin (SEA) or increasing CD3-specific antibodies in vitro but a 3- to 4-fold increase in proliferation is detected in the secondary response after restimulation with antigen

abnormal B cell physiology ( J:110686 )

• 10% of mature B cells show an activated phenotype in the thymus

abnormal T cell physiology ( J:110686 )

• mutants exhibit a gradual increase in peripheral lymphocytes; up to 2 months of age, numbers are similar to wild-type but the numbers increase 2-fold and up to 5- to 6-fold in mice older than 6 months

• after in vivo stimulation with SEA for 8 days, CD4+Vb11+ T cells show normal expansion but distinctly retarded deletion compared to wild-type (incomplete antigen response termination)

abnormal lymph node B cell domain morphology ( J:110686 )

• a large increase in the size of germinal centers in the lymph nodes is observed

hematopoietic system

enlarged thymus ( J:110686 )

thymus hyperplasia ( J:110686 )

• thymi of mice that are 6 months old show an increased cellularity compared to wild-type

abnormal thymus involution ( J:110686 )

• animals over 6 months of age show abnormal involution; thymi of mutants exhibit large cortical areas similar to young wild-type mice

enlarged spleen ( J:110686 )

• spleens of mutants are massively enlarged due to extended white pulp areas

abnormal B cell number ( J:110686 )

• 5% of cells are mature IgM+/IgD+ B lymphocytes in these B cell areas in the thymus

increased activated T cell number ( J:110686 )

• peripheral T cells in mutants older than 2 months display a preactivated phenotype; lymph node T cells express high levels of CD44 and CD69 compared to wild-type while 2 to 3-fold more T cells express low levels of CD62L

abnormal spleen germinal center morphology ( J:110686 )

• enlarged

abnormal lymphocyte physiology ( J:110686 )

• a normal proliferative response is mounted by lymph node T cells when exposed the superantigen staphylococcal enterotoxin (SEA) or increasing CD3-specific antibodies in vitro but a 3- to 4-fold increase in proliferation is detected in the secondary response after restimulation with antigen

abnormal B cell physiology ( J:110686 )

• 10% of mature B cells show an activated phenotype in the thymus

abnormal T cell physiology ( J:110686 )

• mutants exhibit a gradual increase in peripheral lymphocytes; up to 2 months of age, numbers are similar to wild-type but the numbers increase 2-fold and up to 5- to 6-fold in mice older than 6 months

• after in vivo stimulation with SEA for 8 days, CD4+Vb11+ T cells show normal expansion but distinctly retarded deletion compared to wild-type (incomplete antigen response termination)

endocrine/exocrine glands

enlarged thymus ( J:110686 )

thymus hyperplasia ( J:110686 )

• thymi of mice that are 6 months old show an increased cellularity compared to wild-type

abnormal thymus involution ( J:110686 )

• animals over 6 months of age show abnormal involution; thymi of mutants exhibit large cortical areas similar to young wild-type mice

growth/size/body

enlarged spleen ( J:110686 )

• spleens of mutants are massively enlarged due to extended white pulp areas