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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Ets1tm2Jml
targeted mutation 2, Jeffrey M Leiden
MGI:2182780
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Ets1tm2Jml/Ets1tm2Jml involves: 129X1/SvJ * C57BL/6 MGI:3608775
hm2
Ets1tm2Jml/Ets1tm2Jml involves: 129X1/SvJ * C57BL/6J MGI:7541419
ht3
Ets1tm2Jml/Ets1+ involves: 129X1/SvJ * C57BL/6J MGI:7541420
cn4
Ets1tm2Jml/Ets1tm2Jml
Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+
Pax3tm1(cre)Joe/Pax3+
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCrl MGI:7541421


Genotype
MGI:3608775
hm1
Allelic
Composition
Ets1tm2Jml/Ets1tm2Jml
Genetic
Background
involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ets1tm2Jml mutation (0 available); any Ets1 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• approximately 50% of homozygotes survive until 4 weeks of age

hematopoietic system
N
• normal numbers of splenic IgM+ B220+ B cells
• splenocytes have normal expression of IL2RB, IL2RG, IL15RA and IL18
• increased numbers of splenic and lymph node plasma cells with serum IgM levels elevated by approximately 5 fold
• reduced NK cell numbers evidenced by diminished splenic DX5+ CD3- cells and diminished splenic NK1.1+ CD3- cells
• lymph nodes and bone marrow do not contain detectable DX5+ CD3- cells
• slightly reduced numbers of splenic T cells, particularly CD8+ T cells
• 4 to 8 week old homozygotes have approximately 65% reduction in thymocyte numbers with low levels of CD4 expressed on many CD8+ thymocytes
• poly (I:C) stimulated splenocytes produce reduced amounts of IFNG and IL12
• splenocytes fail to lyse YAC-1 tumor cells, B2m-/- lyphoid blasts, and MHC class I deficient RMA-S tumor cells are not rejected at a normal rate in vivo
• addition of IL12, IL2 and IL15 fails to restore YAC-1 cytolysis
• splenic T cells display a defect in activation following cross-linking of the T cell receptor

immune system
• increased numbers of splenic and lymph node plasma cells with serum IgM levels elevated by approximately 5 fold
• reduced NK cell numbers evidenced by diminished splenic DX5+ CD3- cells and diminished splenic NK1.1+ CD3- cells
• lymph nodes and bone marrow do not contain detectable DX5+ CD3- cells
• slightly reduced numbers of splenic T cells, particularly CD8+ T cells
• 4 to 8 week old homozygotes have approximately 65% reduction in thymocyte numbers with low levels of CD4 expressed on many CD8+ thymocytes
• poly (I:C) stimulated splenocytes produce reduced amounts of IFNG and IL12
• splenocytes fail to lyse YAC-1 tumor cells, B2m-/- lyphoid blasts, and MHC class I deficient RMA-S tumor cells are not rejected at a normal rate in vivo
• addition of IL12, IL2 and IL15 fails to restore YAC-1 cytolysis
• splenic T cells display a defect in activation following cross-linking of the T cell receptor

endocrine/exocrine glands
• 4 to 8 week old homozygotes have approximately 65% reduction in thymocyte numbers with low levels of CD4 expressed on many CD8+ thymocytes

cellular
• splenic T cells display a defect in activation following cross-linking of the T cell receptor




Genotype
MGI:7541419
hm2
Allelic
Composition
Ets1tm2Jml/Ets1tm2Jml
Genetic
Background
involves: 129X1/SvJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ets1tm2Jml mutation (0 available); any Ets1 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• at E14.5, LacZ staining showed a decreased number of cardiac neural crest cells at the aortic valve
• septation of the proximal outflow tract (OFT) is impaired
• at E15.5, three of 4 hearts show DORV phenotypes by MRI analysis
• at E14.5, all (4 of 4) hearts show DORV by serial section analysis through the outflow tracts (OFTs)
• at E15.5, one of 4 hearts exhibit an overriding aorta phenotype by MRI analysis
• at E14.5, three of 4 hearts exhibit a bi-leaflet (bisinuate) aortic valve
• by E18.5, the infundibular sleeve consisting of cardiac myocytes is severely reduced; instead of the tendon of the infundibulum, a fibrous outlet septum produces continuity between leaflets of the aortic and pulmonary valves in a side-by-side orientation

embryo
• at E14.5, LacZ staining showed a decreased number of cardiac neural crest cells at the aortic valve

nervous system
• at E14.5, LacZ staining showed a decreased number of cardiac neural crest cells at the aortic valve




Genotype
MGI:7541420
ht3
Allelic
Composition
Ets1tm2Jml/Ets1+
Genetic
Background
involves: 129X1/SvJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ets1tm2Jml mutation (0 available); any Ets1 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
integument
• mice exhibit a patch of white fur on their abdomen

pigmentation
• mice exhibit a patch of white fur on their abdomen




Genotype
MGI:7541421
cn4
Allelic
Composition
Ets1tm2Jml/Ets1tm2Jml
Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+
Pax3tm1(cre)Joe/Pax3+
Genetic
Background
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCrl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ets1tm2Jml mutation (0 available); any Ets1 mutation (28 available)
Gt(ROSA)26Sortm14(CAG-tdTomato)Hze mutation (5 available); any Gt(ROSA)26Sor mutation (993 available)
Pax3tm1(cre)Joe mutation (1 available); any Pax3 mutation (50 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• at E13.5, alpha-actinin staining showed that cardiac myocytes are separated from the tdTomato positive cNCCs in the OFT cushion
• by E15.5, far fewer cardiomyocytes are in contact with tdTomato positive cNCCs in the OFT cushion, indicating impaired muscularization
• by E15.5, cNCCs persist as a fibrous outlet septum in the setting of DORV

embryo
• 12.7% of explanted cultured cardiac neural crest cells (cNCCs) make cell-cell contacts versus 8.5% of control cNCCs; strikingly, 81% of those contacts fail to separate within 2 h versus only 31.2% in control cNCCs, suggesting increased cell-cell adhesion
• at E9.5, fewer tdTomato-labeled cNCCs are detected in the developing outflow tract (OFT) relative to control embryos, suggesting a delay in cNCC migration
• at E10.5, a nearly complete absence of tdTomato cNCCs is seen in the proximal component of the OFT cushions
• lack of tdTomato cNCCs in the proximal outflow cushion is less severe at E11.5; abundant cNCCs are found in the distal and intermediate outflow cushions, but not in proximal cushions, consistent with delayed migration
• moreover, number of Pax3Cre-tdTomato expressing cells that co-express SOX10 is markedly decreased and the linear migration pattern from the neural tube toward the heart is disrupted; most of tdTomato positive-expressing cells lacking SOX10 expression are located rather peripherally
• N-cadherin staining showed upregulation of N-cadherin expression in migratory NCCs at E8.5 and E9.5
• time-lapse image analysis of explanted cultured cNCCs showed a significant decrease in migration velocity relative to controls

cellular
• 12.7% of explanted cultured cardiac neural crest cells (cNCCs) make cell-cell contacts versus 8.5% of control cNCCs; strikingly, 81% of those contacts fail to separate within 2 h versus only 31.2% in control cNCCs, suggesting increased cell-cell adhesion
• at E9.5, fewer tdTomato-labeled cNCCs are detected in the developing outflow tract (OFT) relative to control embryos, suggesting a delay in cNCC migration
• at E10.5, a nearly complete absence of tdTomato cNCCs is seen in the proximal component of the OFT cushions
• lack of tdTomato cNCCs in the proximal outflow cushion is less severe at E11.5; abundant cNCCs are found in the distal and intermediate outflow cushions, but not in proximal cushions, consistent with delayed migration
• moreover, number of Pax3Cre-tdTomato expressing cells that co-express SOX10 is markedly decreased and the linear migration pattern from the neural tube toward the heart is disrupted; most of tdTomato positive-expressing cells lacking SOX10 expression are located rather peripherally
• N-cadherin staining showed upregulation of N-cadherin expression in migratory NCCs at E8.5 and E9.5
• time-lapse image analysis of explanted cultured cNCCs showed a significant decrease in migration velocity relative to controls





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last database update
11/19/2024
MGI 6.24
The Jackson Laboratory