Phenotypes associated with this allele

• Allele Symbol: Per2^tm1Drw
• Allele Name: targeted mutation 1, David R Weaver
• Allele ID: MGI:2182832
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Per2^tm1Drw/Per2^tm1Drw	B6.129-Per2^tm1Drw/J	MGI:5576760
hm2	Per2^tm1Drw/Per2^tm1Drw	involves: 129	MGI:3852066
cx3	Per1^tm1Drw/Per1^tm1Drw Per2^tm1Drw/Per2^tm1Drw	involves: 129S4/SvJae	MGI:3852065
cx4	Per2^tm1Drw/Per2^tm1Drw Per3^tm1Drw/Per3^tm1Drw	involves: 129/Sv * 129S4/SvJae	MGI:3852067
cx5	Per2^tm1Drw/Per2^tm1Drw Tg(PER2*S662D)382Ljp/0	involves: C57BL/6	MGI:3852101
cx6	Per2^tm1Drw/Per2^tm1Drw Tg(PER2*S662G)867Ljp/0	involves: C57BL/6	MGI:3852104

Genotype
MGI:5576760

hm1

• Allelic Composition: Per2^tm1Drw/Per2^tm1Drw
• Genetic Background: B6.129-Per2^tm1Drw/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

abnormal blood vessel morphology ( J:208598 )

• mice show a 25% reduction in the number of vessels in the sciatic nerve indicating that the network of vasa nervorum is reduced

abnormal retina vasculature morphology ( J:208598 )

• 40% increase in acellular capillaries in the retina indicating retinal vascular damage

hematopoietic system

abnormal bone marrow cell morphology/development ( J:208598 )

• mice exhibit bone marrow neuropathy, with a decrease in tyrosine hydroxylase-positive and neurofilament 200-positive nerve processes at 4 and 12 months of age

increased hematopoietic stem cell number ( J:208598 )

• 4 month old mice show a 2-fold increase in lin- sca1+ c-Kit+ (LSK) cells within the bone marrow, indicating a dysfunction in bone marrow progenitor cell (BMPC) release from bone marrow

abnormal hematopoietic stem cell physiology ( J:208598 )

• BMPCs show decreased colony formation and NO levels

decreased hematopoietic stem cell proliferation ( J:208598 )

• BMPCs show a 3-fold decrease in proliferation

cellular

decreased hematopoietic stem cell proliferation ( J:208598 )

• BMPCs show a 3-fold decrease in proliferation

homeostasis/metabolism

improved glucose tolerance ( J:208598 )

• mice are normoglycemic and show better recovery after glucose challenge than wild-type mice

nervous system

abnormal nervous system morphology ( J:208598 )

• mice exhibit bone marrow neuropathy, with a decrease in tyrosine hydroxylase-positive and neurofilament 200-positive nerve processes at 4 and 12 months of age, respectively

vision/eye

abnormal retina morphology ( J:208598 )

• increase in retinal permeability

• dissociation of tight junctions and adherens junctional integrity in the retina

abnormal retina vasculature morphology ( J:208598 )

• 40% increase in acellular capillaries in the retina indicating retinal vascular damage

Genotype
MGI:3852066

hm2

• Allelic Composition: Per2^tm1Drw/Per2^tm1Drw
• Genetic Background: involves: 129

behavior/neurological

arrhythmic circadian behavior persistence ( J:69626 )

• all mice become arrhythmic when transferred into dark-dark conditions although at different duration and extent of rhythm persistence

abnormal sleep pattern ( J:95746 )

• at the midday 3-hour period, mice wake more than wild-type mice

• at midday, mice exhibit less REM sleep than wild-type mice

• during the 12-hour recovery after 6 hours of prolonged waking, mice exhibit less waking and more short wave sleep compared to similarly treated wild-type mice

• mice are awake more during the mid-third of the light period compared with wild-type mice

homeostasis/metabolism

abnormal circadian temperature homeostasis ( J:95746 )

• the acrophase of core temperature is phase-advanced compared to in wild-type mice

Genotype
MGI:3852065

cx3

• Allelic Composition: Per1^tm1Drw/Per1^tm1Drw; Per2^tm1Drw/Per2^tm1Drw
• Genetic Background: involves: 129S4/SvJae

behavior/neurological

arrhythmic circadian behavior persistence ( J:69626 , J:95746 )

• mice immediately lose their rhythmicity when transferred into dark-dark conditions (J:69626)

• in free-running conditions, mice become arrhythmic immediately under dark-dark conditions unlike similarly treated wild-type mice (J:95746)

abnormal sleep pattern ( J:95746 )

• during the 12-hour light segment, mice wake more and have less slow wave sleep compared to wild-type mice

• during the last two-third of the lights-on period, mice exhibit more wakefulness than wild-type mice

• at midday, mice exhibit less REM sleep than wild-type mice

• in dark dark conditions, mice exhibit arrhythmic pattern of time spent in wakefulness, short wave sleep, and REM sleep unlike similarly treated wild-type mice

• however, the amount of sleep obtained in dark-dark conditions is normal

• during entrained conditions, mice exhibit longer wake bouts during the dark period compared with the light period unlike in wild-type mice

• during entrained conditions, mice exhibit longer short wave sleep and REM sleep bouts during the light segment compared with wild-type mice

• arrhythmic mice exhibit even distribution of wake, short wave sleep, and REM bouts during subjective light and dark periods unlike wild-type mice

homeostasis/metabolism

abnormal circadian temperature homeostasis ( J:95746 )

• the acrophase of core temperature is phase-advanced compared to in wild-type mice

nervous system

abnormal brain wave pattern ( J:95746 )

• recovery after 6 hours of prolonged waking, mice exhibit an increase in delta power lasting 12 hours during the recovery sleep period compared with wild-type mice in which it lasts 3 hours

Genotype
MGI:3852067

cx4

• Allelic Composition: Per2^tm1Drw/Per2^tm1Drw; Per3^tm1Drw/Per3^tm1Drw
• Genetic Background: involves: 129/Sv * 129S4/SvJae

behavior/neurological

arrhythmic circadian behavior persistence ( J:69626 )

• as in Per2^tm1Drw homozygotes

Genotype
MGI:3852101

cx5

• Allelic Composition: Per2^tm1Drw/Per2^tm1Drw; Tg(PER2*S662D)382Ljp/0
• Genetic Background: involves: C57BL/6

behavior/neurological

prolonged circadian behavior period ( J:126404 )

• circadian period is 0.7 hours longer than in wild-type mice

Genotype
MGI:3852104

cx6

• Allelic Composition: Per2^tm1Drw/Per2^tm1Drw; Tg(PER2*S662G)867Ljp/0
• Genetic Background: involves: C57BL/6

behavior/neurological

shortened circadian behavior period ( J:126404 )

• circadian period is shortened 3.3 hours compared to in wild-type mice

• however, mice under LD10.5:10.5 conditions exhibit normal on-off activity patterns