Phenotypes associated with this allele

• Allele Symbol: Adcyap1^tm1Aba
• Allele Name: targeted mutation 1, Akemichi Baba
• Allele ID: MGI:2182878
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Adcyap1^tm1Aba/Adcyap1^tm1Aba	either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * ICR)	MGI:2447608
hm2	Adcyap1^tm1Aba/Adcyap1^tm1Aba	involves: 129P2/OlaHsd * C57BL/6	MGI:5898413
hm3	Adcyap1^tm1Aba/Adcyap1^tm1Aba	involves: 129P2/OlaHsd * C57BL/6 * ICR	MGI:3822734

Genotype
MGI:2447608

hm1

• Allelic Composition: Adcyap1^tm1Aba/Adcyap1^tm1Aba
• Genetic Background: either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * ICR)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, incomplete penetrance ( J:72576 )

• incomplete penetrance

behavior/neurological

increased exploration in new environment ( J:72576 )

• increased activity in open field

decreased anxiety-related response ( J:72576 )

• reduced anxiety in open field emergence, elevated plus maze, and novel-object tests

decreased grooming behavior ( J:72576 )

hyperactivity ( J:72576 )

increased vertical activity ( J:72576 )

• rearing and jumping

reproductive system

reduced fertility ( J:72576 )

• homozygous matings resulted in fewer successful pregnanacies than littermate controls

reduced female fertility ( J:102717 )

♀ • Background Sensitivity: female fertility is more severely reduced on a mixed 129P2/OlaHsd x C57BL/6 genetic background (only 33% vs 85% in wild-type females) than on an outbred genetic background following 4-6 backcrosses onto the ICR strain (50% vs 87% in wild-type females)

Genotype
MGI:5898413

hm2

• Allelic Composition: Adcyap1^tm1Aba/Adcyap1^tm1Aba
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

vision/eye

abnormal cornea morphology ( J:240066 )

• 40% of males over 30 weeks of age exhibit corneas with hypertrophy of the surface and keratinization

• less than 20% of females younger than 10 weeks of age exhibit corneal keratinization and more than 90% of females over 30 weeks of age show keratinization

• however, the infraorbital lacrimal gland, conjunctiva and corneal neural network are normal

abnormal cornea epithelium morphology ( J:240066 )

• corneal epithelial cells are hypertrophied

cornea opacity ( J:240066 )

• cloudiness of the cornea and the ocular surface appears white and sandy

decreased tear production ( J:240066 )

• tear secretion levels and tear volume of males and females are reduced

• mice treated with eye drops containing a peptide of ADYCAP1 P(ACAP38) show increased tear secretion and repeated treatments prevents further corneal keratinization

Genotype
MGI:3822734

hm3

• Allelic Composition: Adcyap1^tm1Aba/Adcyap1^tm1Aba
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * ICR

behavior/neurological

absent maternal crouching ( J:102717 )

♀ • following 4-6 backcrosses onto the ICR strain, female homozygotes with a reduced litter size spend significantly less time crouching over their pups than wild-type females

♀ • however, other maternal behaviors, such as nesting, sniffing, and pup retrieval, are normal

♀ • Background Sensitivity: crouching time is significantly reduced on an ICR-enriched genetic background but remains relatively unaffected on a mixed 129P2/OlaHsd x C57BL/6 genetic background where litter size is normal

abnormal mating frequency ( J:102717 )

• following 4-6 backcrosses onto the ICR strain, female homozygotes display a reduced mating frequency relative to wild-type controls (58% vs 93%, respectively), as indicated by the presence of a vaginal plug

• after mating with vasectomized males, 3 of 9 female homozygotes showed an abnormal pseudopregnancy response, whereas another three did not mate, with a single exception, and the remaining three showed normal responses; in contrast, all wild-type control females mated with an interval of 11-12 days

• however, no obvious defects are observed in the length of estrous cycles

reproductive system

reduced female fertility ( J:102717 )

♀ • Background Sensitivity: following 4-6 backcrosses onto ICR, the % of female homozygotes that deliver pups is increased to 50% relative to only 33% of female homozygotes on a mixed 129P2/OlaHsd x C57BL/6 genetic background

decreased litter size ( J:102717 )

• Background Sensitivity: the average litter size is not significantly different between homozygous mutant females and wild-type control females on a mixed 129P2/OlaHsd x C57BL/6 genetic background (9.00 0.69 vs 8.55 0.84 pups/litter, respectively) but is significantly reduced on an ICR-enriched genetic background (11.50 0.34 vs 13.54 0.54 pups/litter, respectively)

• following 4-6 backcrosses onto the ICR strain, female homozygotes produce a significantly reduced litter size relative to wild-type female controls