Phenotypes associated with this allele

• Allele Symbol: Hrh1^tm1Wtn
• Allele Name: targeted mutation 1, Takeshi Watanabe
• Allele ID: MGI:2182945
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hrh1^tm1Wtn/Hrh1^tm1Wtn	B6.129P2-Hrh1^tm1Wtn	MGI:3761138
hm2	Hrh1^tm1Wtn/Hrh1^tm1Wtn	involves: 129P2/OlaHsd * C57BL/6	MGI:3761125

Genotype
MGI:3761138

hm1

• Allelic Composition: Hrh1^tm1Wtn/Hrh1^tm1Wtn
• Genetic Background: B6.129P2-Hrh1^tm1Wtn

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

increased brown adipose tissue amount ( J:107191 )

• the amount of brown adipose tissue at 48 weeks of age is increased relative to in wild-type mice

• insulin levels during a 4 week feeding schedule are lower than in mice fed ad libitum

• however, the levels of brown adipose tissue at 12 weeks of age is normal

increased white adipose tissue amount ( J:107191 )

• at 28 weeks of age, white adipose tissue is increased by 23%, 48% and 40% in the epididymmal, mesenteric and retroperitoneal fat pads, respectively, compared to wild-type mice

decreased total body fat amount ( J:107191 )

• unlike in wild-type mice, intraperitoneal administration of the histamine agonist methyl(2[2-pyridyl]ethyl)amine dyhydrochloride (1 ug/g for 7 days) results in decreased in adiposity

increased fat cell size ( J:107191 )

• adipocytes in the epididymal white adipose tissue are larger than in wild-type mice at 48 weeks of age

• however, adipocyte size at 12 weeks of age is normal

muscle

increased skeletal muscle triglyceride level ( J:107191 )

• mice exhibit 20% more triglycerides in skeletal muscle compared to wild-type mice

homeostasis/metabolism

decreased circulating insulin level ( J:107191 )

• at 48 weeks of age, serum insulin levels are increased relative to in wild-type mice

• however, at 12 weeks of age serum insulin levels are normal

increased circulating leptin level ( J:107191 )

• at 48 weeks of age, leptin levels are increased by 66% compared to in wild-type mice

• however, at 12 weeks of age leptin levels are normal

increased circulating free fatty acids level ( J:107191 )

• at 48 weeks of age, free fatty acid levels are increased relative to in wild-type mice

• however, at 12 weeks of age free fatty acid levels are normal

increased liver triglyceride level ( J:107191 )

• mice exhibit 45% more triglycerides in the liver compared to wild-type mice

increased skeletal muscle triglyceride level ( J:107191 )

• mice exhibit 20% more triglycerides in skeletal muscle compared to wild-type mice

abnormal response/metabolism to endogenous compounds ( J:107191 )

• following intracerebroventricular administration of 0.5 ug leptin or intraperitoneal injection of 50 ug of leptin, mice exhibit a smaller decrease in food consumption and body mass relative to wild-type

behavior/neurological

abnormal circadian feeding behavior ( J:107191 )

• at 48 weeks, the ratio of food consumed in the light versus dark phase is smaller than in wild-type mice

decreased food intake ( J:107191 )

• unlike in wild-type mice, intraperitoneal administration of the histamine agonist methyl(2[2-pyridyl]ethyl)amine dyhydrochloride (1 ug/g for 7 days) results in decreased food intake

• however, intracerebroventricular administration of methyl(2[2-pyridyl]ethyl)amine dyhydrochloride has no effect on mice

increased food intake ( J:107191 )

• at 48 weeks of age, mice consume slightly more food than wild-type mice

• however, at 12 weeks of age food consumption is normal

growth/size/body

decreased body weight ( J:107191 )

• during 4 week on a feeding schedule, mice fail to gain weight as do wild-type mice despite equivalent food consumption

• unlike in wild-type mice, intraperitoneal administration of the histamine agonist methyl(2[2-pyridyl]ethyl)amine dyhydrochloride (1 ug/g for 7 days) results in decreased body mass

• however, intracerebroventricular administration of methyl(2[2-pyridyl]ethyl)amine dyhydrochloride has no effect on mice

increased body weight ( J:107191 )

• after 28 weeks of age mice exhibit increased body mass relative to wild-type mice

immune system

increased IgG level ( J:110367 )

• following ovalbumin challenge, IgG1, IgG2a and IgG2b levels are increased relative to similarly treated wild-type mice

increased IgM level ( J:110367 )

• following ovalbumin challenge, IgM levels are increased relative to similarly treated wild-type mice

decreased interferon-gamma secretion ( J:110367 )

• antigen stimulation of splenocytes results in the production of less interferon-gamma compared to in similarly treated wild-type mice

decreased interleukin-13 secretion ( J:110367 )

• antigen stimulation of splenocytes results in the production of less IL-13 compared to in similarly treated wild-type mice

decreased interleukin-5 secretion ( J:110367 )

• antigen stimulation of splenocytes results in the production of less IL-5 compared to in similarly treated wild-type mice

decreased inflammatory response ( J:110367 )

• following challenge with ovalbumin, mice fail to develop lung inflammation as do wild-type mice with fewer eosinophils and lymphocytes detected in the bronchoalveolar lavage

• however, mice exhibit normal B cell immune responses and inhalation of IL-4 or IL-13 induces lung inflammation

liver/biliary system

increased liver triglyceride level ( J:107191 )

• mice exhibit 45% more triglycerides in the liver compared to wild-type mice

hematopoietic system

increased IgG level ( J:110367 )

• following ovalbumin challenge, IgG1, IgG2a and IgG2b levels are increased relative to similarly treated wild-type mice

increased IgM level ( J:110367 )

• following ovalbumin challenge, IgM levels are increased relative to similarly treated wild-type mice

Genotype
MGI:3761125

hm2

• Allelic Composition: Hrh1^tm1Wtn/Hrh1^tm1Wtn
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

decreased B cell proliferation ( J:111704 )

• B cell proliferation in response to anti-IgM is reduced 3- to 4-fold compared to in wild-type mice

• however, B cell proliferation in response to IL-4 alone or in combination with CD40LG or LPS is normal

decreased T cell proliferation ( J:111704 )

• T cell proliferation in response to anti-CD3-epsilon is reduced 5- to 8-fold compared to in wild-type mice

• T cell proliferation in response to ovalbmin is reduced 4- to 6-fold compared to in wild-type mice

• however, T cell proliferation in response to PHA is normal

decreased T cell number ( J:111704 )

• the number of CD5+ B220+ T cells is slightly decreased (21.0+/-8.6% compared to 39.5+/-9.1% in wild-type mice)

decreased IgG3 level ( J:111704 )

• IgG3 levels are slightly lower than in wild-type mice

• following challenge with ovalbumin, anti-ovalbumin IgG3 antibodies are lower than in similarly treated wild-type mice

decreased IgM level ( J:111704 )

• following stimulation with TNP-Ficoll, anti-TNP IgM antibodies are 9-fold lower than in similarly treated wild-type mice

• following challenge with ovalbumin, anti-ovalbumin IgM antibodies are lower than in similarly treated wild-type mice

decreased interferon-gamma secretion ( J:111704 )

• following in vitro stimulation with ovalbumin or anti-CD3 epsilon antibodies, cells produce less interferon-gamma than wild-type mice

decreased interleukin-2 secretion ( J:111704 )

• following in vitro stimulation with ovalbumin or anti-CD3 epsilon antibodies, cells produce less IL-2

increased interleukin-4 secretion ( J:111704 )

• following in vitro stimulation with ovalbumin or anti-CD3 epsilon antibodies, cells produce more IL-4 than wild-type mice

behavior/neurological

abnormal behavior ( J:36556 )

• during passive avoidance testing, mice display more frequent urination and defecation and decreased movement compared to wild-type mice

• however, passive avoidance behavior is normal

decreased exploration in new environment ( J:36556 )

• despite normal motor coordination, mice exhibit decreased ambulation and time of rearing for the first 30 minutes when placed in a new environment

abnormal locomotor circadian rhythm ( J:36556 )

• mice exhibit increased locomotor activity during early portions of the light phase and decreased ambulation during the dark phase of a 12 hours light 12 hour dark cycle relative to similarly treated wild-type mice

• the ratio of ambulation during the light phase to total ambulation during 24 hours is increased compared to in wild-type mice

• however, total ambulation during a 24 hours period is normal

decreased vertical activity ( J:36556 )

• mice rear less when placed in a new environment compared to wild-type mice

hematopoietic system

decreased B cell proliferation ( J:111704 )

• B cell proliferation in response to anti-IgM is reduced 3- to 4-fold compared to in wild-type mice

• however, B cell proliferation in response to IL-4 alone or in combination with CD40LG or LPS is normal

decreased T cell proliferation ( J:111704 )

• T cell proliferation in response to anti-CD3-epsilon is reduced 5- to 8-fold compared to in wild-type mice

• T cell proliferation in response to ovalbmin is reduced 4- to 6-fold compared to in wild-type mice

• however, T cell proliferation in response to PHA is normal

decreased T cell number ( J:111704 )

• the number of CD5+ B220+ T cells is slightly decreased (21.0+/-8.6% compared to 39.5+/-9.1% in wild-type mice)

decreased IgG3 level ( J:111704 )

• IgG3 levels are slightly lower than in wild-type mice

• following challenge with ovalbumin, anti-ovalbumin IgG3 antibodies are lower than in similarly treated wild-type mice

decreased IgM level ( J:111704 )

• following stimulation with TNP-Ficoll, anti-TNP IgM antibodies are 9-fold lower than in similarly treated wild-type mice

• following challenge with ovalbumin, anti-ovalbumin IgM antibodies are lower than in similarly treated wild-type mice

cellular

decreased B cell proliferation ( J:111704 )

• B cell proliferation in response to anti-IgM is reduced 3- to 4-fold compared to in wild-type mice

• however, B cell proliferation in response to IL-4 alone or in combination with CD40LG or LPS is normal

decreased T cell proliferation ( J:111704 )

• T cell proliferation in response to anti-CD3-epsilon is reduced 5- to 8-fold compared to in wild-type mice

• T cell proliferation in response to ovalbmin is reduced 4- to 6-fold compared to in wild-type mice

• however, T cell proliferation in response to PHA is normal