Phenotypes associated with this allele

• Allele Symbol: Olig2^{tm1(cre/Esr1*)Htak}
• Allele Name: targeted mutation 1, Hirohide Takebayashi
• Allele ID: MGI:2183410
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Olig2^tm1(cre/Esr1*)Htak/Olig2^tm1(cre/Esr1*)Htak	involves: BALB/c * C57BL/6 * C57BL/6NCrj * CBA	MGI:3810326
hm2	Olig2^tm1(cre/Esr1*)Htak/Olig2^tm1(cre/Esr1*)Htak	involves: C57BL/6NCrlj * CBA/JNCrlj	MGI:5426962

Genotype
MGI:3810326

hm1

• Allelic Composition: Olig2^{tm1(cre/Esr1*)Htak}/Olig2^{tm1(cre/Esr1*)Htak}
• Genetic Background: involves: BALB/c * C57BL/6 * C57BL/6NCrj * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:77821 )

• mice do not feed and die on the day of birth

nervous system

decreased oligodendrocyte progenitor number ( J:77821 )

• at E18.5, oligodendrocyte progenitors are absent from the spinal cord with neuroepithelial cells of the ventral spinal cord becoming astrocytes instead

abnormal neuronal migration ( J:77821 )

• unlike in wild type mice, the few motor neurons that form in the pMN domain migrate into the lateral margin of the cord similar to p2 domain neurons

abnormal spinal cord morphology ( J:77821 )

• at E10.5, few motor neurons are found in the spinal cord

• at E18.5, oligodendrocyte progenitors are absent from the spinal cord

decreased motor neuron number ( J:77821 )

• at E10.5, few motor neurons are found in the spinal cord

• at E18.5, the ventral horn of the spinal cord lacks motorneurons

abnormal spinal cord ventral horn morphology ( J:77821 )

• at E18.5, the ventral horn of the spinal cord lacks motorneurons

• motor neurons and oligodendrocyte progenitors fail to develop in the ventral spinal cord

behavior/neurological

aphagia ( J:77821 )

• mice do not feed

abnormal reflex ( J:77821 )

• at E18.5, reflex movement when mice are touched is uncoordinated compared to in wild-type mice

abnormal posture ( J:77821 )

• mice retain their in utero posture after birth

muscle

hypotonia ( J:77821 )

• mice lack tonicity in extension muscles

cellular

decreased oligodendrocyte progenitor number ( J:77821 )

• at E18.5, oligodendrocyte progenitors are absent from the spinal cord with neuroepithelial cells of the ventral spinal cord becoming astrocytes instead

abnormal neuronal migration ( J:77821 )

• unlike in wild type mice, the few motor neurons that form in the pMN domain migrate into the lateral margin of the cord similar to p2 domain neurons

Genotype
MGI:5426962

hm2

• Allelic Composition: Olig2^{tm1(cre/Esr1*)Htak}/Olig2^{tm1(cre/Esr1*)Htak}
• Genetic Background: involves: C57BL/6NCrlj * CBA/JNCrlj

nervous system

nervous system phenotype ( J:182750 )

N • number of nociceptive (TrkA-positive) neurons is not different from controls

decreased motor neuron number ( J:182750 )

• at E10.5, no motor neurons are detected in the spinal cords of homozygotes

abnormal sensory neuron morphology ( J:182750 )

• number of TrkB-positive mechanoreceptive-neurons is significantly reduced in the dorsal root ganglia (DRG) at E13.5, but difference from wild-type is not significant at E18.5

abnormal sensory neuron innervation pattern ( J:182750 )

• abnormal axonal projection of peripheral branches of sensory neurons is observed at E10.5 and 12.5; abnormal sensory axonal projection and fasciculation is detected by neurofilament staining at E15.5

abnormal proprioceptive neuron morphology ( J:182750 )

• number of TrkC- or Runx3-positive proprioceptive neurons is decreased significantly in the DRGs at E13.5 compared to heterozygous controls; Runx3-positive proprioceptive neurons are decreased in number in DRGs at E18.5

abnormal dorsal root ganglion morphology ( J:182750 )

• at E10.5, an increased number of cleaved Casp3-positive apoptotic cells is observed in the DRGs relative to heterozygous controls; at E13.5, this difference is not significant

• number of proliferating Ki-67-positive cells is similar in homozygotes and controls at E10.5 and E13.5

cellular

abnormal cell differentiation ( J:203419 )

• cultured neural precursor cells have an impaired ability to produce oligodendrocytes