Phenotypes associated with this allele

• Allele Symbol: Clec10a^tm1Hed
• Allele Name: targeted mutation 1, Stephen M Hedrick
• Allele ID: MGI:2183546
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Clec10a^tm1Hed/Clec10a^tm1Hed	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:2672840
cx2	Clec10a^tm1Hed/Clec10a^tm1Hed St3gal1^tm1Jxm/St3gal1^tm1Jxm	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:3850151
cx3	Clec10a^tm1Hed/Clec10a^+ St3gal1^tm1Jxm/St3gal1^tm1Jxm	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:3850152

Genotype
MGI:2672840

hm1

• Allelic Composition: Clec10a^tm1Hed/Clec10a^tm1Hed
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:283174 )

N • normal skin barrier function after exposure to house dust mites (HDMs)

hematopoietic system

increased erythrocyte cell number ( J:78035 )

• homozygotes display a slight but significant (3.5%) increase in red blood cell count relative to wild-type mice

• however, no changes are detected in red cell distribution width, and mutant erythrocyte life spans remain relatively unaffected as determined after biotinylation in vivo

increased hematocrit ( J:78035 )

• homozygotes show a slight but significant (6.0 %) increase in hematocrit levels relative to wild-type mice

increased hemoglobin content ( J:78035 )

• homozygotes show a slight but significant (3.8%) increase in hemoglobin content relative to wild-type mice

decreased mean corpuscular hemoglobin ( J:78035 )

• homozygotes show a slight but significant (1.8%) decrease in mean corpuscular hemoglobin concentration relative to wild-type mice

increased mean corpuscular volume ( J:78035 )

• homozygotes show only a slight (2.4%) increase in mean corpuscular volume relative to wild-type mice

increased neutrophil cell number ( J:283174 )

• increased neutrophil infiltration in skin 6 days after exposure to house dust mites (HDMs)

thrombocytosis ( J:78035 )

• homozygotes show a 13.2% increase in platelet cell number relative to wild-type mice

integument

integument phenotype ( J:283174 )

N • normal skin barrier function after exposure to house dust mites (HDMs)

dermatitis ( J:283174 )

• hypersensitivity to house dust mite (HDM)-induced skin inflammation (erythema, dryness, increased epidermal thickness)

immune system

immune system phenotype ( J:78035 , J:283174 )

N • homozygotes exhibit a normal thymus as well as normal macrophage function and T- and B-cell development relative to control mice (J:78035)

N • in addition, homozygotes show a normal cytotoxic T-cell response to major histocompatibility-disparate P815 tumor cells, with no differences in cytolytic activity, CD8 T cell expansion, and proportion of apoptotic (annexin V⁺) CD8 T cells relative to wild-type control mice (J:78035)

N • normal serum IgE and IgG2c levels after exposure to house dust mites (HDMs) (J:283174)

increased neutrophil cell number ( J:283174 )

• increased neutrophil infiltration in skin 6 days after exposure to house dust mites (HDMs)

dermatitis ( J:283174 )

• hypersensitivity to house dust mite (HDM)-induced skin inflammation (erythema, dryness, increased epidermal thickness)

Genotype
MGI:3850151

cx2

• Allelic Composition: Clec10a^tm1Hed/Clec10a^tm1Hed; St3gal1^tm1Jxm/St3gal1^tm1Jxm
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

hematopoietic system

decreased CD8-positive, alpha-beta T cell number ( J:78035 )

• although at 3 and 5 weeks of age, female (but not male) double homozygotes show a significant increase of CD8-positive T-cell numbers in the periphery relative to mice that are heterozygous for Mgl1^tm1Hed and homozygous for St3gal1^tm1Jxm, double homozygotes still exhibit a profound CD8 T-cell deficiency relative to wild-type mice

immune system

decreased CD8-positive, alpha-beta T cell number ( J:78035 )

• although at 3 and 5 weeks of age, female (but not male) double homozygotes show a significant increase of CD8-positive T-cell numbers in the periphery relative to mice that are heterozygous for Mgl1^tm1Hed and homozygous for St3gal1^tm1Jxm, double homozygotes still exhibit a profound CD8 T-cell deficiency relative to wild-type mice

Genotype
MGI:3850152

cx3

• Allelic Composition: Clec10a^tm1Hed/Clec10a⁺; St3gal1^tm1Jxm/St3gal1^tm1Jxm
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

hematopoietic system

decreased CD8-positive, alpha-beta T cell number ( J:78035 )

• at 3 to 6 weeks of age, mutant mice exhibit a variable reduction in the percentage of CD8 T cells in peripheral blood relative to heterozygous St3gal1^tm1Jxm control mice (1-5% vs 11%, respectively)

immune system

decreased CD8-positive, alpha-beta T cell number ( J:78035 )

• at 3 to 6 weeks of age, mutant mice exhibit a variable reduction in the percentage of CD8 T cells in peripheral blood relative to heterozygous St3gal1^tm1Jxm control mice (1-5% vs 11%, respectively)