Phenotypes associated with this allele

• Allele Symbol: Sftpa1^tm1Kor
• Allele Name: targeted mutation 1, Thomas R Korfhagen
• Allele ID: MGI:2183737
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sftpa1^tm1Kor/Sftpa1^tm1Kor	B6.129P2-Sftpa1^tm1Kor	MGI:3794770
hm2	Sftpa1^tm1Kor/Sftpa1^tm1Kor	involves: 129P2/OlaHsd	MGI:3794739
hm3	Sftpa1^tm1Kor/Sftpa1^tm1Kor	involves: 129P2/OlaHsd * Black Swiss * C3H/HeN	MGI:3794763
hm4	Sftpa1^tm1Kor/Sftpa1^tm1Kor	involves: 129P2/OlaHsd * NIH Black Swiss	MGI:3794735

Genotype
MGI:3794770

hm1

• Allelic Composition: Sftpa1^tm1Kor/Sftpa1^tm1Kor
• Genetic Background: B6.129P2-Sftpa1^tm1Kor

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

respiratory system

abnormal surfactant physiology ( J:130520 )

• following infection with mycoplasma, the numbers of large surfactant aggregates is decreased and higher protein to lipid ratios are present in the bronchoalveolar lavage fluid compared to similarly infected wild-type mice

homeostasis/metabolism

abnormal phospholipid level ( J:130520 )

• mice exhibit a decrease in phospholipid content of the VLA (very large aggregates) fraction of the bronchalveolar lavage (BAL) fluid and an increase in the phospolipid levels of the ILA (intermediate large aggregates) fraction compared to in wild-type mice

• however, the total phospolipid content of the BAL is normal

Genotype
MGI:3794739

hm2

• Allelic Composition: Sftpa1^tm1Kor/Sftpa1^tm1Kor
• Genetic Background: involves: 129P2/OlaHsd

respiratory system

lung inflammation ( J:107841 )

• LPS-induced injury increases the number of alveolar resident cells compared to in similarly treated wild-type mice

abnormal surfactant physiology ( J:107841 )

• LPS-induced injury decreases surfactant clearance 40% compared to in similarly treated wild-type mice

homeostasis/metabolism

abnormal phospholipid level ( J:107841 )

• LPS-induced injury increases the level of phospholipids in lavage fluid 1.6-fold compared to in similarly treated wild-type mice

immune system

immune system phenotype ( J:130958 )

N • mice exhibit a normal inflammatory response following infection with Cryptococcus neoformans

lung inflammation ( J:107841 )

• LPS-induced injury increases the number of alveolar resident cells compared to in similarly treated wild-type mice

Genotype
MGI:3794763

hm3

• Allelic Composition: Sftpa1^tm1Kor/Sftpa1^tm1Kor
• Genetic Background: involves: 129P2/OlaHsd * Black Swiss * C3H/HeN

mortality/aging

postnatal lethality, complete penetrance ( J:130297 )

• all mice born in a corn dust environment (resulting in exposure to various bacteria) die within 48 hours

immune system

increased susceptibility to bacterial infection ( J:130297 )

• mice born in a corn dust environment exhibit gastrointestinal abnormalities and high bacteria count in peritoneal fluid unlike wild-type mice

• all mice born in a corn dust environment (resulting in exposure to various bacteria) die within 48 hours

Genotype
MGI:3794735

hm4

• Allelic Composition: Sftpa1^tm1Kor/Sftpa1^tm1Kor
• Genetic Background: involves: 129P2/OlaHsd * NIH Black Swiss

immune system

immune system phenotype ( J:35250 )

N • mice do not exhibit any altered lung inflammation

lung inflammation ( J:110382 )

• lung infiltrates are observed 6 hours after exposure to group B streptococcus (GBS) compared to in similarly treated wild-type mice that show infiltrate after 24 hours

• at 48 hours post-infection with GBS, inflammation persists and is more severe than in similarly treated wild-type mice

increased susceptibility to bacterial infection ( J:110382 , J:120552 )

• lung infiltrates are observed 6 hours after exposure to group B streptococcus (GBS) compared to in similarly treated wild-type mice that show infiltrate after 24 hours (J:110382)

• at 48 hours post-infection with GBS, inflammation persists and is more severe than in similarly treated wild-type mice mice exhibit reduced bacterial clearance compared to wild-type mice (J:110382)

• mice exhibit increased systemic infection with GBS compared to wild-type mice with increased splenic burden (J:110382)

• GBS associated with alveolar macrophages is decreased compared to in wild-type mice (J:110382)

• mice exhibit increased susceptibility to infection with Pneumocystis carinii compared to wild-type mice (J:120552)

respiratory system

respiratory system phenotype ( J:35250 )

N • mice do not exhibit any altered lung structure, alveolar phospholipids pool size or composition, or lung inflammation

lung inflammation ( J:110382 )

• lung infiltrates are observed 6 hours after exposure to group B streptococcus (GBS) compared to in similarly treated wild-type mice that show infiltrate after 24 hours

• at 48 hours post-infection with GBS, inflammation persists and is more severe than in similarly treated wild-type mice

abnormal surfactant physiology ( J:111449 )

• surfactant exhibits decreased minimum surface tension and increased sensitivity to plasma inactivation compared to wild-type surfactant

• mice exhibit fewer large aggregates of surfactant compared to in wild-type mice

• the number of large surfactant aggregates following exposure to NMU (N-nitroso-N-methylurea) is decreased compared to in similarly treated wild-type mice