Phenotypes associated with this allele

• Allele Symbol: Vegfa^tm2.1Nagy
• Allele Name: targeted mutation 2.1, Andras Nagy
• Allele ID: MGI:2383985
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Vegfa^tm2.1Nagy/Vegfa^tm2.1Nagy	involves: 129S1/Sv * 129X1/SvJ * CD-1	MGI:2446608
ht2	Vegfa^tm2.1Nagy/Vegfa^+	involves: 129S1/Sv * 129X1/SvJ	MGI:4422208
cn3	Vegfa^tm2Gne/Vegfa^tm2.1Nagy Tg(Nes-cre)1Kln/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL	MGI:4422207

Genotype
MGI:2446608

hm1

• Allelic Composition: Vegfa^tm2.1Nagy/Vegfa^tm2.1Nagy
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * CD-1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between somite formation and embryo turning, complete penetrance ( J:75937 )

• die between E8.5 and E9

embryo

abnormal vitelline vasculature morphology ( J:75937 )

• disorganized yolk sac vasculature with large lacunae

• reduction in the number of blood islands devoid of primitive erythrocytes

cardiovascular system

abnormal dorsal aorta morphology ( J:75937 )

• reduced lumina of the dorsal aortae

decreased vascular endothelial cell number ( J:75937 )

• lack mature endothelial cells as indicated by the severely reduced expression of Flt1 (Vegf-R1)

abnormal vitelline vasculature morphology ( J:75937 )

• disorganized yolk sac vasculature with large lacunae

• reduction in the number of blood islands devoid of primitive erythrocytes

delayed heart development ( J:75937 )

• development of the heart is delayed

• however, the endocardium is formed

hematopoietic system

pancytopenia ( J:75937 )

• reduction in the number of blood cells in the embryo proper, particularly in the sinus venosus

• lack mature hematopoietic cells as indicated by the severely reduced expression of Klf1 (Eklf)

Genotype
MGI:4422208

ht2

• Allelic Composition: Vegfa^tm2.1Nagy/Vegfa⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

cardiovascular system

abnormal brain vasculature morphology ( J:86207 )

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the forebrain and cortex while the distance between branch points is increased compared with wild-type mice

abnormal vascular branching morphogenesis ( J:86207 )

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the forebrain and cortex while the distance between branch points is increased compared with wild-type mice

nervous system

abnormal brain vasculature morphology ( J:86207 )

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the forebrain and cortex while the distance between branch points is increased compared with wild-type mice

Genotype
MGI:4422207

cn3

• Allelic Composition: Vegfa^tm2Gne/Vegfa^tm2.1Nagy; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL

mortality/aging

neonatal lethality, complete penetrance ( J:86207 )

• mice die of dehydration within 24 hours of birth

nervous system

abnormal brain vasculature morphology ( J:86207 )

• at P1, the cerebral cortex lacks pial vasculature unlike in wild-type mice

• mice exhibit deficient vascular development in the superficial levels of the cortex near the marginal zone and cortical plate, and in the deep ventricular zone compared with wild-type mice

• mice exhibit defects in blood vessel density compared with wild-type mice

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the cortex and forebrain while the distance between branch points is increased compared with wild-type mice

hydrocephaly ( J:86207 )

enlarged lateral ventricles ( J:86207 )

• in neonates

abnormal choroid plexus morphology ( J:86207 )

abnormal cerebral cortex morphology ( J:86207 )

• at P1, the cerebral cortex is decreased in size compared to in wild-type mice

• at P1, the cerebral cortex lacks pial vasculature and exhibits cortical degeneration unlike in wild-type mice

• mice exhibit altered cortical neuronal organization compared with wild-type mice

neuron degeneration ( J:86207 )

• at E13.5, mice exhibit neuron degeneration in the forebrain unlike wild-type mice

• mice exhibit neuron degeneration mainly in the subventricular zone unlike wild-type mice

• at E15.5, mice exhibit overt and anterior specific cortical neuronal degeneration unlike wild-type mice

abnormal neuron physiology ( J:86207 )

• by E15.5, mice exhibit an increase in neuron apoptosis in the cortex compared with wild-type mice

abnormal neuron proliferation ( J:86207 )

• mice exhibit a 25% reduction in proliferating neurons compared with wild-type mice

• at E13.5, mice exhibit a 60% reduction in the number of periventricular neurons in the forebrain compared with wild-type mice

cardiovascular system

abnormal brain vasculature morphology ( J:86207 )

• at P1, the cerebral cortex lacks pial vasculature unlike in wild-type mice

• mice exhibit deficient vascular development in the superficial levels of the cortex near the marginal zone and cortical plate, and in the deep ventricular zone compared with wild-type mice

• mice exhibit defects in blood vessel density compared with wild-type mice

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the cortex and forebrain while the distance between branch points is increased compared with wild-type mice

abnormal retina vasculature morphology ( J:86207 )

• at P1, sprouting angiogenesis into the inner plexiform layer is absent unlike in wild-type mice

abnormal vascular branching morphogenesis ( J:86207 )

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the cortex and forebrain while the distance between branch points is increased compared with wild-type mice

decreased angiogenesis ( J:86207 )

• at E15.5, decreased vessel density and lack of sprouting is pronounced compared to in wild-type mice

behavior/neurological

absent gastric milk in neonates ( J:86207 )

abnormal motor capabilities/coordination/movement ( J:86207 )

• neonates exhibit spastic uncontrolled movements unlike wild-type mice

• neonates fail to remain upright when placed in a prone position unlike wild-type mice

homeostasis/metabolism

dehydration ( J:86207 )

• mice die of dehydration within 24 hours of birth

hypoxia ( J:86207 )

• between E11.5 and E13.5 in the forebrain and throughout the CNS by E15.5

craniofacial

abnormal craniofacial morphology ( J:86207 )

• as early as E17.5, mice exhibit abnormal cranial morphology compared with wild-type mice

• neonates exhibit altered cranial morphology compared to in wild-type mice

vision/eye

abnormal retina vasculature morphology ( J:86207 )

• at P1, sprouting angiogenesis into the inner plexiform layer is absent unlike in wild-type mice

cellular

abnormal neuron proliferation ( J:86207 )

• mice exhibit a 25% reduction in proliferating neurons compared with wild-type mice

• at E13.5, mice exhibit a 60% reduction in the number of periventricular neurons in the forebrain compared with wild-type mice