Analysis Tools
endocrine/exocrine glands
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• at 8 weeks of age, mutant ovaries show no evidence of antral follicles or corpora lutea
• stromal cells are relatively hypertrophied, with the interstitial cells containing numerous small lipid droplets
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• no corpora lutea are detected at 8 weeks of age
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• although numerous small follicles are observed, no large antral follicles can be found at 8 weeks of age
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• ovarian folliculogenesis is arrested at the preantral stage
• treatment with PMSG for 4 days has no effect on mutant ovarian histology and fails to stimulate the uterus
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small ovary
(
J:61909
)
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• at 8 weeks of age, the ovarian weight of female homozygotes is significantly lower than that of wild-type and heterozygous littermates (1.25 0.15 vs. 3.62 0.52 and 2.64 0.21 mg, respectively)
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• at 8 weeks of age, seminiferous tubule diameter of male homozygotes is reduced relative to that of wild-type males
• however, all stages of spermatogenesis are observed and Leydig cell morphology appears unaffected
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• in contrast, seminal vesicle weight remains unaffected at this age
• at 8 weeks of age, testis weight in male homozygotes is 50% of that in wild-type and heterozygous littermates
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• at 8 weeks of age, serum levels of both inhibin A and inhibin B are below the detection level of the assays in all four mice and in the ovaries of 3 of the 4 mice analyzed
• in contrast, testicular and serum inhibin A and B concentrations are not significantly different from those in wild-type littermates
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homeostasis/metabolism
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• at 8 weeks of age, serum LH levels of both male and female homozygotes are significantly elevated relative to wild-type levels
• in female homozygotes, mean serum LH levels are 28-fold higher than in control females
• pituitary content of LH is not significantly altered in male or female homozygotes relative to control mice
• no differences are noted between the pituitary weight of female or male homozygotes and wild-type controls at 8 weeks of age
• ovariectomy of female homozygotes had no significant effect on serum levels or pituitary content of LH
• treatment of both intact and ovariectomized female homozygotes with 17beta-estradiol for 1 week, significantly reduces serum LH to wild-type levels
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• at 8 weeks of age, pituitary levels of FSH are elevated in female, but not male, homozygotes relative to wild-type levels
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• at 8 weeks of age, serum levels of FSH of both male and female homozygotes are significantly elevated relative to wild-type levels
• ovariectomy of female homozygotes had no significant effect on serum levels or pituitary content of FSH
• although treatment of both intact and ovariectomized female homozygotes with 17beta-estradiol for 1 week reduced serum FSH levels by 50%, these remained significantly higher than levels in wild-type females
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reproductive system
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• at 8 weeks of age, mutant ovaries show no evidence of antral follicles or corpora lutea
• stromal cells are relatively hypertrophied, with the interstitial cells containing numerous small lipid droplets
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• no corpora lutea are detected at 8 weeks of age
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• although numerous small follicles are observed, no large antral follicles can be found at 8 weeks of age
|
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• ovarian folliculogenesis is arrested at the preantral stage
• treatment with PMSG for 4 days has no effect on mutant ovarian histology and fails to stimulate the uterus
|
small ovary
(
J:61909
)
|
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• at 8 weeks of age, the ovarian weight of female homozygotes is significantly lower than that of wild-type and heterozygous littermates (1.25 0.15 vs. 3.62 0.52 and 2.64 0.21 mg, respectively)
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• at 8 weeks of age, seminiferous tubule diameter of male homozygotes is reduced relative to that of wild-type males
• however, all stages of spermatogenesis are observed and Leydig cell morphology appears unaffected
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• at 8 weeks of age, testis weight in male homozygotes is 50% of that in wild-type and heterozygous littermates
• in contrast, seminal vesicle weight remains unaffected at this age
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• at 8 weeks of age, serum levels of both inhibin A and inhibin B are below the detection level of the assays in all four mice and in the ovaries of 3 of the 4 mice analyzed
• in contrast, testicular and serum inhibin A and B concentrations are not significantly different from those in wild-type littermates
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• at 8 weeks of age, the uterine weight of female homozygotes is remarkably lower than that of wild-type and heterozygous littermates (5.9 1.9 mg vs. 83.3 21.4 mg and 66.3 8.7 mg, respectively)
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• at 8 weeks, mutant uteri are extremely thin relative to wild-type
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• at 8 weeks of age, the vagina is imperforate
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• female homozygotes show no evidence of oestrus cyclicity
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• when female homoygyzotes are caged with males of proven fertility over a period of 6 months, no pups are born
• in contrast, male homozygotes are fertile and produce an average litter of normal size
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