All Phenotypes Tg(TLK2mHEL)2Ccg MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0	involves: 129S/SvEv * C57BL/6	MGI:3799693
cx2	Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0	involves: C57BL/6 * C57BL/10 * C57BR/cd	MGI:3799692
cx3	Tg(ML5sHEL)5Ccg/0 Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0	involves: C57BL/6JSfd * C57BL/10 * C57BR/cd	MGI:3799695

Allelic Composition	Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0
Genetic Background	involves: 129S/SvEv * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2^tm1Fwa mutation (45 available); any Rag2 mutation (119 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)
Tg(TLK2mHEL)2Ccg mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

thyroid gland inflammation ( J:78309 )

• 54% of mice develop thyroiditis compared to 100% of mice carrying both transgenes on a Rag2⁺ background

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• the number of CD4 T cells in the spleen and lymph nodes is severely reduced

• there are 25-fold fewer peripheral T cells present than in Rag2 null homozygotes carrying just the TCR transgene

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

thyroid gland inflammation ( J:78309 )

• 54% of mice develop thyroiditis compared to 100% of mice carrying both transgenes on a Rag2⁺ background

hematopoietic system

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• the number of CD4 T cells in the spleen and lymph nodes is severely reduced

• there are 25-fold fewer peripheral T cells present than in Rag2 null homozygotes carrying just the TCR transgene

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

thyroid gland inflammation ( J:78309 )

• thyroids are extensively infiltrated by T cells bearing transgenic TCR, B cells and some macrophages

• however, no symptoms of hypothyroidism occur

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost two-thirds

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

thyroid gland inflammation ( J:78309 )

• thyroids are extensively infiltrated by T cells bearing transgenic TCR, B cells and some macrophages

• however, no symptoms of hypothyroidism occur

hematopoietic system

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost two-thirds

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

Allelic Composition	Tg(ML5sHEL)5Ccg/0 Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0
Genetic Background	involves: C57BL/6JSfd * C57BL/10 * C57BR/cd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(ML5sHEL)5Ccg mutation (2 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)
Tg(TLK2mHEL)2Ccg mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased double-positive T cell number ( J:78309 )

• there is a 32-fold reduction in the number of double positive T cells in the thymus

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost three-quarters

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

immune system

thyroid gland inflammation ( J:78309 )

• spontaneous thyroiditis does not occur in these mice as it does in mice doubly transgenic for Tg(ML5sHEL)5Ccg and Tg(TcrHEL3A9)1Mmd transgene

decreased double-positive T cell number ( J:78309 )

• there is a 32-fold reduction in the number of double positive T cells in the thymus

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost three-quarters

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

thyroid gland inflammation ( J:78309 )

• spontaneous thyroiditis does not occur in these mice as it does in mice doubly transgenic for Tg(ML5sHEL)5Ccg and Tg(TcrHEL3A9)1Mmd transgene

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 12/10/2024 MGI 6.24