All Phenotypes Tg(TLK2mHEL)2Ccg MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0	involves: 129S/SvEv * C57BL/6	MGI:3799693
cx2	Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0	involves: C57BL/6 * C57BL/10 * C57BR/cd	MGI:3799692
cx3	Tg(ML5sHEL)5Ccg/0 Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0	involves: C57BL/6JSfd * C57BL/10 * C57BR/cd	MGI:3799695

Allelic Composition	Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0
Genetic Background	involves: 129S/SvEv * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2^tm1Fwa mutation (45 available); any Rag2 mutation (119 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)
Tg(TLK2mHEL)2Ccg mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

thyroid gland inflammation ( J:78309 )

• 54% of mice develop thyroiditis compared to 100% of mice carrying both transgenes on a Rag2⁺ background

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• the number of CD4 T cells in the spleen and lymph nodes is severely reduced

• there are 25-fold fewer peripheral T cells present than in Rag2 null homozygotes carrying just the TCR transgene

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

thyroid gland inflammation ( J:78309 )

• 54% of mice develop thyroiditis compared to 100% of mice carrying both transgenes on a Rag2⁺ background

hematopoietic system

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• the number of CD4 T cells in the spleen and lymph nodes is severely reduced

• there are 25-fold fewer peripheral T cells present than in Rag2 null homozygotes carrying just the TCR transgene

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

thyroid gland inflammation ( J:78309 )

• thyroids are extensively infiltrated by T cells bearing transgenic TCR, B cells and some macrophages

• however, no symptoms of hypothyroidism occur

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost two-thirds

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

thyroid gland inflammation ( J:78309 )

• thyroids are extensively infiltrated by T cells bearing transgenic TCR, B cells and some macrophages

• however, no symptoms of hypothyroidism occur

hematopoietic system

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost two-thirds

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

Allelic Composition	Tg(ML5sHEL)5Ccg/0 Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0
Genetic Background	involves: C57BL/6JSfd * C57BL/10 * C57BR/cd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(ML5sHEL)5Ccg mutation (2 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)
Tg(TLK2mHEL)2Ccg mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased double-positive T cell number ( J:78309 )

• there is a 32-fold reduction in the number of double positive T cells in the thymus

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost three-quarters

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

immune system

thyroid gland inflammation ( J:78309 )

• spontaneous thyroiditis does not occur in these mice as it does in mice doubly transgenic for Tg(ML5sHEL)5Ccg and Tg(TcrHEL3A9)1Mmd transgene

decreased double-positive T cell number ( J:78309 )

• there is a 32-fold reduction in the number of double positive T cells in the thymus

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost three-quarters

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

thyroid gland inflammation ( J:78309 )

• spontaneous thyroiditis does not occur in these mice as it does in mice doubly transgenic for Tg(ML5sHEL)5Ccg and Tg(TcrHEL3A9)1Mmd transgene

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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