Phenotypes associated with this allele

• Allele Symbol: Pik3cd^tm1.1Bvan
• Allele Name: targeted mutation 1.1, Bart Vanhaesebroeck
• Allele ID: MGI:2385596
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pik3cd^tm1.1Bvan/Pik3cd^tm1.1Bvan	B6.129P2-Pik3cd^tm1.1Bvan	MGI:3796441
hm2	Pik3cd^tm1.1Bvan/Pik3cd^tm1.1Bvan	involves: 129P2/OlaHsd	MGI:3796334
hm3	Pik3cd^tm1.1Bvan/Pik3cd^tm1.1Bvan	involves: 129P2/OlaHsd * BALB/c	MGI:2449978

Genotype
MGI:3796441

hm1

• Allelic Composition: Pik3cd^tm1.1Bvan/Pik3cd^tm1.1Bvan
• Genetic Background: B6.129P2-Pik3cd^tm1.1Bvan

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal platelet aggregation ( J:114310 )

• platelets exhibit slower shape change and decreased rate and amplitude of aggregation compared to wild-type platelets in response to submaximal concentrations of adenosine diphosphate or phorbol ester, or intermediate and high levels of collagen-related peptide

• platelet spreading on fibrinogen is impaired compared to wild-type platelets

• however, adhesion and aggregate formation at an intermediate rate of shear is normal

homeostasis/metabolism

abnormal platelet aggregation ( J:114310 )

• platelets exhibit slower shape change and decreased rate and amplitude of aggregation compared to wild-type platelets in response to submaximal concentrations of adenosine diphosphate or phorbol ester, or intermediate and high levels of collagen-related peptide

• platelet spreading on fibrinogen is impaired compared to wild-type platelets

• however, adhesion and aggregate formation at an intermediate rate of shear is normal

Genotype
MGI:3796334

hm2

• Allelic Composition: Pik3cd^tm1.1Bvan/Pik3cd^tm1.1Bvan
• Genetic Background: involves: 129P2/OlaHsd

cardiovascular system

cardiovascular system phenotype ( J:136236 )

N • vascular development is normal

Genotype
MGI:2449978

hm3

• Allelic Composition: Pik3cd^tm1.1Bvan/Pik3cd^tm1.1Bvan
• Genetic Background: involves: 129P2/OlaHsd * BALB/c

digestive/alimentary system

abnormal intestinal epithelium morphology ( J:78412 )

• mixed leukocyte infiltrates and regenerative changes in the glandular epithelium

abnormal intestinal mucosa morphology ( J:78412 )

• mucosal hyperplasia

crypts of Lieberkuhn abscesses ( J:78412 )

intestinal inflammation ( J:78412 )

• animals develop mild inflammatory bowel disease

endocrine/exocrine glands

crypts of Lieberkuhn abscesses ( J:78412 )

hematopoietic system

abnormal B cell differentiation ( J:78412 )

• CD23-CD21+IgM+ marginal zone B cells nearly undetectable

increased neutrophil cell number ( J:78412 )

• peripheral blood neutrophils increased two-fold

decreased B-1 B cell number ( J:78412 )

• CD5+IgM+ peritoneal B-1 cells nearly undetectable

spleen hypoplasia ( J:78412 )

• 50% reduction in spleen cell count compared to wild-type

absent spleen germinal center ( J:78412 )

decreased immunoglobulin level ( J:78412 )

• decreased serum level of immunoglobulin

decreased IgA level ( J:78412 )

• IgM and IgA most commonly reduced

decreased IgM level ( J:78412 )

• IgM and IgA most commonly reduced

immune system

intestinal inflammation ( J:78412 )

• animals develop mild inflammatory bowel disease

abnormal B cell differentiation ( J:78412 )

• CD23-CD21+IgM+ marginal zone B cells nearly undetectable

increased neutrophil cell number ( J:78412 )

• peripheral blood neutrophils increased two-fold

decreased B-1 B cell number ( J:78412 )

• CD5+IgM+ peritoneal B-1 cells nearly undetectable

spleen hypoplasia ( J:78412 )

• 50% reduction in spleen cell count compared to wild-type

absent spleen germinal center ( J:78412 )

decreased immunoglobulin level ( J:78412 )

• decreased serum level of immunoglobulin

decreased IgA level ( J:78412 )

• IgM and IgA most commonly reduced

decreased IgM level ( J:78412 )

• IgM and IgA most commonly reduced

abnormal Peyer's patch germinal center morphology ( J:78412 )

• absence of germinal centers