All Phenotypes Tg(TcrHEL3A9)1Mmd MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Tg(TcrHEL3A9)1Mmd
transgene insertion 1, Mark M Davis
MGI:2385683

Summary

10 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Ncaph2^nes/Ncaph2^nes Tg(TcrHEL3A9)1Mmd/0	C57BL/6-Ncaph2^nes Tg(TcrHEL3A9)1Mmd	MGI:3718590
cx2	Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0	involves: 129S/SvEv * C57BL/6	MGI:3799693
cx3	Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcrHEL3A9)1Mmd/0	involves: 129S/SvEv * C57BL/6	MGI:3799694
cx4	Tg(ML5sHEL)5Ccg/0 Tg(TcrHEL3A9)1Mmd/0	involves: C57BL/6 * C57BL/10 * C57BR/cd	MGI:3799690
cx5	Tg(KLK4mHEL)6Ccg/0 Tg(TcrHEL3A9)1Mmd/0	involves: C57BL/6 * C57BL/10 * C57BR/cd	MGI:3799688
cx6	Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0	involves: C57BL/6 * C57BL/10 * C57BR/cd	MGI:3799692
cx7	Tg(ML5sHEL)5Ccg/0 Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0	involves: C57BL/6JSfd * C57BL/10 * C57BR/cd	MGI:3799695
cx8	Tg(ILK3mHEL)3Ccg/0 Tg(TcrHEL3A9)1Mmd/0	involves: C57BL/6JSfd * C57BL/10Sg * C57BR/cd	MGI:3799685
tg9	Tg(TcrHEL3A9)1Mmd/0	involves: C57BL/6	MGI:3799372
tg10	Tg(TcrHEL3A9)1Mmd/0	involves: C57BL/6 * C57BL/10 * C57BR/cd	MGI:3799684

Allelic Composition	Ncaph2^nes/Ncaph2^nes Tg(TcrHEL3A9)1Mmd/0
Genetic Background	C57BL/6-Ncaph2^nes Tg(TcrHEL3A9)1Mmd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ncaph2^nes mutation (3 available); any Ncaph2 mutation (38 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal thymus cell ratio ( J:123300 )

• decrease in the proportion of double positive cells and increase in the proportion of DN4 cells

abnormal double-negative T cell morphology ( J:123300 )

• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression in the thymus

decreased double-positive T cell number ( J:123300 )

• decrease in the proportion of double positive cells in the thymus similar to homozygous mice not expressing the prearranged T cell receptor transgene

hematopoietic system

abnormal thymus cell ratio ( J:123300 )

• decrease in the proportion of double positive cells and increase in the proportion of DN4 cells

abnormal double-negative T cell morphology ( J:123300 )

• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression in the thymus

decreased double-positive T cell number ( J:123300 )

• decrease in the proportion of double positive cells in the thymus similar to homozygous mice not expressing the prearranged T cell receptor transgene

endocrine/exocrine glands

abnormal thymus cell ratio ( J:123300 )

• decrease in the proportion of double positive cells and increase in the proportion of DN4 cells

Allelic Composition	Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0
Genetic Background	involves: 129S/SvEv * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2^tm1Fwa mutation (45 available); any Rag2 mutation (119 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)
Tg(TLK2mHEL)2Ccg mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

thyroid gland inflammation ( J:78309 )

• 54% of mice develop thyroiditis compared to 100% of mice carrying both transgenes on a Rag2⁺ background

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• the number of CD4 T cells in the spleen and lymph nodes is severely reduced

• there are 25-fold fewer peripheral T cells present than in Rag2 null homozygotes carrying just the TCR transgene

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

thyroid gland inflammation ( J:78309 )

• 54% of mice develop thyroiditis compared to 100% of mice carrying both transgenes on a Rag2⁺ background

hematopoietic system

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• the number of CD4 T cells in the spleen and lymph nodes is severely reduced

• there are 25-fold fewer peripheral T cells present than in Rag2 null homozygotes carrying just the TCR transgene

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

Allelic Composition	Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcrHEL3A9)1Mmd/0
Genetic Background	involves: 129S/SvEv * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2^tm1Fwa mutation (45 available); any Rag2 mutation (119 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal thymus cell ratio ( J:78309 )

• there is a skewing towards the CD-4 T cell subset in mice on a homozygote H2^k/k background

• the ratio of T cell subsets in the thymus is skewed toward CD8 T cells on a heterozygote H2^b/k background

abnormal double-positive T cell morphology ( J:78309 )

• 55.9% of the double-positive thymocytes express the transgenic TCR

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• 15% of total lymphocytes found in lymph nodes are CD4 T cells bearing the transgenic TCR

• CD4 T cells that express the transgenic TCR-beta chain also express low or high amounts of the TCR-alpha chain

• the CD4 T cells expressing the low amounts of transgenic TCR-alpha may also express endogenous TCR-alpha chains

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• the number of CD4 T cells in the spleen and lymph nodes is severely reduced

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR proliferate in response to hen egg lysozyme

• proliferation occurs whether CD4 T cells are low or high expressers of the transgenic TCR-alpha chain

hematopoietic system

abnormal thymus cell ratio ( J:78309 )

• there is a skewing towards the CD-4 T cell subset in mice on a homozygote H2^k/k background

• the ratio of T cell subsets in the thymus is skewed toward CD8 T cells on a heterozygote H2^b/k background

abnormal double-positive T cell morphology ( J:78309 )

• 55.9% of the double-positive thymocytes express the transgenic TCR

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• 15% of total lymphocytes found in lymph nodes are CD4 T cells bearing the transgenic TCR

• CD4 T cells that express the transgenic TCR-beta chain also express low or high amounts of the TCR-alpha chain

• the CD4 T cells expressing the low amounts of transgenic TCR-alpha may also express endogenous TCR-alpha chains

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• the number of CD4 T cells in the spleen and lymph nodes is severely reduced

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR proliferate in response to hen egg lysozyme

• proliferation occurs whether CD4 T cells are low or high expressers of the transgenic TCR-alpha chain

endocrine/exocrine glands

abnormal thymus cell ratio ( J:78309 )

• there is a skewing towards the CD-4 T cell subset in mice on a homozygote H2^k/k background

• the ratio of T cell subsets in the thymus is skewed toward CD8 T cells on a heterozygote H2^b/k background

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR proliferate in response to hen egg lysozyme

• proliferation occurs whether CD4 T cells are low or high expressers of the transgenic TCR-alpha chain

Allelic Composition	Tg(ML5sHEL)5Ccg/0 Tg(TcrHEL3A9)1Mmd/0
Genetic Background	involves: C57BL/6 * C57BL/10 * C57BR/cd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(ML5sHEL)5Ccg mutation (2 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased double-positive T cell number ( J:78309 )

• there is a 32-fold reduction in the number of double positive T cells in the thymus

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost three-quarters

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

hematopoietic system

decreased double-positive T cell number ( J:78309 )

• there is a 32-fold reduction in the number of double positive T cells in the thymus

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost three-quarters

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

Allelic Composition	Tg(KLK4mHEL)6Ccg/0 Tg(TcrHEL3A9)1Mmd/0
Genetic Background	involves: C57BL/6 * C57BL/10 * C57BR/cd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(KLK4mHEL)6Ccg mutation (2 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased double-positive T cell number ( J:78309 )

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• there is a reduced number of CD4-positive thymocytes bearing the transgenic TCR

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 10-fold compared to mice transgenic just for the TCR

hematopoietic system

decreased double-positive T cell number ( J:78309 )

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• there is a reduced number of CD4-positive thymocytes bearing the transgenic TCR

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 10-fold compared to mice transgenic just for the TCR

Allelic Composition	Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0
Genetic Background	involves: C57BL/6 * C57BL/10 * C57BR/cd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcrHEL3A9)1Mmd mutation (6 available)
Tg(TLK2mHEL)2Ccg mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

thyroid gland inflammation ( J:78309 )

• thyroids are extensively infiltrated by T cells bearing transgenic TCR, B cells and some macrophages

• however, no symptoms of hypothyroidism occur

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost two-thirds

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

thyroid gland inflammation ( J:78309 )

• thyroids are extensively infiltrated by T cells bearing transgenic TCR, B cells and some macrophages

• however, no symptoms of hypothyroidism occur

hematopoietic system

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost two-thirds

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

Allelic Composition	Tg(ML5sHEL)5Ccg/0 Tg(TcrHEL3A9)1Mmd/0 Tg(TLK2mHEL)2Ccg/0
Genetic Background	involves: C57BL/6JSfd * C57BL/10 * C57BR/cd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(ML5sHEL)5Ccg mutation (2 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)
Tg(TLK2mHEL)2Ccg mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased double-positive T cell number ( J:78309 )

• there is a 32-fold reduction in the number of double positive T cells in the thymus

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost three-quarters

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

immune system

thyroid gland inflammation ( J:78309 )

• spontaneous thyroiditis does not occur in these mice as it does in mice doubly transgenic for Tg(ML5sHEL)5Ccg and Tg(TcrHEL3A9)1Mmd transgene

decreased double-positive T cell number ( J:78309 )

• there is a 32-fold reduction in the number of double positive T cells in the thymus

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by almost three-quarters

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

thyroid gland inflammation ( J:78309 )

• spontaneous thyroiditis does not occur in these mice as it does in mice doubly transgenic for Tg(ML5sHEL)5Ccg and Tg(TcrHEL3A9)1Mmd transgene

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells do not proliferate to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

Allelic Composition	Tg(ILK3mHEL)3Ccg/0 Tg(TcrHEL3A9)1Mmd/0
Genetic Background	involves: C57BL/6JSfd * C57BL/10Sg * C57BR/cd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(ILK3mHEL)3Ccg mutation (6 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

insulitis ( J:78309 )

• 90% of islets are extensively infiltrated by T cells bearing transgenic TCRs, B cells and some macrophages

• however, there are no symptoms of diabetes

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• there is a reduced number of CD4-positive thymocytes

• CD4 T cell numbers in the lymph nodes are reduced by about half

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR have a somewhat reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

hematopoietic system

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• there is a reduced number of CD4-positive thymocytes

• CD4 T cell numbers in the lymph nodes are reduced by about half

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR have a somewhat reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

insulitis ( J:78309 )

• 90% of islets are extensively infiltrated by T cells bearing transgenic TCRs, B cells and some macrophages

• however, there are no symptoms of diabetes

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR have a somewhat reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

Allelic Composition	Tg(TcrHEL3A9)1Mmd/0
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcrHEL3A9)1Mmd mutation (6 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD4-positive, alpha beta T cell morphology ( J:73608 )

• vast majority of CD4 T cells express a T cell receptor that is specific for hen egg lysozyme (HEL) antigen

abnormal T cell activation ( J:73608 )

• CD4 T cells become activated when cultured with HEL protein and B cells from Tg(IghelMD4)4Ccg transgenic mice

• 12 hours of culture leads to upregulation of CD69 on the cell surface

• CD4 T cells enlarge into blasts and express high levels of CD44 after 48 hours in culture

• supernatant from these cultures supports a IL-2/IL-4 dependent cell line

• the use of HEL peptide leads to similar but somewhat reduced levels of activation

increased T cell proliferation ( J:73608 )

abnormal interleukin secretion ( J:73608 )

• CD4 T cells become activated and secrete cytokines when cultured with HEL protein and B cells from Tg(IghelMD4)4Ccg transgenic mice

• supernatant from these cultures supports a IL-2/IL-4 dependent cell line

hematopoietic system

abnormal CD4-positive, alpha beta T cell morphology ( J:73608 )

• vast majority of CD4 T cells express a T cell receptor that is specific for hen egg lysozyme (HEL) antigen

abnormal T cell activation ( J:73608 )

• CD4 T cells become activated when cultured with HEL protein and B cells from Tg(IghelMD4)4Ccg transgenic mice

• 12 hours of culture leads to upregulation of CD69 on the cell surface

• CD4 T cells enlarge into blasts and express high levels of CD44 after 48 hours in culture

• supernatant from these cultures supports a IL-2/IL-4 dependent cell line

• the use of HEL peptide leads to similar but somewhat reduced levels of activation

increased T cell proliferation ( J:73608 )

cellular

increased T cell proliferation ( J:73608 )

Allelic Composition	Tg(TcrHEL3A9)1Mmd/0
Genetic Background	involves: C57BL/6 * C57BL/10 * C57BR/cd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcrHEL3A9)1Mmd mutation (6 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal thymus cell ratio ( J:78309 )

• there is a skewing towards the CD-4 T cell subset in mice on a homozygote H2^k/k background

• the ratio of T cell subsets in the thymus is skewed toward CD8 T cells on a heterozygote H2^b/k background

abnormal double-positive T cell morphology ( J:78309 )

• 55.9% of the double-positive thymocytes express the transgenic TCR

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• 15% of total lymphocytes found in lymph nodes are CD4 T cells bearing the transgenic TCR

• CD4 T cells that express the transgenic TCR-beta chain also express low or high amounts of the TCR-alpha chain

• the CD4 T cells expressing the low amounts of transgenic TCR-alpha may also express endogenous TCR-alpha chains

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by about a third

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR proliferate in response to hen egg lysozyme

• proliferation occurs whether CD4 T cells are low or high expressers of the transgenic TCR-alpha chain

hematopoietic system

abnormal thymus cell ratio ( J:78309 )

• there is a skewing towards the CD-4 T cell subset in mice on a homozygote H2^k/k background

• the ratio of T cell subsets in the thymus is skewed toward CD8 T cells on a heterozygote H2^b/k background

abnormal double-positive T cell morphology ( J:78309 )

• 55.9% of the double-positive thymocytes express the transgenic TCR

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• 15% of total lymphocytes found in lymph nodes are CD4 T cells bearing the transgenic TCR

• CD4 T cells that express the transgenic TCR-beta chain also express low or high amounts of the TCR-alpha chain

• the CD4 T cells expressing the low amounts of transgenic TCR-alpha may also express endogenous TCR-alpha chains

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• CD 4 T cell numbers in the lymph nodes are reduced by about a third

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR proliferate in response to hen egg lysozyme

• proliferation occurs whether CD4 T cells are low or high expressers of the transgenic TCR-alpha chain

endocrine/exocrine glands

abnormal thymus cell ratio ( J:78309 )

• there is a skewing towards the CD-4 T cell subset in mice on a homozygote H2^k/k background

• the ratio of T cell subsets in the thymus is skewed toward CD8 T cells on a heterozygote H2^b/k background

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR proliferate in response to hen egg lysozyme

• proliferation occurs whether CD4 T cells are low or high expressers of the transgenic TCR-alpha chain

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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