Phenotypes associated with this allele

• Allele Symbol: Prkcd^tm1Qxu
• Allele Name: targeted mutation 1, Qingbo Xu
• Allele ID: MGI:2385756
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prkcd^tm1Qxu/Prkcd^tm1Qxu	B6.129P2-Prkcd^tm1Qxu	MGI:5086284
hm2	Prkcd^tm1Qxu/Prkcd^tm1Qxu	involves: 129P2/OlaHsd	MGI:3841772
hm3	Prkcd^tm1Qxu/Prkcd^tm1Qxu	involves: 129P2/OlaHsd * C57BL/6	MGI:3841843
cx4	Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3841844
cx5	Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3841845

Genotype
MGI:5086284

hm1

• Allelic Composition: Prkcd^tm1Qxu/Prkcd^tm1Qxu
• Genetic Background: B6.129P2-Prkcd^tm1Qxu

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Prkcd^tm1Qxu/Prkcd^tm1Qxu mice are protected from age-related hepatosteatosis

mortality/aging

premature death ( J:173902 )

homeostasis/metabolism

improved glucose tolerance ( J:173902 )

increased insulin sensitivity ( J:173902 )

• whole-body and in the liver

decreased liver triglyceride level ( J:173902 )

• mutants are protected from age-related hepatosteatosis, showing lower triglyceride levels in the liver than wild-type mice

growth/size/body

decreased body weight ( J:173902 )

• slightly at 20 weeks when mice are fed standard chow

liver/biliary system

decreased liver triglyceride level ( J:173902 )

• mutants are protected from age-related hepatosteatosis, showing lower triglyceride levels in the liver than wild-type mice

Genotype
MGI:3841772

hm2

• Allelic Composition: Prkcd^tm1Qxu/Prkcd^tm1Qxu
• Genetic Background: involves: 129P2/OlaHsd

immune system

abnormal response to transplant ( J:78680 )

• 8 weeks following vein isografts, intimal lesions are increased compared to in similarly treated wild-type mice

• 8 weeks following vein isografts, 4 of 12 mice exhibit complete occlusion unlike in similarly treated wild-type mice

• veins transplanted into Prkcd^tm1Qxu homozygotes or wild-type mice induce greater intimal lesions compared to when wild-type veins are used

• following vein isografts, the total numbers of smooth muscle cells are higher than in similarly treated wild-type mice

• following vein isografts, the number of apoptotic/necrotic cells is decreased compared to in wild-type mice

cardiovascular system

arteriosclerosis ( J:78680 )

• following vein isografts

vascular smooth muscle hyperplasia ( J:78680 )

• following vein isografts, the total numbers of smooth muscle cells are higher than in similarly treated wild-type mice

abnormal cardiovascular system physiology ( J:78680 )

• smooth muscle cells exhibit decreased cellular sensitivity towards hydrogen peroxide compared to wild-type cells

• following UV and TNF-alpha treatment, smooth muscle cells exhibit decreased apoptosis/necrosis compared to wild-type cells

• following UV and TNF-alpha treatment, smooth muscle cells exhibit reduced reactive oxygen species production compared to similarly treated wild-type cells

muscle

vascular smooth muscle hyperplasia ( J:78680 )

• following vein isografts, the total numbers of smooth muscle cells are higher than in similarly treated wild-type mice

Genotype
MGI:3841843

hm3

• Allelic Composition: Prkcd^tm1Qxu/Prkcd^tm1Qxu
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

premature death ( J:76134 )

• at 9 to 12 months

immune system

immune system phenotype ( J:76134 )

N • despite immune system defects, B cell activation occurs normally

increased B cell apoptosis ( J:76134 )

• B cells are more prone to spontaneous apoptosis and exhibit increased apoptosis in response to anti-Fas or anti-IgM stimulation compared to similarly treated wild-type cells

enlarged spleen ( J:76134 )

increased B cell number ( J:76134 )

• 2-fold in the spleen and 7-fold in the lymph nodes

increased IgA level ( J:76134 )

• mild

increased IgG1 level ( J:76134 )

• mild

increased IgM level ( J:76134 )

• mild

enlarged lymph nodes ( J:76134 )

increased anti-nuclear antigen antibody level ( J:76134 )

• levels of anti-DNA IgG1 are increased compared to in wild-type mice

• at 7 to 9 months, anti-nuclear antibodies are increased compared to in wild-type mice

hematopoietic system

increased B cell apoptosis ( J:76134 )

• B cells are more prone to spontaneous apoptosis and exhibit increased apoptosis in response to anti-Fas or anti-IgM stimulation compared to similarly treated wild-type cells

enlarged spleen ( J:76134 )

increased erythrocyte cell number ( J:76134 )

• Ter-119+ erythroid cells are increased 2-fold in the spleen compared to in wild-type mice

increased B cell number ( J:76134 )

• 2-fold in the spleen and 7-fold in the lymph nodes

increased IgA level ( J:76134 )

• mild

increased IgG1 level ( J:76134 )

• mild

increased IgM level ( J:76134 )

• mild

cellular

increased B cell apoptosis ( J:76134 )

• B cells are more prone to spontaneous apoptosis and exhibit increased apoptosis in response to anti-Fas or anti-IgM stimulation compared to similarly treated wild-type cells

growth/size/body

enlarged spleen ( J:76134 )

Genotype
MGI:3841844

cx4

• Allelic Composition: Prkcd^tm1Qxu/Prkcd^tm1Qxu; Tg(IghelMD4)4Ccg/0; Tg(KLK4mHEL)6Ccg/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

immune system phenotype ( J:76134 )

N • mice exhibit the same number of B cells as in Tg(IghelMD4)4Ccg mice indicating normal clonal deletion

Genotype
MGI:3841845

cx5

• Allelic Composition: Prkcd^tm1Qxu/Prkcd^tm1Qxu; Tg(IghelMD4)4Ccg/0; Tg(ML5sHEL)5Ccg/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

immune system phenotype ( J:76134 )

N • B cell activation in vitro and in vivo are normal

decreased B cell number ( J:76134 )

• peripheral B cells are reduced compared to in Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg mice

abnormal spleen primary B follicle morphology ( J:76134 )

• the number of B follicles is increased compared to in Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

increased spleen germinal center number ( J:76134 )

abnormal B cell physiology ( J:76134 )

• surface levels of IgMa are decreased 2-fold compared to in Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg mice

abnormal B cell proliferation ( J:76134 )

• B cells proliferate vigorously in response to HEL unlike Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

abnormal self tolerance ( J:76134 )

• B cells switch to an autoreactive phenotype and fail to induce tolerance

• B cells proliferate vigorously in response to HEL unlike Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

autoimmune response ( J:76134 )

• mice exhibit an 80-fold increase in HEL-specific antibodies compared to in Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

hematopoietic system

decreased B cell number ( J:76134 )

• peripheral B cells are reduced compared to in Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg mice

abnormal spleen primary B follicle morphology ( J:76134 )

• the number of B follicles is increased compared to in Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

increased spleen germinal center number ( J:76134 )

abnormal B cell physiology ( J:76134 )

• surface levels of IgMa are decreased 2-fold compared to in Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg mice

abnormal B cell proliferation ( J:76134 )

• B cells proliferate vigorously in response to HEL unlike Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

cellular

abnormal B cell proliferation ( J:76134 )

• B cells proliferate vigorously in response to HEL unlike Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice