Phenotypes associated with this allele

• Allele Symbol: Apoe^{tm1(APOE_i4)Sfu}
• Allele Name: targeted mutation 1, Shinobu C Fujita
• Allele ID: MGI:2385931
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Apoe^tm1(APOE_i4)Sfu/Apoe^tm1(APOE_i4)Sfu	involves: 129P2/OlaHsd	MGI:3616189
hm2	Apoe^tm1(APOE_i4)Sfu/Apoe^tm1(APOE_i4)Sfu	involves: 129P2/OlaHsd * C57BL/6N	MGI:2450105
cx3	Apoe^tm1(APOE_i4)Sfu/Apoe^tm1(APOE_i4)Sfu Tg(APPSWE)2576Kha/0	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6N * SJL	MGI:6450215

Genotype
MGI:3616189

hm1

• Allelic Composition: Apoe^{tm1(APOE_i4)Sfu}/Apoe^{tm1(APOE_i4)Sfu}
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

abnormal circulating cholesterol level ( J:104859 )

• serum cholesterol level is increased relative to knock-ins expressing apoE2 or apoE3

abnormal response to CNS ischemic injury ( J:104859 )

• 24 hours after ischemic injury, mice have significantly greater neurologic scores than human APOE2- or APOE3-expressing mice, suggesting poorer outcome

• total infarct area is significantly larger than in knock-ins expressing human APOE2 or APOE3

nervous system

abnormal response to CNS ischemic injury ( J:104859 )

• 24 hours after ischemic injury, mice have significantly greater neurologic scores than human APOE2- or APOE3-expressing mice, suggesting poorer outcome

• total infarct area is significantly larger than in knock-ins expressing human APOE2 or APOE3

cellular

abnormal plasma membrane morphology ( J:103515 )

• abnormal transbilayer distribution of synaptic plasma membrane cholesterol, with a 2-fold increase in exofacial leaflet cholesterol and a reduction in cytofacial leaflet cholesterol, however total amounts of synaptic plasma membrane cholesterol levels were not different from wild-type

Genotype
MGI:2450105

hm2

• Allelic Composition: Apoe^{tm1(APOE_i4)Sfu}/Apoe^{tm1(APOE_i4)Sfu}
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6N

homeostasis/metabolism

increased circulating cholesterol level ( J:60590 )

• serum total cholesterol level is 2.5-fold greater compared to wild-type

increased circulating LDL cholesterol level ( J:60590 )

• 60% of cholesterol is composed of LDL and VLDL

increased circulating VLDL cholesterol level ( J:60590 )

• 60% of cholesterol is composed of LDL and VLDL

decreased brain cholesterol level ( J:60590 )

• 30% reduction in total cholesterol in the brain

abnormal phospholipid level ( J:60590 )

• exhibit a reduction in phospholipid content in the brain

nervous system

abnormal brain morphology ( J:60590 )

• exhibit a reduction in phospholipid content and a 30% reduction in total cholesterol in the brain, although brain size is normal

decreased brain cholesterol level ( J:60590 )

• 30% reduction in total cholesterol in the brain

Genotype
MGI:6450215

cx3

• Allelic Composition: Apoe^{tm1(APOE_i4)Sfu}/Apoe^{tm1(APOE_i4)Sfu}; Tg(APPSWE)2576Kha/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * C57BL/6N * SJL

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:121533 )

♂N • amyloid beta deposits are scarcely seen at 15-16 months of age compared to the many in single Tg(APPSWE)2576Kha/0 mice