Phenotypes associated with this allele

• Allele Symbol: Nr2f2^tm1Tsa
• Allele Name: targeted mutation 1, Sophia Y Tsai
• Allele ID: MGI:2385933
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nr2f2^tm1Tsa/Nr2f2^tm1Tsa	either: 129S7/SvEvBrd or C57BL/6 or (involves: 129S7/SvEvBrd * C57BL/6 * ICR)	MGI:3042231
ht2	Nr2f2^tm1Tsa/Nr2f2^+	either: 129S7/SvEvBrd or C57BL/6 or (involves: 129S7/SvEvBrd * C57BL/6 * ICR)	MGI:3042232
ht3	Nr2f2^tm1Tsa/Nr2f2^+	involves: 129S7/SvEvBrd * C57BL/6	MGI:3717608

Genotype
MGI:3042231

hm1

• Allelic Composition: Nr2f2^tm1Tsa/Nr2f2^tm1Tsa
• Genetic Background: either: 129S7/SvEvBrd or C57BL/6 or (involves: 129S7/SvEvBrd * C57BL/6 * ICR)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:54469 )

• homozygous null embryos died around E10 probably as a result of hemorrhage and edema in the heart and brain

cardiovascular system

decreased angiogenesis ( J:54469 )

• at E9, immunological and molecular analyses of the vascular system revealed a reduction in the extent and complexity of microvasculature in the head and spine regions, suggesting impaired angiogenesis and vascular remodeling

• angiogenic defects were consistent with a loss of function in the mesenchymal compartments of the head, spine, and heart

abnormal vein morphology ( J:54469 )

• by E9-E9.5, both anterior and posterior cardinal veins appeared either malformed or had collapsed

abnormal anterior cardinal vein morphology ( J:54469 )

• by E9-E9.5, anterior cardinal vein appeared either malformed or collapsed

abnormal posterior cardinal vein morphology ( J:54469 )

• by E9-E9.5, posterior cardinal vein appeared either malformed or collapsed

absent trabeculae carneae ( J:54469 )

• at E9.5, some mutant embryos displayed a reduced trabeculation

abnormal heart tube morphology ( J:54469 )

• at E9.5, wild-type hearts displayed an enlarged common atrial chamber, whereas mutant hearts showed only a primitive atrial tube situated on the left

• the primitive atrial tube was connected to a small and primitive sinus venosus situated over the midline; as a result, the mutant pericardial-peritoneal cavity was empty

hemorrhage ( J:54469 )

• at E9.5, homozygous null mice displayed hemorrhage in the frontonasal process and edematous cysts on the brain

• at E10-E10.5, mutant embryos displayed blood-filled brain vesicles and hemorrhagic hearts

embryo

embryo phenotype ( J:120852 )

N • placentas of homozygous mutant embryos developed normally

incomplete somite formation ( J:54469 )

• at E10-E10.5, partially resorbed embryos contained less than 29 somites versus 30-35 somites typically observed in wild-type embryos

muscle

absent trabeculae carneae ( J:54469 )

• at E9.5, some mutant embryos displayed a reduced trabeculation

Genotype
MGI:3042232

ht2

• Allelic Composition: Nr2f2^tm1Tsa/Nr2f2⁺
• Genetic Background: either: 129S7/SvEvBrd or C57BL/6 or (involves: 129S7/SvEvBrd * C57BL/6 * ICR)

mortality/aging

postnatal lethality, incomplete penetrance ( J:54469 )

• approximately two-thirds of heterozygous null mice died before weaning at 21 days postpartum

• poor postnatal viability was confirmed in multiple genetic backgrounds

cardiovascular system

hemorrhage ( J:54469 )

• at E9.5, approximately 5% of heterozygotes exhibited a similar hemorrhagic phenotype as homozygotes

growth/size/body

decreased body size ( J:54469 )

• heterozygous null mice were one-third smaller than wild-type mice by 5 days of birth

Genotype
MGI:3717608

ht3

• Allelic Composition: Nr2f2^tm1Tsa/Nr2f2⁺
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

reproductive system

abnormal ovary morphology ( J:100400 )

♀ • ovaries are less dilated than in wild-type mice

♀ • vascularization of ovaries is reduced

abnormal uterine horn morphology ( J:100400 )

♀ • following treatment with a decidual stimulus, wet weight of the treated horn was less than that of the untreated one

delayed vaginal opening ( J:100400 )

• at 30.6 days compared to 27.9 days in wild-type mice

prolonged diestrus ( J:100400 )

♀

absent estrus ( J:100400 )

♀ • no obvious estrus phase is observed

prolonged metestrus ( J:100400 )

♀

absent estrous cycle ( J:100400 )

♀ • mice lack an obvious estrus phase with prolonged disestrus and metestrus

reduced female fertility ( J:100400 )

♀

decreased litter size ( J:100400 )

• second and third litter sizes are decreased

growth/size/body

postnatal growth retardation ( J:100400 )

homeostasis/metabolism

decreased circulating progesterone level ( J:100400 )

endocrine/exocrine glands

abnormal ovary morphology ( J:100400 )

♀ • ovaries are less dilated than in wild-type mice

♀ • vascularization of ovaries is reduced