Phenotypes associated with this allele

• Allele Symbol: Nras^tm1Rak
• Allele Name: targeted mutation 1, Raju Kucherlapati
• Allele ID: MGI:2385944
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nras^tm1Rak/Nras^tm1Rak	involves: 129P2/OlaHsd * C57BL/6	MGI:3036349
cx2	Hras^tm1Esn/Hras^tm1Esn Nras^tm1Rak/Nras^tm1Rak	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * C57BL/6N	MGI:3036364
cx3	Kras^tm1Tyj/Kras^tm1Tyj Nras^tm1Rak/Nras^+	involves: 129/Sv * C57BL/6	MGI:3589358
cx4	Kras^tm1Tyj/Kras^+ Nras^tm1Rak/Nras^tm1Rak	involves: 129/Sv * C57BL/6	MGI:3589202
cx5	Kras^tm1Tyj/Kras^tm1Tyj Nras^tm1Rak/Nras^tm1Rak	involves: 129/Sv * C57BL/6	MGI:3589289

Genotype
MGI:3036349

hm1

• Allelic Composition: Nras^tm1Rak/Nras^tm1Rak
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:23318 )

• viable and fertile; no gross morphological or histological abnormalities, or defects in peripheral blood cell populations were detected in homozygous mice

Genotype
MGI:3036364

cx2

• Allelic Composition: Hras^tm1Esn/Hras^tm1Esn; Nras^tm1Rak/Nras^tm1Rak
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * C57BL/6N

normal phenotype

no abnormal phenotype detected ( J:67374 )

• mice grew normally, were fertile and did not exhibit any obvious phenotype; however, fewer than the expected number of adult compound homozygous mice were obtained in crosses

Genotype
MGI:3589358

cx3

• Allelic Composition: Kras^tm1Tyj/Kras^tm1Tyj; Nras^tm1Rak/Nras⁺
• Genetic Background: involves: 129/Sv * C57BL/6

mortality/aging

embryonic lethality, incomplete penetrance ( J:43433 )

• ~40% of mutant embryos are found dead at E9.5

Genotype
MGI:3589202

cx4

• Allelic Composition: Kras^tm1Tyj/Kras⁺; Nras^tm1Rak/Nras^tm1Rak
• Genetic Background: involves: 129/Sv * C57BL/6

mortality/aging

postnatal lethality, complete penetrance ( J:43433 )

• a few mutant embryos survive up to P2, but are subsequently neglected by their mothers and die shortly thereafter

perinatal lethality, incomplete penetrance ( J:43433 )

• ~30% of these mutants die perinatally

embryonic lethality during organogenesis, incomplete penetrance ( J:43433 )

• ~70% of these mutants die between E10.0 and E12.0

cardiovascular system

distended pericardium ( J:43433 )

• at E10.5, mutant embryos display dilated pericardial sacs

dilated heart ( J:43433 )

• at E10.5, mutant embryos exhibit a dilated heart

embryo

increased embryonic tissue cell apoptosis ( J:43433 )

• by E10.5, cell death extends throughout the entire embryo

embryonic growth arrest ( J:43433 )

• at E10.5, mutant embryos appear to have arrested at ~E9.5

• ~30% of abnormal mutant embryos survive beyond this early developmental block but are readily identifiable up until the final stages of gestation

embryonic growth retardation ( J:43433 )

• by E10.5, 65% of viable mutant embryos appear grossly abnormal

• starting at E9.5, ~50% of mutants are delayed by 0.5-1.0 developmental days, but never show the non-coordinate delay observed in late Kras^tm1Tyj embryos

absent visceral yolk sac blood islands ( J:43433 )

• at E10.5, mutant embryos display a near or complete absence of blood islands in yolk sacs

excessive folding of visceral yolk sac ( J:43433 )

• at E10.5, mutant yolk sacs appear rough and wrinkled

pale yolk sac ( J:43433 )

abnormal embryonic hematopoiesis ( J:43433 )

• at E10.5, mutant embryos exhibit significantly reduced numbers of circulating primitive erythrocytes in either the yolk sac or the embryo

homeostasis/metabolism

hydrops fetalis ( J:43433 )

• at E15.5, viable mutant embryos are developmentally delayed and severely edematous

cellular

increased apoptosis ( J:43433 )

• at E9.5, mutant embryos display prominent cell death in the forebrain, and to a lesser extent along the neural axis

increased embryonic tissue cell apoptosis ( J:43433 )

• by E10.5, cell death extends throughout the entire embryo

hematopoietic system

abnormal embryonic hematopoiesis ( J:43433 )

• at E10.5, mutant embryos exhibit significantly reduced numbers of circulating primitive erythrocytes in either the yolk sac or the embryo

abnormal erythropoiesis ( J:43433 )

• at E15.5-16.5, mutant fetal livers display a significantly reduced ratio in the number of erythroblasts to hepatocytes

anemia ( J:43433 )

• at E15.5, viable mutant embryos are developmentally delayed and severely anemic

• anemia appears to be attributable to inadequate, but apparently normal, production of definitive erythrocytes and may reflect a defect in the survival or differentiation of either the erythroblasts or a more primitive progenitor cells

vision/eye

abnormal eye development ( J:43433 )

• 22% of E13.5-18.5 mutant embryos display an asymmetrical pattern in eye development, involving only the right eye

• either the right eye is significantly smaller than its wild-type counterpart or the pigment of the eye is overgrown

limbs/digits/tail

short tail ( J:43433 )

• 20% of E13.5-18.5 mutant embryos exhibit severe shortening of the tail

liver/biliary system

abnormal hepatocyte morphology ( J:43433 )

• by 16.5, a high % of mutant hepatocytes appear extremely vacuolated in the absence of increased cell death

growth/size/body

embryonic growth retardation ( J:43433 )

• by E10.5, 65% of viable mutant embryos appear grossly abnormal

• starting at E9.5, ~50% of mutants are delayed by 0.5-1.0 developmental days, but never show the non-coordinate delay observed in late Kras^tm1Tyj embryos

integument

pallor ( J:43433 )

• at E10.5, mutant embryos are significantly paler than wild-type embryos

Genotype
MGI:3589289

cx5

• Allelic Composition: Kras^tm1Tyj/Kras^tm1Tyj; Nras^tm1Rak/Nras^tm1Rak
• Genetic Background: involves: 129/Sv * C57BL/6

mortality/aging

embryonic lethality, complete penetrance ( J:43433 )

• double homozygous mutant blastocyst stage embryos are recovered at approximately the expected frequency of 6%

• no viable double mutant embryos are recovered at E9.5