Phenotypes associated with this allele

• Allele Symbol: Tcrd-V1^tm1Kjk
• Allele Name: targeted mutation 1, Kenji Kishihara
• Allele ID: MGI:2385954
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tcrd-V1^tm1Kjk/Tcrd-V1^tm1Kjk	B6.129P2-Tcrd-V1^tm1Kjk	MGI:3818335
hm2	Tcrd-V1^tm1Kjk/Tcrd-V1^tm1Kjk	involves: 129P2/OlaHsd * C57BL/6	MGI:3818333

Genotype
MGI:3818335

hm1

• Allelic Composition: Tcrd-V1^tm1Kjk/Tcrd-V1^tm1Kjk
• Genetic Background: B6.129P2-Tcrd-V1^tm1Kjk

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal T cell receptor delta chain V(D)J recombination ( J:128525 )

• gamma-delta T cells found in lung epithelia preferentially express Vdelta4 instead of Vdelta1 as occurs in wild-type

• this Vdelta4 expression predominates at 4 weeks of age with irregular expression of the other Vdelta segments occurring less frequently than in wild-type mice

• the Vdelta recombination with J segments has limited diversity with 74% expressing a GGIR motif compared to just 23% in wild-type lung epithelial gamma delta T cells

• Vdelta usage of these lung epithelial gamma delta T cells becomes more diverse as mice age

decreased gamma-delta intraepithelial T cell number ( J:128525 )

• the number of gamma-delta T cells found in lung epithelia is about half that of wild-type at 4 weeks of age though no significant differences are observed in older mice (6-20 weeks of age)

hematopoietic system

abnormal T cell receptor delta chain V(D)J recombination ( J:128525 )

• gamma-delta T cells found in lung epithelia preferentially express Vdelta4 instead of Vdelta1 as occurs in wild-type

• this Vdelta4 expression predominates at 4 weeks of age with irregular expression of the other Vdelta segments occurring less frequently than in wild-type mice

• the Vdelta recombination with J segments has limited diversity with 74% expressing a GGIR motif compared to just 23% in wild-type lung epithelial gamma delta T cells

• Vdelta usage of these lung epithelial gamma delta T cells becomes more diverse as mice age

decreased gamma-delta intraepithelial T cell number ( J:128525 )

• the number of gamma-delta T cells found in lung epithelia is about half that of wild-type at 4 weeks of age though no significant differences are observed in older mice (6-20 weeks of age)

Genotype
MGI:3818333

hm2

• Allelic Composition: Tcrd-V1^tm1Kjk/Tcrd-V1^tm1Kjk
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal T cell receptor delta chain V(D)J recombination ( J:64863 )

• there is no recombination with the Vdelta-1 segment

• Vdelta6-Jdelta1 recombination is common within mature DETC and fetal thymocytes in contrast to the Vdelta1-Jdelta1 recombination seen in wild-type DETC

• Vdelta7-Jdelta1 recombination is also observed within fetal thymocytes while rarely being observed in wild-type

abnormal gamma-delta T cell differentiation ( J:64863 )

• the percentage and number of gamma-delta TCR⁺ thymocytes are markedly reduced during the early fetal period (E15-E18)

• the numbers are 20-fold less at E15 and E16, and 4-fold less at E18

• numbers of Vgamma5⁺ thymocytes are reduced even further compared to controls, with nearly 60 times less on E15, 30 times less on E16, and 10 times less on E18

• numbers of Vgamma5⁺ thymocytes are reduced in newborns compared to wild-type

abnormal mature gamma-delta T cell morphology ( J:64863 )

• Vgamma5^- DETC are observed in the epidermis while virtually all wild-type DETC are Vgamma5⁺

• many of these Vgamma5^- DETC are found in clusters, and have a less dendritic shape and lower TCR expression than their Vgamma5⁺ DETC counterparts

decreased gamma-delta intraepithelial T cell number ( J:64863 )

• there is a 22% reduction at 8 weeks of age and a 14% reduction at 16 weeks of age in the number of dendritic epithelial T cells (DETC) found in ear epidermis

• Vgamma5^- DETC are observed in the epidermis while virtually all wild-type DETC are Vgamma5⁺

• only half of the DETC in mice 8 weeks of age express Vgamma5 with 20% of the DETC expressing Vgamma1

• Vgamma5⁺ DETC numbers increase as these mice age with almost a doubling between 1 and 8 weeks of age and almost tripling between 1 and 16 weeks of age

hematopoietic system

abnormal T cell receptor delta chain V(D)J recombination ( J:64863 )

• there is no recombination with the Vdelta-1 segment

• Vdelta6-Jdelta1 recombination is common within mature DETC and fetal thymocytes in contrast to the Vdelta1-Jdelta1 recombination seen in wild-type DETC

• Vdelta7-Jdelta1 recombination is also observed within fetal thymocytes while rarely being observed in wild-type

abnormal gamma-delta T cell differentiation ( J:64863 )

• the percentage and number of gamma-delta TCR⁺ thymocytes are markedly reduced during the early fetal period (E15-E18)

• the numbers are 20-fold less at E15 and E16, and 4-fold less at E18

• numbers of Vgamma5⁺ thymocytes are reduced even further compared to controls, with nearly 60 times less on E15, 30 times less on E16, and 10 times less on E18

• numbers of Vgamma5⁺ thymocytes are reduced in newborns compared to wild-type

abnormal mature gamma-delta T cell morphology ( J:64863 )

• Vgamma5^- DETC are observed in the epidermis while virtually all wild-type DETC are Vgamma5⁺

• many of these Vgamma5^- DETC are found in clusters, and have a less dendritic shape and lower TCR expression than their Vgamma5⁺ DETC counterparts

decreased gamma-delta intraepithelial T cell number ( J:64863 )

• there is a 22% reduction at 8 weeks of age and a 14% reduction at 16 weeks of age in the number of dendritic epithelial T cells (DETC) found in ear epidermis

• Vgamma5^- DETC are observed in the epidermis while virtually all wild-type DETC are Vgamma5⁺

• only half of the DETC in mice 8 weeks of age express Vgamma5 with 20% of the DETC expressing Vgamma1

• Vgamma5⁺ DETC numbers increase as these mice age with almost a doubling between 1 and 8 weeks of age and almost tripling between 1 and 16 weeks of age