Phenotypes associated with this allele

• Allele Symbol: Pou2af1^tm1Pjln
• Allele Name: targeted mutation 1, Peter J Nielsen
• Allele ID: MGI:2386101
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pou2af1^tm1Pjln/Pou2af1^tm1Pjln	C57BL/6-Pou2af1^tm1Pjln	MGI:4354499
hm2	Pou2af1^tm1Pjln/Pou2af1^tm1Pjln	involves: C57BL/6	MGI:4354502
cx3	Pou2af1^tm1Pjln/Pou2af1^tm1Pjln Tg(tetO-Pou2af1)1Pjln/0 Tg(tTA)1Pjln/0	involves: C57BL/6	MGI:4354504

Genotype
MGI:4354499

hm1

• Allelic Composition: Pou2af1^tm1Pjln/Pou2af1^tm1Pjln
• Genetic Background: C57BL/6-Pou2af1^tm1Pjln

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased B cell number ( J:37085 )

• the number of B cells among splenocytes is reduced 30% compared to in wild-type mice

• the number of B cells in lymph nodes is decreased compared to in wild-type mice

spleen hypoplasia ( J:37085 )

• the number of splenocytes is decreased 50% to 70% compared to in wild-type mice

absent spleen germinal center ( J:37085 )

• mice lack well-developed splenic germinal centers unlike in wild-type mice

• however, mice possess primary follicles

decreased IgA level ( J:37085 )

• in unimmunized mice

decreased IgG1 level ( J:37085 )

• in unimmunized mice

decreased IgG2a level ( J:37085 )

• in unimmunized mice

decreased IgG2b level ( J:37085 )

• in unimmunized mice

decreased IgG3 level ( J:37085 )

• in unimmunized mice

hematopoietic system

decreased B cell number ( J:37085 )

• the number of B cells among splenocytes is reduced 30% compared to in wild-type mice

• the number of B cells in lymph nodes is decreased compared to in wild-type mice

spleen hypoplasia ( J:37085 )

• the number of splenocytes is decreased 50% to 70% compared to in wild-type mice

absent spleen germinal center ( J:37085 )

• mice lack well-developed splenic germinal centers unlike in wild-type mice

• however, mice possess primary follicles

decreased IgA level ( J:37085 )

• in unimmunized mice

decreased IgG1 level ( J:37085 )

• in unimmunized mice

decreased IgG2a level ( J:37085 )

• in unimmunized mice

decreased IgG2b level ( J:37085 )

• in unimmunized mice

decreased IgG3 level ( J:37085 )

• in unimmunized mice

Genotype
MGI:4354502

hm2

• Allelic Composition: Pou2af1^tm1Pjln/Pou2af1^tm1Pjln
• Genetic Background: involves: C57BL/6

immune system

increased B cell apoptosis ( J:67362 )

• among B220^lo B cells in the bone marrow

abnormal B cell differentiation ( J:67362 )

• fewer transitional immature cells are found in the bone marrow due to inefficient development and survival and result in a decrease in these cells in the spleen unlike in wild-type mice

• B cell production rate is 20-fold lower than in wild-type mice

• bone marrow fails to reconstitute irradiated wild-type mice resulting in decreased number of transitional immature B cells, decreased mature recirculating B cells in the bone marrow and a 2- to 3-fold reduction in splenic B cells

decreased immature B cell number ( J:67362 )

• splenic B220+ and PB493+ transitional immature B cells are reduced 3-fold compared to in wild-type mice

• immature and transitional immature B cells in the bone marrow are decreased compared to in wild-type mice

• bone marrow fails to reconstitute irradiated wild-type mice resulting in decreased number of transitional immature B cells

decreased B cell number ( J:67362 , J:123039 )

• mature recirculating B cells in the bone marrow are greatly reduced compared to in wild-type mice (J:67362)

• bone marrow fails to reconstitute irradiated wild-type mice resulting in decreased number of mature recirculating B cells in the bone marrow (J:67362)

• peripheral B cells are decreased compared to in wild-type mice (J:123039)

decreased mature B cell number ( J:67362 )

• mature recirculating B cells in the bone marrow are greatly reduced compared to in wild-type mice

• bone marrow fails to reconstitute irradiated wild-type mice resulting in decreased number of mature recirculating B cells in the bone marrow

decreased T cell number ( J:123039 )

• 2-fold

abnormal T-helper 1 physiology ( J:123039 )

• polarized and restimulated T helper cells produce less IL2 and IFN-gamma than similarly treated wild-type cells

abnormal T-helper 2 physiology ( J:123039 )

• polarized and restimulated T helper cells produce more IL4 and IL13 than similarly treated wild-type cells

decreased interferon-gamma secretion ( J:123039 )

• after restimulation with L. major, mice fail to produce IFN-gamma unlike similarly treated wild-type mice

• polarized and restimulated T helper cells produce less IFN-gamma than similarly treated wild-type cells due fewer cells producing IFN-gamma and less IFN-gamma produced per cell

increased interleukin-13 secretion ( J:123039 )

• after restimulation with L. major, mice produce slightly more IL13 compared with similarly treated wild-type mice

• polarized and restimulated T helper cells produce more IL13 than similarly treated wild-type cells

decreased interleukin-2 secretion ( J:123039 )

• polarized and restimulated T helper cells produce less IL2 than similarly treated wild-type cells

increased interleukin-4 secretion ( J:123039 )

• polarized and restimulated T helper cells produce more IL4 than similarly treated wild-type cells due more cells producing IL4 and more IL4 produced per cell

increased susceptibility to parasitic infection ( J:123039 )

• mice infected with L. major exhibit increased footpad swelling and 20- to 100-fold higher parasitic load in the infected footpad and popliteal draining lymph nodes compared with similarly treated wild-type mice

• after restimulation with L. major, mice fail to produce IFN-gamma while IL13 production is slightly increased compared with similarly treated wild-type mice

• L. major-infected mice exhibit reduced T and B cells compared with similarly treated wild-type mice

• however, dendritic cell and macrophage response to L. major infection is normal

• Rag2 null mice reconstituted with CD4T T cells exhibit increased susceptibility to L. major infection compared with mice reconstituted with wild-type CD4+ T cells

hematopoietic system

increased B cell apoptosis ( J:67362 )

• among B220^lo B cells in the bone marrow

abnormal B cell differentiation ( J:67362 )

• fewer transitional immature cells are found in the bone marrow due to inefficient development and survival and result in a decrease in these cells in the spleen unlike in wild-type mice

• B cell production rate is 20-fold lower than in wild-type mice

• bone marrow fails to reconstitute irradiated wild-type mice resulting in decreased number of transitional immature B cells, decreased mature recirculating B cells in the bone marrow and a 2- to 3-fold reduction in splenic B cells

decreased immature B cell number ( J:67362 )

• splenic B220+ and PB493+ transitional immature B cells are reduced 3-fold compared to in wild-type mice

• immature and transitional immature B cells in the bone marrow are decreased compared to in wild-type mice

• bone marrow fails to reconstitute irradiated wild-type mice resulting in decreased number of transitional immature B cells

decreased B cell number ( J:67362 , J:123039 )

• mature recirculating B cells in the bone marrow are greatly reduced compared to in wild-type mice (J:67362)

• bone marrow fails to reconstitute irradiated wild-type mice resulting in decreased number of mature recirculating B cells in the bone marrow (J:67362)

• peripheral B cells are decreased compared to in wild-type mice (J:123039)

decreased mature B cell number ( J:67362 )

• mature recirculating B cells in the bone marrow are greatly reduced compared to in wild-type mice

• bone marrow fails to reconstitute irradiated wild-type mice resulting in decreased number of mature recirculating B cells in the bone marrow

decreased T cell number ( J:123039 )

• 2-fold

abnormal T-helper 1 physiology ( J:123039 )

• polarized and restimulated T helper cells produce less IL2 and IFN-gamma than similarly treated wild-type cells

abnormal T-helper 2 physiology ( J:123039 )

• polarized and restimulated T helper cells produce more IL4 and IL13 than similarly treated wild-type cells

cellular

increased B cell apoptosis ( J:67362 )

• among B220^lo B cells in the bone marrow

Genotype
MGI:4354504

cx3

• Allelic Composition: Pou2af1^tm1Pjln/Pou2af1^tm1Pjln; Tg(tetO-Pou2af1)1Pjln/0; Tg(tTA)1Pjln/0
• Genetic Background: involves: C57BL/6

immune system

immune system phenotype ( J:67362 )

N • unlike in Pou2af1^tm1Pjln homozygotes, mice exhibit normal numbers of pro-, pre-, immature, and transitional immature B cells and normal rates of B cell apoptosis

decreased B cell number ( J:67362 )

• in the spleen

decreased mature B cell number ( J:67362 )

decreased immunoglobulin level ( J:67362 )

• serum levels of secondary immunoglobins are greatly reduced compared to in wild-type mice

hematopoietic system

decreased B cell number ( J:67362 )

• in the spleen

decreased mature B cell number ( J:67362 )

decreased immunoglobulin level ( J:67362 )

• serum levels of secondary immunoglobins are greatly reduced compared to in wild-type mice