Analysis Tools
mortality/aging
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• homozygous mutant embryos exhibit no overt defects until E11.0 but die by E12.5
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cardiovascular system
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• at E11.0, fetal blood also hemorrhages into the cephalic ventricles
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• at E11.0, all homozygotes are severely hematorrhachitic: they hemorrhage from the dorsal aorta into the lumen of the neural tube
• at E11.0, all homozygotes show an impaired columnar neuroepithelium, a reduced adjacent extracellular matrix, and a hematoma at the site of hemorrhage
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embryo
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• at E11.0, homozygotes dissected free of the yolk sac are visibly smaller than wild-type
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pale yolk sac
(
J:63242
)
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• at E11.0, all homozygotes show a complete absence of red cells in the yolk sac vasculature
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growth/size/body
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• at E11.0, homozygotes dissected free of the yolk sac are visibly smaller than wild-type
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hematopoietic system
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• cultures of progenitors from mutant yolk sacs show reduced levels of CFU-mix: neutrophils, macrophages, mast cells, and erythroid cells, but not megakaryocytes, are present
• at E11.0, mutant fetal liver cells display defects in hematopoietic colony formation, including a severe reduction in the number of pronormoblasts and basophilic normoblasts
• at E11.0, the predominant cells present in mutant fetal livers are orthochromatic and polychromatophilic normoblasts; some of these display megaloblastoid changes
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• cells from the aorta-gonad-mesonephros region (AGM) of E10.0 homozygotes show a significant reduction in the number of megakaryocytes relative to wild-type
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• AGM cells from E10.0 homozygotes show a significant reduction in the % of pronormoblasts and basophilic normoblasts and contain predominantly orthochromatic and polychromatophilic normoblasts
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liver/biliary system
small liver
(
J:63242
)
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• at E11.0, the developing livers of homozygotes are smaller than those of wild-type
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pale liver
(
J:63242
)
|
• at E11.0, the developing livers of homozygotes appear to be pale
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nervous system
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• at E11.0, fetal blood also hemorrhages into the cephalic ventricles
|
|
• at E11.0, all homozygotes are severely hematorrhachitic: they hemorrhage from the dorsal aorta into the lumen of the neural tube
• at E11.0, all homozygotes show an impaired columnar neuroepithelium, a reduced adjacent extracellular matrix, and a hematoma at the site of hemorrhage
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