Analysis Tools|
Allele Symbol Allele Name Allele ID |
Mmp2tm1Ito targeted mutation 1, Shigeyoshi Itohara MGI:2386252 |
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| Summary |
12 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice exhibit 35% less ischemia-induced retinal neovascularization compared with similarly treated wild-type mice
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• hyperteloric with narrower, taller skulls
• overall skull lengths are decreased and about 10% shorter by 12 weeks of age
• area between the right and left frontal-squamosal intersection at the temporal crest, mid-cranial width, is wider and this difference increases with age
• mid-cranial width differences continue to increase from about 10% at 4 weeks to nearly 25% at 24 weeks
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• coronal sutures are prominent and sclerotic
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• lambdoid sutures are prominent and sclerotic
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• sagittal sutures are prominent and sclerotic
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• lower jaw length is about 10% shorter by 12 weeks of age
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• upper jaw length is about 10% shorter by 12 weeks of age
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• bone marrow has transiently decreased osteoclast numbers
• osteoblast and osteoclast numbers per trabecular bone surface area are decreased by more than half at 4 days of age, but no differences are seen at 4 and 12 weeks of age, indicating a transient decrease
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• femoral condyles and proximal tibiae show cartilage destruction with erosions of cartilage occupied by fibrous tissue and inflammatory cells
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• 6 month old mutants show a slight shortening of long bones
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• progressive loss of bone mineral density; mutants begin to show loss at 5 weeks of age and show a 20% reduction at 10 weeks of age that persists throughout early adulthood, and with more rapid loss after 20 weeks of age
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• 13.4% decrease in bone volume at 24 weeks of age
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• defects in cortical bone formation in 4 day old mutants that appear to mostly resolve by 4 weeks of age
• cortical bone continues to show large areas of woven bone compared to normal lamellar tissue in wild-type bone at 4 and 12 weeks of age
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• bone marrow has transiently decreased osteoblast numbers
• osteoblast and osteoclast numbers per trabecular bone surface area are decreased by more than half at 4 days of age, but no differences are seen at 4 and 12 weeks of age, indicating a transient decrease
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• defects in trabecular bone formation in 4 day old mutants that appear to mostly resolve by 4 weeks of age
• primary spongiosa and trabeculae appear less dense and more scant
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• knee joints of all 12 week old mutants show articular cartilage destruction and erosion of the underlying bone surface resulting in the loss of the smooth tibial and femoral surfaces
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• bone of 4 day old mutants shows overall paucity and chaotic organization of trabecular bone, and marked hypocellularity and ragged metaphyseal and diaphyseal cortical bone, a phenotype seen in earlier embryological time points, indicating retarded bone growth
• stigmata of abnormal cortical growth remains at 4 weeks of age
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• hyperteloric with narrower, taller skulls
• overall skull lengths are decreased and about 10% shorter by 12 weeks of age
• area between the right and left frontal-squamosal intersection at the temporal crest, mid-cranial width, is wider and this difference increases with age
• mid-cranial width differences continue to increase from about 10% at 4 weeks to nearly 25% at 24 weeks
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• coronal sutures are prominent and sclerotic
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• lambdoid sutures are prominent and sclerotic
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• sagittal sutures are prominent and sclerotic
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• lower jaw length is about 10% shorter by 12 weeks of age
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• upper jaw length is about 10% shorter by 12 weeks of age
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• broad snout
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• snouts are about 15% shorter than wild-type at 4 weeks of age
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• broad snout
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• snouts are about 15% shorter than wild-type at 4 weeks of age
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• bone marrow has transiently decreased osteoclast numbers
• osteoblast and osteoclast numbers per trabecular bone surface area are decreased by more than half at 4 days of age, but no differences are seen at 4 and 12 weeks of age, indicating a transient decrease
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• 60% decrease in the number of proliferating cells in the bone marrow at 4 days of age but not at 4 or 12 weeks of age
• bone marrow stromal cells and calvaria are unable to support osteoblast and osteoclast growth ex vivo
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• bone marrow has transiently decreased osteoclast numbers
• osteoblast and osteoclast numbers per trabecular bone surface area are decreased by more than half at 4 days of age, but no differences are seen at 4 and 12 weeks of age, indicating a transient decrease
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• femoral condyles and proximal tibiae show cartilage destruction with erosions of cartilage occupied by fibrous tissue and inflammatory cells
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• mice exhibit 35% less ischemia-induced retinal neovascularization compared with similarly treated wild-type mice
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• intercanthal distances are increased by about 10% at 4 weeks of age, however by 12 weeks of age, they are no longer statistically different
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• delayed alveolar formation, however lungs were indistinguishable from wild-type by P14
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• reduced body size, even at birth
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• growth rate about 15% slower than normal
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• exhibit significantly reduced carotid artery intimal hyperplasia in response to vascular injury and fewer intimal smooth muscle cells
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• reduced neovascularization of implanted melanoma cells
(J:46091)
• thickness of choroidal neovascular membranes was 31% lower than normal after treatment with krypton lasers
(J:81029)
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• left ventricular diameters did not increase as much after infarction as they did in controls
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• better survival in the first 7 days after myocardial infarction than controls (90% survival as compared to 61%)
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• exhibit impairment of smooth muscle cell migration through a gelatin-coated membrane towards a chemoattractant and in a wound assay
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• earlier onset and increased severity of experimental autoimmune encephalomyelitis induced by immunization with myelin oligodendrocyte glycoprotein
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• inflammatory cell infiltration and granulation tissue seen in response to implanted melanoma cells but to a lesser extent than in controls
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• increased T-cell transmigration through the brain endothelium, dependent on the increased expression of Mmp9 seen in lymphocytes
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• reduced tumor development after implantation of melanoma cells
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• increased T-cell transmigration through the brain endothelium, dependent on the increased expression of Mmp9 seen in lymphocytes
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• exhibit impairment of smooth muscle cell migration through a gelatin-coated membrane towards a chemoattractant and in a wound assay
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• better survival in the first 7 days after myocardial infarction than controls (90% survival as compared to 61%)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• homozygous mutants are indistinguishable from wild-type littermates
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• resistance to corneal infection is similar to Timp1 single homozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• following laser trauma, mice exhibit decreased choroidal neovascularization compared with similarly treated single homozygote
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• following laser trauma, mice exhibit decreased choroidal neovascularization compared with similarly treated single homozygote
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Cardiac neutrophil accumulation post-myocardial infraction is normalized in Timp4tm1Vuo/Timp4tm1Vuo Mmp2tm1Ito/Mmp2tm1Ito mice
| N |
• mice exhibit normal mortality and neutrophil accumulation following left anterior descending coronary artery ligation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• angiotensin II infused mutants exhibit a greater disruption of the medial elastic lamellae and fibrillar structures in the abdominal aortic walls than in single Timp3 homozygotes
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• angiotensin II infused mutants exhibit a greater disruption of the medial elastic lamellae and fibrillar structures in the abdominal aortic walls than in single Timp3 homozygotes
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• 75% of mutants develop abdominal aortic aneurysm with greater than 50% aortic dilation in the suprarenal region after 4 weeks of angiotensin II infusion
• survival is compromised more than in single Timp3 homozygotes after angiotensin II infusion due to aortic rupture, with mice showing around 70% survival 28 days after infusion
• treatment with a broad spectrum protease inhibitor, PD166793, during the course of angiotensin II infusion blocks abdominal aortic aneurysm development
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• mutants treated with angiotensin II exhibit heightened inflammation in the abdominal aorta, with enhanced infiltration of neutrophils and macrophages
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• mutants treated with angiotensin II exhibit heightened inflammation in the abdominal aorta, with enhanced infiltration of neutrophils and macrophages
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• survival is compromised more than in single Timp3 homozygotes after angiotensin II infusion due to aortic rupture, with mice showing around 70% survival 28 days after infusion
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 2/3 die by E11 and none survive beyond E11.5, however exhibit partial rescue of the phenotypes seen in single Reck homozygotes such as increased body size and tissue integrity
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• neonates die within 10 minutes of birth
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• the proportion of vessels with a diameter greater than 30 nm is reduced
• many capillaries with minimal luminal opening are seen
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• neonates weigh about 10 - 30% less than Mmp2tm1Ito single homozygotes
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• more muscle fibers appear to be immature with central nuclei present and in vitro myotube formation is impaired
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• the back and intercostal muscles have shorter muscle fibers in an abnormal wavy pattern
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• the diaphragm is consistently thinner in double mutants compared to Mmp2tm1Ito single homozygotes
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• the skull and bones are generally smaller
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• ossification and growth plate vascularization are impaired
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mutants weigh about 20% less than control littermates but are fertile
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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