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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Rasgrp1tm1Jstn
targeted mutation 1, James C Stone
MGI:2386261
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Rasgrp1tm1Jstn/Rasgrp1tm1Jstn involves: 129P3/J MGI:5565191
hm2
 		Rasgrp1tm1Jstn/Rasgrp1tm1Jstn involves: 129P3/J * C57BL/6J MGI:3629771
cx3
 		Rasgrp1tm1Jstn/Rasgrp1tm1Jstn
Rasgrp3tm1Jstn/Rasgrp3tm1Jstn 		involves: 129P3/J * C57BL/6J MGI:3629773


Genotype
MGI:5565191
hm1
Allelic
Composition		 Rasgrp1tm1Jstn/Rasgrp1tm1Jstn
Genetic
Background		 involves: 129P3/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rasgrp1tm1Jstn mutation (1 available); any Rasgrp1 mutation (57 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal double-positive T cell morphology ( J:207898 )
• decreased frequency of CD69hi TCRbetahi CD4+CD8+ double positive thymocytes
abnormal CD4-positive, alpha beta T cell morphology ( J:207898 )
• decreased number of Foxp3+CD4 single positive cells in the thymus

hematopoietic system
abnormal double-positive T cell morphology ( J:207898 )
• decreased frequency of CD69hi TCRbetahi CD4+CD8+ double positive thymocytes
abnormal CD4-positive, alpha beta T cell morphology ( J:207898 )
• decreased number of Foxp3+CD4 single positive cells in the thymus




Genotype
MGI:3629771
hm2
Allelic
Composition		 Rasgrp1tm1Jstn/Rasgrp1tm1Jstn
Genetic
Background		 involves: 129P3/J * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rasgrp1tm1Jstn mutation (1 available); any Rasgrp1 mutation (57 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased thymocyte number ( J:108677 )
• mice have an increase in the proportion of CD138+ cells at 6 weeks
enlarged spleen ( J:108677 )
• at 2-3 months of age, mice develop splenomegaly
increased plasma cell number ( J:108677 )
• mice have an increase in the proportion of CD138+ cells at 6 weeks
decreased B cell number ( J:108677 )
• during in vitro assays where B cells from young mice are co-stimulated with Il4 and anti-IgM, proliferation is lower than in wild-type
abnormal humoral immune response ( J:108677 )
• in T cell-independent responses, mice produced equal or higher antibody titers than wild-type particularly IgG1 and IgG2a levels
increased immunoglobulin level ( J:108677 )
• mice exhibit elevated titers of most Ig isotypes compared to wild-type

hematopoietic system
decreased thymocyte number ( J:108677 )
• mice have an increase in the proportion of CD138+ cells at 6 weeks
enlarged spleen ( J:108677 )
• at 2-3 months of age, mice develop splenomegaly
increased plasma cell number ( J:108677 )
• mice have an increase in the proportion of CD138+ cells at 6 weeks
decreased B cell number ( J:108677 )
• during in vitro assays where B cells from young mice are co-stimulated with Il4 and anti-IgM, proliferation is lower than in wild-type
increased immunoglobulin level ( J:108677 )
• mice exhibit elevated titers of most Ig isotypes compared to wild-type

endocrine/exocrine glands
decreased thymocyte number ( J:108677 )
• mice have an increase in the proportion of CD138+ cells at 6 weeks

growth/size/body
enlarged spleen ( J:108677 )
• at 2-3 months of age, mice develop splenomegaly




Genotype
MGI:3629773
cx3
Allelic
Composition		 Rasgrp1tm1Jstn/Rasgrp1tm1Jstn
Rasgrp3tm1Jstn/Rasgrp3tm1Jstn
Genetic
Background		 involves: 129P3/J * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rasgrp1tm1Jstn mutation (1 available); any Rasgrp1 mutation (57 available)
Rasgrp3tm1Jstn mutation (1 available); any Rasgrp3 mutation (43 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased B cell number ( J:108677 )
• during in vitro assays where B cells from young mice are co-stimulated with Il4 and anti-IgM, proliferation is lower than in wild-type or either single mutant
• B cells show weak proliferation when co-stimulated by anti-IgM and anti-CD40 compared to wild-type or either single mutant while response to anti-IgM, proliferative response to is weaker than wild-type or Rasgrp1-deficient mice
decreased plasma cell number ( J:108677 )
• mice have a lower proportion of CD138+ cells at 6 weeks compared to Rasgrp1-deficient mice
abnormal humoral immune response ( J:108677 )
• in T-cell independent responses, mice showed the greatest defect in T cell-dependent type I induction of IgG1 and IgG2a
• mice also show defective response in T cell-dependent response assays compared to wild-type

hematopoietic system
decreased B cell number ( J:108677 )
• during in vitro assays where B cells from young mice are co-stimulated with Il4 and anti-IgM, proliferation is lower than in wild-type or either single mutant
• B cells show weak proliferation when co-stimulated by anti-IgM and anti-CD40 compared to wild-type or either single mutant while response to anti-IgM, proliferative response to is weaker than wild-type or Rasgrp1-deficient mice
decreased plasma cell number ( J:108677 )
• mice have a lower proportion of CD138+ cells at 6 weeks compared to Rasgrp1-deficient mice




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Send questions and comments to User Support. 		last database update
12/17/2024
MGI 6.24 		The Jackson Laboratory