Analysis Tools
mortality/aging
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• homozygous mutant embryos die before E8.5, prior to gastrulation; resorptions are first evident at E7.5
• at E9.5, most mutant embryos are in resorption; remaining embryos exhibit no distinguishable structures
• no mutant embryos are recovered between E10.5 and E15.5
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embryo
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• homozygotes display defects in early embryo development and patterning as early as E5.5
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• at E5.5, mutant embryos lack proximal-distal polarity
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• mutant embryos do not develop beyond E6.5
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• at E6.5-E9.5, mutant embryos are severely growth-retarded and significantly smaller than wild-type or heterozygous embryos
• the first signs of growth retardation are noted at E5.5
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• no mesoderm is detectable at E6.5
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• at E5.5, the embryonic region has lost its anterior-posterior and proximal-distal polarity
• at E6.5, the embryonic region is severely disorganized
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• at E5.5, the extraembryonic and embryonic regions are separated
• at E6.5, both extraembryonic and embryonic regions are severely disorganized and separated into two independent identities, each with its own proamniotic cavity
• at E7.5, the embryonic and extraembryonic tissues are still separate and become ectopically located
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• at E7.5, the cell layer surrounding the embryonic ectoderm is a continuation of the extraembryonic ectoderm
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• at E6.5, the extraembryonic regions is severely disorganized
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• at E7.5, the extraembryonic region appears to proliferate in an uncontrolled fashion, while the embryonic region remains reduced and disorganized
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• at E5.5 and E6.5, the visceral endoderm is disorganized; only a few fragmented visceral endoderm cells are present which fail to form a continuous layer
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growth/size/body
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• at E6.5-E9.5, mutant embryos are severely growth-retarded and significantly smaller than wild-type or heterozygous embryos
• the first signs of growth retardation are noted at E5.5
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