Phenotypes associated with this allele

• Allele Symbol: Pax5^tm3Mbu
• Allele Name: targeted mutation 3, Meinrad Busslinger
• Allele ID: MGI:2386577
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pax5^tm3Mbu/Pax5^tm3Mbu	involves: 129P2/OlaHsd * C57BL/6	MGI:3720349
cn2	Cd19^tm1(cre)Cgn/Cd19^+ Pax5^tm1Mbu/Pax5^tm3Mbu	involves: 129P2/OlaHsd * 129S2/SvPas	MGI:3720352
cn3	Pax5^tm1Mbu/Pax5^tm3Mbu Tg(Mx1-cre)1Cgn/?	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * CBA	MGI:3720351
cn4	Pax5^tm3Mbu/Pax5^+ Tcf3^tm1(PBX1)Mlc/Tcf3^+ Cd19^tm1(cre)Cgn/Cd19^+	involves: 129P2/OlaHsd * C57BL/6	MGI:5825360
cn5	Pax5^tm3Mbu/Pax5^tm3Mbu Tg(Gad1-cre,-lacZ)1Mini/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:5486579
cn6	Pax5^tm3Mbu/Pax5^+ Tg(Gad1-cre,-lacZ)1Mini/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:5486580

Genotype
MGI:3720349

hm1

• Allelic Composition: Pax5^tm3Mbu/Pax5^tm3Mbu
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:75504 )

• mice were indistinguishable from wild-type mice

Genotype
MGI:3720352

cn2

• Allelic Composition: Cd19^tm1(cre)Cgn/Cd19⁺; Pax5^tm1Mbu/Pax5^tm3Mbu
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas

immune system

abnormal B cell differentiation ( J:75504 )

• LPS-stimulated lymph node B cells exhibit a low frequency of and a delay in increased cell size relative to wild-type cells

abnormal class switch recombination ( J:75504 )

• IgG class switching is decreased

decreased immature B cell number ( J:75504 )

• short-lived IgM-B220^lo and IgM+B220^lo immature B cells are slightly lower in number

decreased mature B cell number ( J:75504 )

• there is a 3-fold reduction in the number of long-lived mature B cells

• mice lack recirculating IgM+IgD+B220^hi cells in the bone marrow

decreased pre-B cell number ( J:75504 )

• short-lived IgM-B220^lo B cells are slightly lower in number

decreased immunoglobulin level ( J:75504 )

• total immunoglobin is reduced 3-fold compared to control mice

decreased IgG level ( J:75504 )

• IgG levels are decreased ranging from a 9.4-fold decrease of IgG1 to a 2-fold decrease in IgG3 levels

decreased IgG1 level ( J:75504 )

decreased IgG3 level ( J:75504 )

hematopoietic system

abnormal B cell differentiation ( J:75504 )

• LPS-stimulated lymph node B cells exhibit a low frequency of and a delay in increased cell size relative to wild-type cells

abnormal class switch recombination ( J:75504 )

• IgG class switching is decreased

decreased immature B cell number ( J:75504 )

• short-lived IgM-B220^lo and IgM+B220^lo immature B cells are slightly lower in number

decreased mature B cell number ( J:75504 )

• there is a 3-fold reduction in the number of long-lived mature B cells

• mice lack recirculating IgM+IgD+B220^hi cells in the bone marrow

decreased pre-B cell number ( J:75504 )

• short-lived IgM-B220^lo B cells are slightly lower in number

decreased immunoglobulin level ( J:75504 )

• total immunoglobin is reduced 3-fold compared to control mice

decreased IgG level ( J:75504 )

• IgG levels are decreased ranging from a 9.4-fold decrease of IgG1 to a 2-fold decrease in IgG3 levels

decreased IgG1 level ( J:75504 )

decreased IgG3 level ( J:75504 )

Genotype
MGI:3720351

cn3

• Allelic Composition: Pax5^tm1Mbu/Pax5^tm3Mbu; Tg(Mx1-cre)1Cgn/?
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * CBA

immune system

decreased mature B cell number ( J:75504 )

• half of the mature B cells in the spleen are absent

• only a small number of mature B cells are lost upon short-term inactivation of Pax5

• polyinosine-polycystosine (pIpC)-treated mice lack IgD+B220^hi cells

hematopoietic system

decreased mature B cell number ( J:75504 )

• half of the mature B cells in the spleen are absent

• only a small number of mature B cells are lost upon short-term inactivation of Pax5

• polyinosine-polycystosine (pIpC)-treated mice lack IgD+B220^hi cells

Genotype
MGI:5825360

cn4

• Allelic Composition: Pax5^tm3Mbu/Pax5⁺; Tcf3^tm1(PBX1)Mlc/Tcf3⁺; Cd19^tm1(cre)Cgn/Cd19⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

neoplasm

increased B cell acute lymphoblastic leukemia incidence ( J:226241 )

• mice exhibit increased penetrance and accelerated acute leukemia development compared to single Tcf3^tm1(PBX1)Mlc conditional mutants

hematopoietic system

decreased immature B cell number ( J:226241 )

• preleukemic mice show a greater decrease of immature B cells of the Lin-CD19+CD43- fractions compared to Tcf3^tm1(PBX1)Mlc conditional mutants, indicating a more severe block of B cell differentiation at this stage of maturation

abnormal pro-B cell morphology ( J:226241 )

• preleukemic mice show expansion of GFP+ progenitor cells of the Lin-CD19+CD43+ fractions at younger ages compared to single Tcf3^tm1(PBX1)Mlc conditional mutants, indicating accelerated preleukemic progenitor B cell expansion

arrested B cell differentiation ( J:226241 )

• preleukemic mice show a greater decrease of immature B cells of the Lin-CD19+CD43- fractions compared to Tcf3^tm1(PBX1)Mlc conditional mutants, indicating a more severe block of B cell differentiation at this stage of maturation

immune system

decreased immature B cell number ( J:226241 )

• preleukemic mice show a greater decrease of immature B cells of the Lin-CD19+CD43- fractions compared to Tcf3^tm1(PBX1)Mlc conditional mutants, indicating a more severe block of B cell differentiation at this stage of maturation

abnormal pro-B cell morphology ( J:226241 )

• preleukemic mice show expansion of GFP+ progenitor cells of the Lin-CD19+CD43+ fractions at younger ages compared to single Tcf3^tm1(PBX1)Mlc conditional mutants, indicating accelerated preleukemic progenitor B cell expansion

arrested B cell differentiation ( J:226241 )

• preleukemic mice show a greater decrease of immature B cells of the Lin-CD19+CD43- fractions compared to Tcf3^tm1(PBX1)Mlc conditional mutants, indicating a more severe block of B cell differentiation at this stage of maturation

Genotype
MGI:5486579

cn5

• Allelic Composition: Pax5^tm3Mbu/Pax5^tm3Mbu; Tg(Gad1-cre,-lacZ)1Mini/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

mortality/aging

premature death ( J:195054 )

• mice die 1-2 weeks after macroscopic changes appear (ie. domed skulls); life expectancy of such mutants is 58 days; mice that do not show this phenotype survive at least 12 months and are fertile

skeleton

domed cranium ( J:195054 )

• 47% of mice show dome shaped skulls by about postnatal day 50

craniofacial

domed cranium ( J:195054 )

• 47% of mice show dome shaped skulls by about postnatal day 50

behavior/neurological

increased anxiety-related response ( J:195054 )

• mutants spend less time in the center in open-field tests, suggesting increased anxiogenic-like behavior

increased locomotor activity ( J:195054 )

• in a novel open-field test, mutants with dome-shaped heads show significantly increased locomotion (likely due to the novelty aspect), as mutants appear lethargic in the home cages

nervous system

hydrocephaly ( J:195054 )

• 47% of mice

dilated lateral ventricle ( J:195054 )

• prominent, varying mild to severe

• in most severe cases, medial structures between ventricles are deformed and compressed, as represented by vertically relocated hippocampi

• ventricular dilation might be result of obstruction of interventricular connections, increased CSF production by ependymal cells of the choroid plexus, or decreased CSF resorption by arachnoid villi

• other ventricles do not show enlargement

abnormal cerebral cortex morphology ( J:195054 )

• cortex surrounding lateral ventricles is markedly thinner than in controls

Genotype
MGI:5486580

cn6

• Allelic Composition: Pax5^tm3Mbu/Pax5⁺; Tg(Gad1-cre,-lacZ)1Mini/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

mortality/aging

premature death ( J:195054 )

• mice die 1-2 weeks after macroscopic changes appear (ie. domed skulls); life expectancy of such mutants is 58 days; mice that do not show this phenotype survive at least 12 months and are fertile

skeleton

domed cranium ( J:195054 )

• 19% of mice show dome shaped skulls by about postnatal day 50

craniofacial

domed cranium ( J:195054 )

• 19% of mice show dome shaped skulls by about postnatal day 50

behavior/neurological

increased anxiety-related response ( J:195054 )

• mutants spend less time in the center in open-field tests, suggesting increased anxiogenic-like behavior

increased locomotor activity ( J:195054 )

• in a novel open-field test, mutants with dome-shaped heads show significantly increased locomotion (likely due to the novelty aspect), as mutants appear lethargic in the home cages

nervous system

hydrocephaly ( J:195054 )

• 19% of mice

dilated lateral ventricle ( J:195054 )

• prominent, varying mild to severe