Phenotypes associated with this allele

• Allele Symbol: Stat6^tm1Jni
• Allele Name: targeted mutation 1, James N Ihle
• Allele ID: MGI:2386745
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Stat6^tm1Jni/Stat6^tm1Jni	involves: 129S/SvEv	MGI:3844243
hm2	Stat6^tm1Jni/Stat6^tm1Jni	involves: 129S/SvEv * C57BL/6	MGI:3844280
cx3	Stat4^tm1Jni/Stat4^tm1Jni Stat6^tm1Jni/Stat6^tm1Jni	involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6	MGI:3844304

Genotype
MGI:3844243

hm1

• Allelic Composition: Stat6^tm1Jni/Stat6^tm1Jni
• Genetic Background: involves: 129S/SvEv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal T-helper 1 cell differentiation ( J:32607 )

• T cells cultured in vitro under Th2-polarizing conditions including IL-4 keep producing IFN-gamma suggesting a lack of suppression of Th1 differentiation

abnormal T-helper 2 cell differentiation ( J:32607 )

• T cells cultured in vitro under Th2-polarizing conditions fail to differentiate into Th2 effector cells

abnormal B cell physiology ( J:32607 )

• when stimulated in the presence of IL-4, mutant B cells proliferate less and have less surface expression of MHC-II, Thy-1, and CD23 compared to controls

• incubation alone with IL-4 upregulates surface expression of CD23 on wild-type but not mutant B cells

increased B cell apoptosis ( J:146533 )

• in response to irradiation despite IL4 treatment

abnormal B cell proliferation ( J:32607 )

• B cells only have half the rate of proliferation as controls when stimulated in the presence of IL-4

abnormal class switch recombination ( J:32607 )

• mice injected with anti-IgD immunoglobulins fail to produce IgE while the same treatment in wild-type mice leads to dramatic increases in IgE

• anti-IgD treated mutant mice have higher levels of IgG2a, IgG2b, and IgG3 than controls

• increases in IgG1 after anti-IgD treatment are similar between mutant and wild-type mice

abnormal level of surface class II molecules ( J:32607 )

• B cells express much less MHC-II on their surface when activated in the presence of IL-4

hematopoietic system

abnormal T-helper 1 cell differentiation ( J:32607 )

• T cells cultured in vitro under Th2-polarizing conditions including IL-4 keep producing IFN-gamma suggesting a lack of suppression of Th1 differentiation

abnormal T-helper 2 cell differentiation ( J:32607 )

• T cells cultured in vitro under Th2-polarizing conditions fail to differentiate into Th2 effector cells

abnormal B cell physiology ( J:32607 )

• when stimulated in the presence of IL-4, mutant B cells proliferate less and have less surface expression of MHC-II, Thy-1, and CD23 compared to controls

• incubation alone with IL-4 upregulates surface expression of CD23 on wild-type but not mutant B cells

increased B cell apoptosis ( J:146533 )

• in response to irradiation despite IL4 treatment

abnormal B cell proliferation ( J:32607 )

• B cells only have half the rate of proliferation as controls when stimulated in the presence of IL-4

abnormal class switch recombination ( J:32607 )

• mice injected with anti-IgD immunoglobulins fail to produce IgE while the same treatment in wild-type mice leads to dramatic increases in IgE

• anti-IgD treated mutant mice have higher levels of IgG2a, IgG2b, and IgG3 than controls

• increases in IgG1 after anti-IgD treatment are similar between mutant and wild-type mice

cellular

increased cellular sensitivity to gamma-irradiation ( J:146533 )

• decreased B cell apoptosis despite IL4 treatment

increased B cell apoptosis ( J:146533 )

• in response to irradiation despite IL4 treatment

abnormal B cell proliferation ( J:32607 )

• B cells only have half the rate of proliferation as controls when stimulated in the presence of IL-4

Genotype
MGI:3844280

hm2

• Allelic Composition: Stat6^tm1Jni/Stat6^tm1Jni
• Genetic Background: involves: 129S/SvEv * C57BL/6

immune system

abnormal T-helper 1 cell differentiation ( J:51096 )

• mice infected with L. mexicana develop an enhanced Th1 response as evidenced by increased IL-2, IL-12 and IFN-gamma secretion

abnormal mast cell morphology ( J:110429 )

• N. brasiliensis infection leads to high numbers of intestinal mucosal mast cells

decreased IgE level ( J:51096 )

• mice produce significantly less IgE in response to L. mexicana infection

• L. mexicana-specific IgE is three-fold less than controls

increased IgE level ( J:110429 )

• N. brasiliensis infection leads to higher levels of IgE than in infected-controls

• higher IgE levels might be due to the chronic infection that occurs in these mice as opposed to the acute infection that occurs in controls

decreased IgG1 level ( J:51096 )

• mice produce 2-log fold less IgG1 in response to L. mexicana infection

increased IgG1 level ( J:110429 )

• N. brasiliensis infection leads to higher levels of IgG1 than in infected-controls

• higher IgG1 levels might be due to the chronic infection that occurs in these mice as opposed to the acute infection that occurs in controls

increased IgG2a level ( J:51096 )

• mice produce 2-log fold more IgG2a in response to L. mexicana infection

increased interferon-gamma secretion ( J:51096 , J:96338 )

• lymphocytes stimulated with L. mexicana antigen produce vastly more IFN-gamma than wild-type controls (J:51096)

• restimulation of lymphocytes from Ova-immunized mice leads to enhanced IFN-gamma secretion (J:96338)

increased interleukin-12 secretion ( J:51096 )

• lymphocytes stimulated with L. mexicana antigen produce 5-fold more IL-12 than wild-type controls

decreased interleukin-4 secretion ( J:96338 )

• restimulation of lymphocytes from Ova-immunized mice fails to solicit an IL-4 response

decreased susceptibility to autoimmune diabetes ( J:96338 )

• mice exhibit a milder form of low dose streptozotocin (STZ)-induced diabetes with blood glucose levels that lower than those of controls

increased susceptibility to parasitic infection ( J:110429 )

• 15 days after inoculation with N. brasiliensis, intestines contain large numbers of adult worms while controls have expelled the worms by this time point

hematopoietic system

abnormal T-helper 1 cell differentiation ( J:51096 )

• mice infected with L. mexicana develop an enhanced Th1 response as evidenced by increased IL-2, IL-12 and IFN-gamma secretion

abnormal mast cell morphology ( J:110429 )

• N. brasiliensis infection leads to high numbers of intestinal mucosal mast cells

decreased IgE level ( J:51096 )

• mice produce significantly less IgE in response to L. mexicana infection

• L. mexicana-specific IgE is three-fold less than controls

increased IgE level ( J:110429 )

• N. brasiliensis infection leads to higher levels of IgE than in infected-controls

• higher IgE levels might be due to the chronic infection that occurs in these mice as opposed to the acute infection that occurs in controls

decreased IgG1 level ( J:51096 )

• mice produce 2-log fold less IgG1 in response to L. mexicana infection

increased IgG1 level ( J:110429 )

• N. brasiliensis infection leads to higher levels of IgG1 than in infected-controls

• higher IgG1 levels might be due to the chronic infection that occurs in these mice as opposed to the acute infection that occurs in controls

increased IgG2a level ( J:51096 )

• mice produce 2-log fold more IgG2a in response to L. mexicana infection

integument

skin lesions ( J:51096 )

• mice fail to develop significant skin lesions in response to Leishmania mexicana infection

• some inflammatory foci comprised primarily of lymphocytes and macrophages and only a few parasites are visible in mutant mice compared to tissue destruction with diffuse inflammatory infiltrate consisting of heavily parasitized macrophages, eosinophils, and lymphocytes

Genotype
MGI:3844304

cx3

• Allelic Composition: Stat4^tm1Jni/Stat4^tm1Jni; Stat6^tm1Jni/Stat6^tm1Jni
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6

immune system

increased interferon-gamma secretion ( J:96338 )

• restimulation of lymphocytes from Ova-immunized mice leads to enhanced IFN-gamma secretion

decreased interleukin-4 secretion ( J:96338 )

• restimulation of lymphocytes from Ova-immunized mice fails to solicit an IL-4 response

increased susceptibility to autoimmune diabetes ( J:96338 )

• mice have a median diabetes-free survival of 7 days after low dose streptozotocin injection compared to 9 days for wild-type controls

• blood glucose levels are significantly higher than controls throughout the entire observation time compared to wild-type littermates