Analysis Tools
mortality/aging
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• homozygous mutant embryos start dying at E12.5; no live mutant embryos are identified past E14.5
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growth/size/body
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• by E13.5, mutant embryos are noticeably growth-retarded relative to wild-type embryos
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liver/biliary system
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• at E13.5, mutant fetal livers exhibit a significant increase in apoptotic hepatocytes; in contrast, the apoptotic cells identified in wild-type livers are primarily composed of the myeloid lineage
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• at E13.5, mutant livers exhibit reduced growth, as shown as reduced BrdU incorporation
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small liver
(
J:59916
)
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• at E13.5, mutant livers are significantly reduced in size relative to wild-type livers
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• at E13.5, total liver cell counts are 15% of wild-type livers
• by E14.5, mutant livers display reduced cellularity and increased empty space
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hematopoietic system
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• mutant embryos become anemic after E11.5 as hematopoiesis switches from the yolk sac to the fetal liver
• by E14.5, the total blood counts of surviving mutants are 20% of the values found in wild-type embryos
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• at E13.5, mutant erythroid cells are predominantly immature, nucleated cells of yolk sac origin, whereas wild-type erythroid cells are 80% liver-derived nonnucleated cells
• notably, mutant hematopoietic progenitors display no cell-autonomous defect in differentiation
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embryo
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• by E13.5, mutant embryos are noticeably growth-retarded relative to wild-type embryos
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integument
cellular
|
• at E13.5, mutant fetal livers exhibit a significant increase in apoptotic hepatocytes; in contrast, the apoptotic cells identified in wild-type livers are primarily composed of the myeloid lineage
|
|
• at E13.5, mutant livers exhibit reduced growth, as shown as reduced BrdU incorporation
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