Analysis Tools|
Allele Symbol Allele Name Allele ID |
Chek2tm1Mak targeted mutation 1, Tak Mak MGI:2386905 |
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| Summary |
9 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mouse embryonic fibroblast cells fail to undergo apoptosis following exposure to ionizing radiation as do wild-type cells
• however, mouse embryonic fibroblast cells arrest in G1 phase following exposure to ionizing radiation unlike Trp53 null cells and similar to wild-type cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• incidence of 7,12-dimethylbenzanthracene (DMBA)-induced skin tumors is enhanced, however mutants do not develop spontaneous tumors by 1 year of age
• onset of DMBA-induced skin tumors occurs earlier than in wild-type
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• mouse embryonic fibroblasts (MEFs) fail to arrest at G1 at low ionizing radiation (IR) doses but behave normally at higher doses
• mutants exposed to IR radiation exhibit a shorter duration of the G2/M checkpoint in the epidermis than wild-type, with mitosis resuming by 24 hours post-IR
• gamma-irradiated thymic cells show a partial defect in G1/S-phase checkpoint, with a milder G1 arrest than observed in Trp53 and Atm mutants
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• cells from thymus, CNS, hair follicles, and fibroblasts are resistant to gamma irradiation-induced apoptosis
• unlike wild-type epidermal cells that show a reduction in proliferation 24 hours post IR irradiation, mutant epidermal cells exhibit no reduction in proliferation
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• incidence of 7,12-dimethylbenzanthracene (DMBA)-induced skin tumors is enhanced, however mutants do not develop spontaneous tumors by 1 year of age
• onset of DMBA-induced skin tumors occurs earlier than in wild-type
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 32% with lymphomas by 180 days of age
• tumors are restricted to the thymus
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| N |
• complete thymocyte restoration
• T cell numbers in the periphery are restored
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• 32% with lymphomas by 180 days of age
• tumors are restricted to the thymus
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| N |
• resistant to gamma irradiation
• restoration of normal cell cycle arrest of T cells when stimulated with antibody to CD3e or CD3 and Il2
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• increased frequency
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• 32% with lymphomas by 180 days of age
• tumors are restricted to the thymus
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• 32% with lymphomas by 180 days of age
• tumors are restricted to the thymus
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• about 50% develop acinar adenocarcenomas
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• about 50% develop acinar adenocarcenomas
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• about 50% develop acinar adenocarcenomas
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 31 of 41 die by 5 months of age
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• pro-B cell numbers are similar to that in homozygous Rad50tm2Jpt mice
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• double-negative T cell numbers are similar to that in homozygous Rad50tm2Jpt mice
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• macrophage numbers are similar to that in homozygous Rad50tm2Jpt mice
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• pro-B cell numbers are similar to that in homozygous Rad50tm2Jpt mice
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• double-negative T cell numbers are similar to that in homozygous Rad50tm2Jpt mice
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• macrophage numbers are similar to that in homozygous Rad50tm2Jpt mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 13 of 65 die by 5 months of age, however survival time is increased compared to homozygous Rad50tm2Jpt mice, as 52 mutants survive past 5 months of age and none develop anemia
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• 10 of 65 mice develop lymphoma
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| N |
• exhibit hematopoietic rescue of the abnormalities seen in homozygous Rad50tm2Jpt mice, with numbers of double-negative T cells, pro-B cells, and macrophages within 2-fold levels of wild-type levels
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• significant reduction in cortical thickness, as determined in motor and visual cortex sections at P60, similar to that in single Cep63Gt(EUCE0251h11)Hmgu homozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 N |
• mice exhibit normal ovary weight and follicle development
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• marginally
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• with reduced MLH1 focus numbers
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• improved cellularity compared with Zswim7em1Mjn homozygotes
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• marginally
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• reduced litter sizes
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• females produce reduced litters
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• marginally
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• with reduced MLH1 focus numbers
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• improved cellularity compared with Zswim7em1Mjn homozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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N |
• mice exhibit normal ovary weight and follicle development
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• improved cellularity compared with Zswim7em1Mjn homozygotes
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• reduced litter sizes
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• females produce reduced litters
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• improved cellularity compared with Zswim7em1Mjn homozygotes
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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