Phenotypes associated with this allele

• Allele Symbol: Ifnb1^tm1Acgp
• Allele Name: targeted mutation 1, Andrew C G Porter
• Allele ID: MGI:2386955
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ifnb1^tm1Acgp/Ifnb1^tm1Acgp	B6.129P2-Ifnb1^tm1Acgp	MGI:4839935
hm2	Ifnb1^tm1Acgp/Ifnb1^tm1Acgp	involves: 129P2/OlaHsd	MGI:3843261
hm3	Ifnb1^tm1Acgp/Ifnb1^tm1Acgp	involves: 129P2/OlaHsd * C57BL/6	MGI:3843239

Genotype
MGI:4839935

hm1

• Allelic Composition: Ifnb1^tm1Acgp/Ifnb1^tm1Acgp
• Genetic Background: B6.129P2-Ifnb1^tm1Acgp

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal macrophage physiology ( J:136368 )

• macrophages exhibit impaired ability to kill Bacillus anthracis compared with wild-type cells

decreased interleukin-1 beta secretion ( J:136368 )

• following exposure of macrophages to LPS or Bacillus anthracis spores

hematopoietic system

abnormal macrophage physiology ( J:136368 )

• macrophages exhibit impaired ability to kill Bacillus anthracis compared with wild-type cells

Genotype
MGI:3843261

hm2

• Allelic Composition: Ifnb1^tm1Acgp/Ifnb1^tm1Acgp
• Genetic Background: involves: 129P2/OlaHsd

immune system

increased T cell proliferation ( J:86527 )

• lymph node T cells hyperproliferate in response to anti-CD3 and anti-CD28 stimulation

decreased pro-B cell number ( J:86527 )

• there is a decrease in the percentage of pro-B cells found in bone marrow (1.2% versus 1.6%)

decreased neutrophil cell number ( J:86527 )

• the percentage of neutrophils in circulation is reduced from 22.9% in wild-type to 14.6% in these mice

decreased mature B cell number ( J:86527 )

• the percentage of IgM⁺ B cells in circulation is reduced from 7.05% in wild-type to 4.88% in these mice

decreased macrophage cell number ( J:86527 )

• the number of red pulp macrophages found in the spleen are reduced

decreased monocyte cell number ( J:86527 )

• the percentage of monocytes in circulation is reduced from 13.1% in wild-type to 7.3% in these mice

abnormal spleen primary B follicle morphology ( J:86527 )

• B cell follicles are ill-defined with a vague B/T cell interface

decreased tumor necrosis factor secretion ( J:86527 )

• bone marrow derived macrophages produce half or less TNF than controls when stimulated with LPS

• defect can be rescued by exogenous IFNbeta

• in vivo production of TNF is reduced in the B cell follicles of the spleen

• production of TNF by activated CD4⁺ and CD8⁺ T cells is also reduced

neoplasm

increased tumor growth/size ( J:86527 )

• mice inoculated with murine carcinoma line LLC-1 have larger tumors 18 days later compared to controls (mean of 518 mm³ vs. 372 mm³in controls)

hematopoietic system

increased T cell proliferation ( J:86527 )

• lymph node T cells hyperproliferate in response to anti-CD3 and anti-CD28 stimulation

decreased common myeloid progenitor cell number ( J:86527 )

• the number of CFU-GM isolated from bone marrow is about half that of controls

decreased pro-B cell number ( J:86527 )

• there is a decrease in the percentage of pro-B cells found in bone marrow (1.2% versus 1.6%)

decreased erythroid progenitor cell number ( J:86527 )

• the number of BFU-E isolated from bone marrow is slightly less than controls

decreased neutrophil cell number ( J:86527 )

• the percentage of neutrophils in circulation is reduced from 22.9% in wild-type to 14.6% in these mice

decreased mature B cell number ( J:86527 )

• the percentage of IgM⁺ B cells in circulation is reduced from 7.05% in wild-type to 4.88% in these mice

decreased macrophage cell number ( J:86527 )

• the number of red pulp macrophages found in the spleen are reduced

decreased monocyte cell number ( J:86527 )

• the percentage of monocytes in circulation is reduced from 13.1% in wild-type to 7.3% in these mice

abnormal spleen primary B follicle morphology ( J:86527 )

• B cell follicles are ill-defined with a vague B/T cell interface

cellular

increased T cell proliferation ( J:86527 )

• lymph node T cells hyperproliferate in response to anti-CD3 and anti-CD28 stimulation

Genotype
MGI:3843239

hm3

• Allelic Composition: Ifnb1^tm1Acgp/Ifnb1^tm1Acgp
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

decreased circulating interferon-beta level ( J:61048 )

• IFN-beta secretion does not occur

increased susceptibility to Poxviridae infection ( J:61048 )

• mice have increased susceptibility to vaccinia virus

• infection with 10³ PFUs leads to increased weight loss, visible signs of illness and death of one mouse out of six infected compared to little weight loss and no disease symptoms or deaths in controls

• higher titers of virus are detected in the brains and lungs of mutant mice 5 to 7 days after infection with 10³ PFUs

• infection with 10⁴ or 10⁵ PFUs leads to death within 8 days for all mutant mice after rapid weight loss and severe signs of illness

• controls have mild-to intermediate signs of illness with one lethality occurring out of 7 mice infected

homeostasis/metabolism

decreased circulating interferon-beta level ( J:61048 )

• IFN-beta secretion does not occur