Phenotypes associated with this allele

• Allele Symbol: Ptgdr^tm1Sna
• Allele Name: targeted mutation 1, Shuh Narumiya
• Allele ID: MGI:2386956
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptgdr^tm1Sna/Ptgdr^tm1Sna	B6.129P2-Ptgdr^tm1Sna/Sna	MGI:5517579
hm2	Ptgdr^tm1Sna/Ptgdr^tm1Sna	involves: 129P2/OlaHsd * C57BL/6	MGI:3057193
cx3	Galc^twi/Galc^twi Ptgdr^tm1Sna/Ptgdr^tm1Sna	B6.Cg-Galc^twi Ptgdr^tm1Sna	MGI:3624280

Genotype
MGI:5517579

hm1

• Allelic Composition: Ptgdr^tm1Sna/Ptgdr^tm1Sna
• Genetic Background: B6.129P2-Ptgdr^tm1Sna/Sna

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal dermal mast cell morphology ( J:197334 )

• fewer mature secretory granules and less histamine content

decreased mast cell histamine storage ( J:197334 )

• in dermal mast cells

abnormal mast cell physiology ( J:197334 )

• bone marrow derived-mast cells only partially restore passive cutaneous anaphylaxis in Kit^W-sh homozygotes unlike wild-type cells that efficiently rescue the phenotype

decreased mast cell degranulation ( J:197334 )

• following passive cutaneous anaphylaxis induced by IgE antigen challenge, mice exhibit poor ear mast cell degranulation compared with wild-type mice

• dermal mast cells exhibit reduced IgE-antigen-induced degranulation compared with wild-type cells

decreased susceptibility to type I hypersensitivity reaction ( J:197334 )

• following passive cutaneous anaphylaxis induced by IgE antigen challenge, mice exhibit lower vascular leakage and poor ear mast cell degranulation compared with wild-type mice

cardiovascular system

abnormal vascular permeability ( J:197334 )

• following passive cutaneous anaphylaxis induced by IgE antigen challenge, mice exhibit lower vascular leakage compared with wild-type mice

integument

abnormal dermal mast cell morphology ( J:197334 )

• fewer mature secretory granules and less histamine content

hematopoietic system

abnormal dermal mast cell morphology ( J:197334 )

• fewer mature secretory granules and less histamine content

decreased mast cell histamine storage ( J:197334 )

• in dermal mast cells

abnormal mast cell physiology ( J:197334 )

• bone marrow derived-mast cells only partially restore passive cutaneous anaphylaxis in Kit^W-sh homozygotes unlike wild-type cells that efficiently rescue the phenotype

decreased mast cell degranulation ( J:197334 )

• following passive cutaneous anaphylaxis induced by IgE antigen challenge, mice exhibit poor ear mast cell degranulation compared with wild-type mice

• dermal mast cells exhibit reduced IgE-antigen-induced degranulation compared with wild-type cells

cellular

decreased mast cell degranulation ( J:197334 )

• following passive cutaneous anaphylaxis induced by IgE antigen challenge, mice exhibit poor ear mast cell degranulation compared with wild-type mice

• dermal mast cells exhibit reduced IgE-antigen-induced degranulation compared with wild-type cells

Genotype
MGI:3057193

hm2

• Allelic Composition: Ptgdr^tm1Sna/Ptgdr^tm1Sna
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

respiratory system

respiratory system inflammation ( J:61023 )

• after antigen exposure of sensitized mice, airway hyperactivity was little affected by antigen exposure

• less affect on Il-4, Il-5, and Il-13

• less cell infiltration of lungs

• fewer mucus containing cells

immune system

increased CD4-positive, alpha-beta T cell number ( J:243958 )

• MHV-infected mice exhibit higher frequency and numbers of virus-specific CD4 T cells in the brain

• however, virus-specific CD8 T cell numbers in MHV-infected mice are similar to wild-type

increased regulatory T cell number ( J:243958 )

• MHV-infected mice exhibit higher numbers of T regulatory cells

• however, MHV-infected mice do not show differences in the frequency and numbers of macrophages, microglia, or neutrophils in the brain

abnormal macrophage activation involved in immune response ( J:243958 )

• marker analysis indicates that MHV-infected mice exhibit less activated macrophages

decreased microglial cell activation ( J:243958 )

• marker analysis indicates that MHV-infected mice exhibit less activated microglia

increased interleukin-1 beta secretion ( J:243958 )

• levels of secreted IL-1beta are greater in bone marrow macrophages at 12 and 24 hours post MHV A59 infection

brain inflammation ( J:243958 )

• mice infected with MHV show more extensive perivascular inflammation in the brain at 6 and 10 days post infection (dpi) than wild-type mice

respiratory system inflammation ( J:61023 )

• after antigen exposure of sensitized mice, airway hyperactivity was little affected by antigen exposure

• less affect on Il-4, Il-5, and Il-13

• less cell infiltration of lungs

• fewer mucus containing cells

increased susceptibility to Coronaviridae infection ( J:243958 )

• mice intracranially infected with the JHM strain of mouse hepatitis virus (MHV) exhibit weight loss, increased morbidity, and increased viral titers in the brain

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:243958 )

• mice intracranially infected with the JHM strain of mouse hepatitis virus (MHV) exhibit increased mortality from 10 % in controls to about 80%

• mice intracranially infected with the A59 strain of MHV show increased mortality, with 0% survival compared to 70% in wild-type mice

• treatment with an IL-1 receptor antagonist anakinra improves the survival of MHV-infected mice from 10% to about 70%

mortality/aging

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:243958 )

• treatment with an IL-1 receptor antagonist anakinra improves the survival of MHV-infected mice from 10% to about 70%

• mice intracranially infected with the JHM strain of mouse hepatitis virus (MHV) exhibit increased mortality from 10 % in controls to about 80%

• mice intracranially infected with the A59 strain of MHV show increased mortality, with 0% survival compared to 70% in wild-type mice

hematopoietic system

increased CD4-positive, alpha-beta T cell number ( J:243958 )

• MHV-infected mice exhibit higher frequency and numbers of virus-specific CD4 T cells in the brain

• however, virus-specific CD8 T cell numbers in MHV-infected mice are similar to wild-type

increased regulatory T cell number ( J:243958 )

• MHV-infected mice exhibit higher numbers of T regulatory cells

• however, MHV-infected mice do not show differences in the frequency and numbers of macrophages, microglia, or neutrophils in the brain

abnormal macrophage activation involved in immune response ( J:243958 )

• marker analysis indicates that MHV-infected mice exhibit less activated macrophages

decreased microglial cell activation ( J:243958 )

• marker analysis indicates that MHV-infected mice exhibit less activated microglia

nervous system

decreased microglial cell activation ( J:243958 )

• marker analysis indicates that MHV-infected mice exhibit less activated microglia

brain inflammation ( J:243958 )

• mice infected with MHV show more extensive perivascular inflammation in the brain at 6 and 10 days post infection (dpi) than wild-type mice

spongiform encephalopathy ( J:243958 )

• mice infected with MHV show large, multifocal, spongiform lesions in the brainstems by 10 dpi compared to only mild lesions with monocytic infiltration in wild-type mice

Genotype
MGI:3624280

cx3

• Allelic Composition: Galc^twi/Galc^twi; Ptgdr^tm1Sna/Ptgdr^tm1Sna
• Genetic Background: B6.Cg-Galc^twi Ptgdr^tm1Sna

mortality/aging

premature death ( J:108359 )

• lifespan of mutants is 46.2 days

behavior/neurological

ataxia ( J:108359 )

• milder than in Galc^twi single mutants

nervous system

abnormal astrocyte morphology ( J:108359 )

• astrocyte processes are much thinner than in Galc^twi single mutants