Phenotypes associated with this allele

• Allele Symbol: Gclc^tm1Mwl
• Allele Name: targeted mutation 1, Michael W Lieberman
• Allele ID: MGI:2387010
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gclc^tm1Mwl/Gclc^tm1Mwl	involves: 129S7/SvEvBrd * C57BL/6	MGI:3814417
ht2	Gclc^tm1Mwl/Gclc^+	involves: 129S7/SvEvBrd * C57BL/6	MGI:3814418

Genotype
MGI:3814417

hm1

• Allelic Composition: Gclc^tm1Mwl/Gclc^tm1Mwl
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:62177 )

• embryos die between E7.5 and E8.5; all embryos are being resorbed at E8.5

embryo

increased embryonic tissue cell apoptosis ( J:62177 )

• all E6.5 embryos show high levels of apoptotic cells (10-100% TUNEL-positive cells) in distal regions (U-shaped area encompassing 25% of the egg cylinder and involves embryonic ectoderm and visceral endoderm); apoptosis in proximal region is similar to that seen in controls

abnormal embryo development ( J:62177 )

• at E6.5 homozygous embryos appear identical to controls, but at E7.5, about 25% of embryos are morphologically abnormal

failure to gastrulate ( J:62177 )

• embryos fail to gastrulate

embryonic growth arrest ( J:62177 )

• at E7.5, abnormal embryos fail to differentiate beyond egg cylinder stage; embryos have egg cylinders composed of discrete ectodermal and endodermal layers with no evidence of mesoderm on mixed or 129Sv inbred backgrounds

embryonic growth retardation ( J:62177 )

absent mesoderm ( J:62177 )

• no evidence of mesoderm at E7.5

abnormal embryonic-extraembryonic boundary morphology ( J:62177 )

• at E7.5, abnormal embryos show indefinite boundaries between embryonic and extraembryonic regions

growth/size/body

embryonic growth retardation ( J:62177 )

cellular

increased embryonic tissue cell apoptosis ( J:62177 )

• all E6.5 embryos show high levels of apoptotic cells (10-100% TUNEL-positive cells) in distal regions (U-shaped area encompassing 25% of the egg cylinder and involves embryonic ectoderm and visceral endoderm); apoptosis in proximal region is similar to that seen in controls

decreased cell proliferation ( J:62177 )

• proliferation decrease is inversely correlated with elevated apoptosis severity in distal regions

Genotype
MGI:3814418

ht2

• Allelic Composition: Gclc^tm1Mwl/Gclc⁺
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

normal phenotype

no abnormal phenotype detected ( J:62177 )

• mice are show no obvious abnormalities and are fertile