Analysis Tools
mortality/aging
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• viability of homozygotes at weaning is only 50%
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• Background Sensitivity: after 5-7 generations of backcross, frequency of homozygotes from heterozygous intercrosses drops to 14% from expected 25%
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growth/size/body
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• mutants display reduced BMI relative to wild-type
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• at N5 or N6, males maintained on a 10% fat chow diet weigh 23% less than wild-type at weaning, and females weigh 16% less than wild-type females
• weight differences persist in adult animals which exhibit a 27% (males) or 14% (females) decrease at 9-11 months
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reproductive system
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• fertility is reduced proportionately with increasing Gpdtm1Lbr dosage
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• at N5 or N6 generations, litter size is reduced; homozygous crosses produce 4.6 pups and heterozygous crosses have 5.9 pups/litter vs 7.3 from wild-type crosses
• male homozygotes crossed with wild-type produce 7.4 pups/litter while male homozygotes crossed with female heterozygotes have 5.4 pups/litter
• female homozygotes crossed with wild-type have 6.5 pups/litter while female homozygotes crossed to male heterozygotes produce only 3.6 pups/litter
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adipose tissue
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• 40% reduction in weight
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• white adipose tissue weight is reduced ~40% in males
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• in females, parametrial fat pad white adipose tissue weight is reduced by ~40%
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homeostasis/metabolism
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• liver and muscle metabolites are normal in adult animals; adult knockouts show normal cold tolerance, normal circadian rhythm in body temperature and normal temperature increase to thyroid hormone administration
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