Phenotypes associated with this allele

• Allele Symbol: Hrh3^tm1Twl
• Allele Name: targeted mutation 1, Timothy W Lovenberg
• Allele ID: MGI:2387415
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hrh3^tm1Twl/Hrh3^tm1Twl	B6.129X1-Hrh3^tm1Twl	MGI:3775276
hm2	Hrh3^tm1Twl/Hrh3^tm1Twl	involves: 129P2/OlaHsd	MGI:3775272
hm3	Hrh3^tm1Twl/Hrh3^tm1Twl	involves: 129P2/OlaHsd * C57BL/6J	MGI:3607904

Genotype
MGI:3775276

hm1

• Allelic Composition: Hrh3^tm1Twl/Hrh3^tm1Twl
• Genetic Background: B6.129X1-Hrh3^tm1Twl

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased susceptibility to experimental autoimmune encephalomyelitis ( J:122269 )

• the acute-early phase cumulative disease scores and number of days affected by experimental autoimmune encephalomyelitis (EAE) is increased compared to in wild-type mice

• at day 12 following induction mice exhibit greater EAE pathology than wild-type mice

• however, pathogenesis of EAE at day 30 is similar to that observed in wild-type mice

• during induction of EAE, mice exhibit increased blood-brain barrier permeability compared to wild-type mice

nervous system

impaired blood-brain barrier function ( J:122269 )

• during induction of EAE, mice exhibit increased blood-brain barrier permeability compared to wild-type mice

cardiovascular system

impaired blood-brain barrier function ( J:122269 )

• during induction of EAE, mice exhibit increased blood-brain barrier permeability compared to wild-type mice

Genotype
MGI:3775272

hm2

• Allelic Composition: Hrh3^tm1Twl/Hrh3^tm1Twl
• Genetic Background: involves: 129P2/OlaHsd

nervous system

abnormal synaptic norepinephrine release ( J:103897 )

• norepinepherine release from cardiac synaptosomes is increased 25% to 35% above basal levels, which is 60% higher than in wild-type mice

• in response to ischemic conditions, norephinepehrine overflow is 2-fold greater than in wild-type mice

• however, Adora1-mediated modulation of norepinepherine release is retained

cardiovascular system

abnormal cardiovascular system physiology ( J:103897 )

• in response to ischemic conditions, norephinepehrine overflow from cardiac synaptosomes is 2-fold greater than in wild-type mice

immune system

immune system phenotype ( J:132140 )

N • unlike mice homozygous for a null allele of Hrh4, mice display normal mast cells stimulation and calcium mobilization

Genotype
MGI:3607904

hm3

• Allelic Composition: Hrh3^tm1Twl/Hrh3^tm1Twl
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

nervous system

abnormal nervous system physiology ( J:79168 )

• decreased levels of cortical histamine

behavior/neurological

abnormal spatial learning ( J:89633 )

• improved spatial learning and memory in Barnes maze test

abnormal anxiety-related response ( J:79168 )

• resistant to amnesic effect of scopolamine in passive avoidance tests, remember aversive stimuli

• analgesic effects of scopolamine unaffected

decreased anxiety-related response ( J:89633 )

• less anxiety in elevated plus and zero mazes although anxiety levels were normal in open field tests

increased anxiety-related response ( J:89633 )

• higher startle responses

abnormal locomotor activation ( J:79168 )

• reduced spontaneous activity in open field tests and wheel running

• reduced activity primarily in dark phase of circadian cycle

• wheel running in light phase is normal

• 22% overall reduction in activity

• lower sensitivity to activity reduction by methamphetamine

homeostasis/metabolism

abnormal circadian temperature homeostasis ( J:79168 )

• impaired body temperature elevation during the dark phase of circadian cycle

abnormal hormone level ( J:89633 )

• higher levels of arginine vasopressin in the central nucleus and basolateral nucleus of the amygdala